دورية أكاديمية
Improvement of bovine pestiviral diagnosis by the development of a cost-effective method for detecting viral RNA in fresh specimens and samples spotted in filter papers.
| العنوان: | Improvement of bovine pestiviral diagnosis by the development of a cost-effective method for detecting viral RNA in fresh specimens and samples spotted in filter papers. |
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| المؤلفون: | Favaro PM; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe, Argentina. Electronic address: pfavaro@fcv.unl.edu.ar., Molineri A; Instituto de Investigación de la Cadena Láctea (INTA-CONICET), Estación Experimental Agropecuaria Rafaela, Santa Fe, Argentina., Dus Santos MJ; Instituto de Virología e Innovaciones Tecnológicas (IVIT) CICVyA, INTA Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Buenos Aires, Argentina., Calvinho LF; Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe, Argentina; Instituto de Investigación de la Cadena Láctea (INTA-CONICET), Estación Experimental Agropecuaria Rafaela, Santa Fe, Argentina., Pecora A; Instituto de Virología e Innovaciones Tecnológicas (IVIT) CICVyA, INTA Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Buenos Aires, Argentina. |
| المصدر: | Revista Argentina de microbiologia [Rev Argent Microbiol] 2023 Apr-Jun; Vol. 55 (2), pp. 167-175. Date of Electronic Publication: 2023 Jan 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Asociación Argentina de Microbiología Country of Publication: Argentina NLM ID: 8002834 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0325-7541 (Print) Linking ISSN: 03257541 NLM ISO Abbreviation: Rev Argent Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Buenos Aires, Asociación Argentina de Microbiología. |
| مواضيع طبية MeSH: | Pestivirus Infections*/diagnosis , Pestivirus Infections*/veterinary , Diarrhea Viruses, Bovine Viral*/genetics, Animals ; Cattle ; RNA, Viral/genetics ; Cost-Benefit Analysis |
| مستخلص: | Bovine pestiviruses are the causative agents of bovine viral diarrhea, a disease that causes severe economic losses in cattle. The aim of this study was to improve their diagnosis by developing a RT-qPCR to detect bovine pestiviruses A, B and H; and to set up a protocol for collecting, shipping and preserving bovine pestiviral RNA on filter papers. The developed RT-qPCR showed high sensitivity in detecting these viruses in different matrices: viral stocks, semen and serum samples. With regard to the possibility of using the technique to test serum pools, it was possible to identify a positive serum sample within a pool containing 30 sera. In addition to evaluating the qPCR from fresh samples, the use of filter papers to sow bovine samples was analyzed. The sampling method on two different filter papers using bovine blood drops was a useful alternative for diagnostic purposes and allowed to preserve pestiviral RNA for up to 12 months under refrigeration. (Copyright © 2022 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bovine pestiviruses; Diagnosis; Diagnóstico; Filter papers; Papeles de filtro; Pestivirus bovinos; RT-qPCR |
| المشرفين على المادة: | 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20230119 Date Completed: 20230619 Latest Revision: 20230619 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.ram.2022.10.002 |
| PMID: | 36658065 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0325-7541 |
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| DOI: | 10.1016/j.ram.2022.10.002 |