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Domain Truncation in Hispidin Synthase Orthologs from Non-Bioluminescent Fungi Does Not Lead to Hispidin Biosynthesis.

التفاصيل البيبلوغرافية
العنوان: Domain Truncation in Hispidin Synthase Orthologs from Non-Bioluminescent Fungi Does Not Lead to Hispidin Biosynthesis.
المؤلفون: Palkina KA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Planta LLC., 121205 Moscow, Russia., Balakireva AV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Planta LLC., 121205 Moscow, Russia., Belozerova OA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Institute of Translational Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia., Chepurnykh TV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia., Markina NM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Planta LLC., 121205 Moscow, Russia., Kovalchuk SI; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia., Tsarkova AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Institute of Translational Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia., Mishin AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Planta LLC., 121205 Moscow, Russia., Yampolsky IV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Institute of Translational Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia., Sarkisyan KS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Planta LLC., 121205 Moscow, Russia.; Synthetic Biology Group, MRC London Institute of Medical Sciences, London W12 0NN, UK.; Institute of Clinical Sciences, Faculty of Medicine and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Jan 10; Vol. 24 (2). Date of Electronic Publication: 2023 Jan 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Polyketide Synthases*/genetics , Polyketide Synthases*/metabolism , Pyrones*, Nitric Oxide Synthase/metabolism ; Fungi/genetics ; Fungi/metabolism ; Hydro-Lyases/metabolism
مستخلص: Hispidin is a polyketide found in plants and fungi. In bioluminescent fungi, hispidin serves as a precursor of luciferin and is produced by hispidin synthases. Previous studies revealed that hispidin synthases differ in orthologous polyketide synthases from non-bioluminescent fungi by the absence of two domains with predicted ketoreductase and dehydratase activities. Here, we investigated the hypothesis that the loss of these domains in evolution led to the production of hispidin and the emergence of bioluminescence. We cloned three orthologous polyketide synthases from non-bioluminescent fungi, as well as their truncated variants, and assessed their ability to produce hispidin in a bioluminescence assay in yeast. Interestingly, expression of the full-length enzyme hsPKS resulted in dim luminescence, indicating that small amounts of hispidin are likely being produced as side products of the main reaction. Deletion of the ketoreductase and dehydratase domains resulted in no luminescence. Thus, domain truncation by itself does not appear to be a sufficient step for the emergence of efficient hispidin synthases from orthologous polyketide synthases. At the same time, the production of small amounts of hispidin or related compounds by full-length enzymes suggests that ancestral fungal species were well-positioned for the evolution of bioluminescence.
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معلومات مُعتمدة: MC_UP_1605/9 United Kingdom MRC_ Medical Research Council
فهرسة مساهمة: Keywords: bioluminescence; caffeic acid; fungi; hispidin; polyketide synthases; α-pyrones
المشرفين على المادة: SSJ18CG55E (hispidin)
79956-01-7 (Polyketide Synthases)
0 (Pyrones)
EC 1.14.13.39 (Nitric Oxide Synthase)
EC 4.2.1.- (Hydro-Lyases)
تواريخ الأحداث: Date Created: 20230121 Date Completed: 20230124 Latest Revision: 20240313
رمز التحديث: 20240313
مُعرف محوري في PubMed: PMC9866795
DOI: 10.3390/ijms24021317
PMID: 36674833
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms24021317