دورية أكاديمية
A novel model of subretinal edema induced by DL-alpha aminoadipic acid.
| العنوان: | A novel model of subretinal edema induced by DL-alpha aminoadipic acid. |
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| المؤلفون: | Xie Z; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China., Wu X; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China., Cheng R; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China., Huang J; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China., Wang X; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China., Shi Q; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China., Xu B; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China., Paulus YM; Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan, Ann Arbor, MI, USA. Electronic address: ypaulus@med.umich.edu., Yuan S; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China. Electronic address: songtaoyuan@njmu.edu.cn., Liu Q; Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China. Electronic address: liuqh@njmu.edu.cn. |
| المصدر: | Experimental eye research [Exp Eye Res] 2023 Mar; Vol. 228, pp. 109388. Date of Electronic Publication: 2023 Jan 15. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 0370707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0007 (Electronic) Linking ISSN: 00144835 NLM ISO Abbreviation: Exp Eye Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Academic Press Original Publication: London. |
| مواضيع طبية MeSH: | 2-Aminoadipic Acid* , Edema*, Mice ; Animals ; Rabbits ; Mice, Inbred C57BL ; Fluorescein Angiography/methods ; Blood-Retinal Barrier/physiology ; Tomography, Optical Coherence/methods |
| مستخلص: | In this study we described a new model of subretinal edema induced by single intraocular injection of DL-alpha-aminoadipic acid (DLAAA) that can be employed to study the mechanism of retinal edema and test the efficacy or potential toxicity of treatments. The progression of subretinal edema was evaluated by fundus photography, fluorescein angiography and optical coherence tomography for up to 4 weeks following DLAAA injection. The VEGF, IL-6, TNF-α, Occludin, ZO-1, AQP4, Kir4.1, GFAP and GS levels were examined in DLAAA models by immunostaining, immumohistochemical staining and Western blot. Additionally, bulk RNA-seq was used to detect the mechanism involved in DLAAA-induced retinal Müller cellular injuries. In vivo and vitro assays were further conducted to confirm the sequencing results. Subretinal edema was successfully induced by DLAAA in New Zealand White rabbits (1.29 mg/eye) and C57BL/6 mice (50 or 100 μg/eye). Our results demonstrated that the disruption of blood-retinal-barrier, including vascular hyperpermeability, inflammation, and Müller cell dysfunction of fluid clearance, was involved in subretinal edema formation in the model. Bulk RNA-seq and in vitro studies indicated the activation of p38 MAPK signaling pathway in DLAAA models. This DLAAA-induced subretinal edema model can be used for mechanistic studies or drug screening. Competing Interests: Declaration of competing interest None of the authors has a conflict of interest relevant to this manuscript. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: DL-Alpha-aminoadipic acid; DLAAA; Mouse model; Rabbit model; Retinal edema; p38 MAPK |
| المشرفين على المادة: | 1K7B1OED4N (2-Aminoadipic Acid) |
| تواريخ الأحداث: | Date Created: 20230118 Date Completed: 20230228 Latest Revision: 20230303 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.exer.2023.109388 |
| PMID: | 36652968 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0007 |
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| DOI: | 10.1016/j.exer.2023.109388 |