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The Indirect ELISA and Monoclonal Antibody against African Swine Fever Virus p17 Revealed Efficient Detection and Application Prospects.

التفاصيل البيبلوغرافية
العنوان: The Indirect ELISA and Monoclonal Antibody against African Swine Fever Virus p17 Revealed Efficient Detection and Application Prospects.
المؤلفون: Li L; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China., Qiao S; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.; College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China., Li G; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China., Tong W; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China., Dong S; College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China., Liu J; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China., Guo Z; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China., Zheng H; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China., Zhao R; Xiamen Center for Animal Disease Control and Prevention, Xiamen 361009, China., Tong G; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China., Zhou Y; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China., Gao F; Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China.
المصدر: Viruses [Viruses] 2022 Dec 23; Vol. 15 (1). Date of Electronic Publication: 2022 Dec 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: African Swine Fever Virus* , African Swine Fever*/diagnosis , African Swine Fever*/prevention & control , African Swine Fever*/epidemiology, Swine ; Animals ; Cricetinae ; Viral Proteins ; Antibodies, Monoclonal ; Cricetulus ; Vaccines, Synthetic ; Enzyme-Linked Immunosorbent Assay ; Antibodies, Viral
مستخلص: Since 2018, the outbreak and prevalence of the African swine fever virus (ASFV) in China have caused huge economic losses. Less virulent ASFVs emerged in 2020, which led to difficulties and challenges for early diagnosis and control of African swine fever (ASF) in China. An effective method of monitoring ASFV antibodies and specific antibodies against ASFV to promote the development of prevention techniques are urgently needed. In the present study, ASFV p17 was successfully expressed in CHO cells using a suspension culture system. An indirect enzyme-linked immunosorbent assay (ELISA) based on purified p17 was established and optimized. The monoclonal antibody (mAb) against p17 recognized a conservative linear epitope ( 3 TETSPLLSH 11 ) and exhibited specific reactivity, which was conducive to the identification of recombinant porcine reproductive and respiratory syndrome virus (PRRSV) expressing p17. The ELISA method efficiently detected clinical ASFV infection and effectively monitored the antibody levels in vivo after recombinant PRRSV live vector virus expressing p17 vaccination. Overall, the determination of the conserved linear epitope of p17 would contribute to the in-depth exploration of the biological function of the ASFV antigen protein. The indirect ELISA method and mAb against ASFV p17 revealed efficient detection and promising application prospects, making them ideal for epidemiological surveillance and vaccine research on ASF.
Competing Interests: The authors declare that they have no conflict of interest associated with this study.
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فهرسة مساهمة: Keywords: ASFV p17; CHO cells; epitope; indirect ELISA; recombinant PRRSV
المشرفين على المادة: 0 (Viral Proteins)
0 (Antibodies, Monoclonal)
0 (Vaccines, Synthetic)
0 (Antibodies, Viral)
تواريخ الأحداث: Date Created: 20230121 Date Completed: 20230124 Latest Revision: 20231116
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9865993
DOI: 10.3390/v15010050
PMID: 36680090
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v15010050