دورية أكاديمية
Detection of Trypanosoma cruzi DTUs TcI and TcIV in two outbreaks of orally-transmitted Chagas disease in the Northern region of Brazil.
العنوان: | Detection of Trypanosoma cruzi DTUs TcI and TcIV in two outbreaks of orally-transmitted Chagas disease in the Northern region of Brazil. |
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المؤلفون: | Freitas VLT; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil., Piotto MR; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil., Esper HR; Universidade de São Paulo, Faculdade de Medicina, Núcleo de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Secretaria Municipal de Saúde de Santarém, Hospital Municipal de Santarém, Santarém, Pará, Brazil., Nakanishi EYS; Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Investigação Médica em Imunologia (LIM48), São Paulo, São Paulo, Brazil., Fonseca CA; Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Investigação Médica em Imunologia (LIM48), São Paulo, São Paulo, Brazil., Assy JGPL; Universidade de São Paulo, Faculdade de Medicina, Núcleo de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Secretaria Municipal de Saúde de Santarém, Hospital Municipal de Santarém, Santarém, Pará, Brazil., Berreta OCP; Universidade de São Paulo, Faculdade de Medicina, Núcleo de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Secretaria Municipal de Saúde de Santarém, Hospital Municipal de Santarém, Santarém, Pará, Brazil., França FOS; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Núcleo de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Investigação Médica em Imunologia (LIM48), São Paulo, São Paulo, Brazil., Lopes MH; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Investigação Médica em Imunologia (LIM48), São Paulo, São Paulo, Brazil. |
المصدر: | Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2023 Jan 16; Vol. 65, pp. e7. Date of Electronic Publication: 2023 Jan 16 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo Country of Publication: Brazil NLM ID: 7507484 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-9946 (Electronic) Linking ISSN: 00364665 NLM ISO Abbreviation: Rev Inst Med Trop Sao Paulo Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Sao Paulo : Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo |
مواضيع طبية MeSH: | Trypanosoma cruzi*/genetics , Chagas Disease*/diagnosis , Chagas Disease*/epidemiology, Infant, Newborn ; Humans ; Female ; Brazil/epidemiology ; Disease Outbreaks ; RNA, Ribosomal ; Genotype |
مستخلص: | This study describes the laboratory investigation of two acute Chagas disease outbreaks that occurred in the riverside communities of Marimarituba and Cachoeira do Arua, in the Santarem municipality, Para State, located in the Northern region of Brazil, and occurred in March 2016 and August 2017, respectively. The generation of data regarding the diversity of Trypanosoma cruzi parasites circulating in the Amazon region is key for understanding the emergence and expansion of Chagas disease. This study aimed to identify T. cruzi Discrete Typing Units (DTUs) involved in two outbreaks of acute Chagas disease (ACD) directly from the patient's biological sample. Nested and multiplex PCR targeting the 24Sα (rRNA) and mini-exon genes, respectively, were used to identify T. cruzi DTU in blood samples from patients diagnosed with ACD. The samples with positive cPCR were submitted for analysis for T. cruzi DTUs, which included 13 samples from the patients with ACD by oral transmission and two samples collected from two newborns of two women with ACD, from Marimarituba and Cachoeira do Arua. The samples were classified as T. cruzi TcIV, from Marimarituba's outbreak, and T. cruzi TcI, from Cachoeira do Arua's outbreak. The molecular identification of T. cruzi may increase understanding of the role of this parasite in Chagas disease's emergence within the Amazon region, contributing to the improvement of the management of this important, but also neglected, disease. |
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المشرفين على المادة: | 0 (RNA, Ribosomal) |
تواريخ الأحداث: | Date Created: 20230118 Date Completed: 20230119 Latest Revision: 20230202 |
رمز التحديث: | 20230202 |
مُعرف محوري في PubMed: | PMC9870254 |
DOI: | 10.1590/S1678-9946202365007 |
PMID: | 36651468 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1678-9946 |
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DOI: | 10.1590/S1678-9946202365007 |