دورية أكاديمية
أعرض في EDS

Detection of Trypanosoma cruzi DTUs TcI and TcIV in two outbreaks of orally-transmitted Chagas disease in the Northern region of Brazil.

التفاصيل البيبلوغرافية
العنوان: Detection of Trypanosoma cruzi DTUs TcI and TcIV in two outbreaks of orally-transmitted Chagas disease in the Northern region of Brazil.
المؤلفون: Freitas VLT; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil., Piotto MR; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil., Esper HR; Universidade de São Paulo, Faculdade de Medicina, Núcleo de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Secretaria Municipal de Saúde de Santarém, Hospital Municipal de Santarém, Santarém, Pará, Brazil., Nakanishi EYS; Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Investigação Médica em Imunologia (LIM48), São Paulo, São Paulo, Brazil., Fonseca CA; Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Investigação Médica em Imunologia (LIM48), São Paulo, São Paulo, Brazil., Assy JGPL; Universidade de São Paulo, Faculdade de Medicina, Núcleo de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Secretaria Municipal de Saúde de Santarém, Hospital Municipal de Santarém, Santarém, Pará, Brazil., Berreta OCP; Universidade de São Paulo, Faculdade de Medicina, Núcleo de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Secretaria Municipal de Saúde de Santarém, Hospital Municipal de Santarém, Santarém, Pará, Brazil., França FOS; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Núcleo de Medicina Tropical, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Investigação Médica em Imunologia (LIM48), São Paulo, São Paulo, Brazil., Lopes MH; Universidade de São Paulo, Faculdade de Medicina, Departamento de Moléstias Infecciosas e Parasitárias, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, Laboratório de Investigação Médica em Imunologia (LIM48), São Paulo, São Paulo, Brazil.
المصدر: Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2023 Jan 16; Vol. 65, pp. e7. Date of Electronic Publication: 2023 Jan 16 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo Country of Publication: Brazil NLM ID: 7507484 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-9946 (Electronic) Linking ISSN: 00364665 NLM ISO Abbreviation: Rev Inst Med Trop Sao Paulo Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Sao Paulo : Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo
مواضيع طبية MeSH: Trypanosoma cruzi*/genetics , Chagas Disease*/diagnosis , Chagas Disease*/epidemiology, Infant, Newborn ; Humans ; Female ; Brazil/epidemiology ; Disease Outbreaks ; RNA, Ribosomal ; Genotype
مستخلص: This study describes the laboratory investigation of two acute Chagas disease outbreaks that occurred in the riverside communities of Marimarituba and Cachoeira do Arua, in the Santarem municipality, Para State, located in the Northern region of Brazil, and occurred in March 2016 and August 2017, respectively. The generation of data regarding the diversity of Trypanosoma cruzi parasites circulating in the Amazon region is key for understanding the emergence and expansion of Chagas disease. This study aimed to identify T. cruzi Discrete Typing Units (DTUs) involved in two outbreaks of acute Chagas disease (ACD) directly from the patient's biological sample. Nested and multiplex PCR targeting the 24Sα (rRNA) and mini-exon genes, respectively, were used to identify T. cruzi DTU in blood samples from patients diagnosed with ACD. The samples with positive cPCR were submitted for analysis for T. cruzi DTUs, which included 13 samples from the patients with ACD by oral transmission and two samples collected from two newborns of two women with ACD, from Marimarituba and Cachoeira do Arua. The samples were classified as T. cruzi TcIV, from Marimarituba's outbreak, and T. cruzi TcI, from Cachoeira do Arua's outbreak. The molecular identification of T. cruzi may increase understanding of the role of this parasite in Chagas disease's emergence within the Amazon region, contributing to the improvement of the management of this important, but also neglected, disease.
References: Acta Trop. 2018 Aug;184:38-52. (PMID: 28941731)
Trop Med Int Health. 2010 Sep;15(9):1049-51. (PMID: 20579319)
Mol Biochem Parasitol. 1991 Oct;48(2):211-21. (PMID: 1662334)
Rev Inst Med Trop Sao Paulo. 2021 Jun 18;63:e48. (PMID: 34161554)
PLoS One. 2020 Dec 2;15(12):e0243008. (PMID: 33264350)
Trans R Soc Trop Med Hyg. 2002 Jul-Aug;96(4):378-82. (PMID: 12497973)
Expert Rev Anti Infect Ther. 2015 Aug;13(8):995-1029. (PMID: 26162928)
Mol Biochem Parasitol. 1993 Nov;62(1):45-52. (PMID: 8114825)
Acta Trop. 2015 Nov;151:166-77. (PMID: 26200788)
Mem Inst Oswaldo Cruz. 2015 Nov;110(7):825-30. (PMID: 26560976)
Parasit Vectors. 2014 Jun 11;7:267. (PMID: 24916362)
Int J Parasitol. 2009 Apr;39(5):615-23. (PMID: 19041313)
PLoS One. 2012;7(7):e41284. (PMID: 22848457)
PLoS Negl Trop Dis. 2016 Aug 29;10(8):e0004792. (PMID: 27571035)
Int J Infect Dis. 2015 Oct;39:68-75. (PMID: 26327123)
Clin Infect Dis. 2012 Mar;54(6):845-52. (PMID: 22238161)
Mem Inst Oswaldo Cruz. 2009 Nov;104(7):1051-4. (PMID: 20027478)
Rev Inst Med Trop Sao Paulo. 2019 May 6;61:e27. (PMID: 31066753)
Trans R Soc Trop Med Hyg. 2001 Jan-Feb;95(1):97-9. (PMID: 11280078)
المشرفين على المادة: 0 (RNA, Ribosomal)
تواريخ الأحداث: Date Created: 20230118 Date Completed: 20230119 Latest Revision: 20230202
رمز التحديث: 20230202
مُعرف محوري في PubMed: PMC9870254
DOI: 10.1590/S1678-9946202365007
PMID: 36651468
قاعدة البيانات: MEDLINE
الوصف
تدمد:1678-9946
DOI:10.1590/S1678-9946202365007