دورية أكاديمية
High-efficiency novel extraction process of target polyphenols using enzymes in hydroalcoholic media.
| العنوان: | High-efficiency novel extraction process of target polyphenols using enzymes in hydroalcoholic media. |
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| المؤلفون: | Piazza DM; Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Facultad de Ciencias Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina., Romanini D; Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Facultad de Ciencias Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina.; Facultad de Ciencias Bioquímicas Y Farmacéuticas, Departamento de Tecnología, UNR, Rosario, Argentina., Meini MR; Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Facultad de Ciencias Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), Rosario, Argentina. meini@iprobyq-conicet.gob.ar.; Área Biofísica, Facultad de Ciencias Bioquímicas Y Farmacéuticas, UNR, Rosario, Argentina. meini@iprobyq-conicet.gob.ar.; IPROBYQ-CONICET, Facultad de Ciencias Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario, Mitre 1998 - S2000FWF, Rosario, Santa Fe, Argentina. meini@iprobyq-conicet.gob.ar. |
| المصدر: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Feb; Vol. 107 (4), pp. 1205-1216. Date of Electronic Publication: 2023 Jan 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer International, c1984- |
| مواضيع طبية MeSH: | Polyphenols* , Cellulases*, Polygalacturonase ; Solvents ; Ethanol ; Plant Extracts ; Antioxidants |
| مستخلص: | Agro-industrial by-products are a sustainable source of natural additives that can replace the synthetic ones in the food industry. Grape pomace is an abundant by-product that contains about 70% of the grape's polyphenols. Polyphenols are natural antioxidants with multiple health-promoting properties. They are secondary plant metabolites with a wide range of solubilities. Here, a novel extraction process of these compounds was developed using enzymes that specifically liberates target polyphenols in the appropriate hydroalcoholic mixture. Tannase, cellulase, and pectinase retained 22, 60, and 52% of their activity, respectively, in ethanol 30% v/v. Therefore, extractions were tested in ethanol concentrations between 0 and 30% v/v. Some of these enzymes presented synergistic effects in the extraction of specific polyphenols. Maximum yield of gallic acid was obtained using tannase and pectinase enzymes in ethanol 10% v/v (49.56 ± 0.01 mg L -1 h -1 ); in the case of p-coumaric acid, by cellulase and pectinase treatment in ethanol 30% v/v (7.72 ± 0.26 mg L -1 h -1 ), and in the case of trans-resveratrol, by pectinase treatment in ethanol 30% v/v (0.98 ± 0.04 mg L -1 h -1 ). Also, the effect of enzymes and solvent polarity was analysed for the extraction of malvidin-3-O-glucoside, syringic acid, and quercetin. Previous studies were mainly focused on the maximization of total polyphenols extraction yields, being the polyphenolic profile the consequence but not the driving force of the optimization. In the present study, the basis of a platform for a precise extraction of the desire polyphenols is provided. KEY POINTS: • Enzymes can be used up to ethanol 30% v/v. • The specific enzymes' action determines the polyphenolic profile of the extracts. • The yields obtained of target polyphenols are competitive. (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| معلومات مُعتمدة: | PICT 2020 SERIE A-00445 Fondo para la Investigación Científica y Tecnológica; PIP 11220200103120 Consejo Nacional de Investigaciones Científicas y Técnicas; DTT-2021-065 Agencia Santafesina de Ciencia, Tecnología e Innovación; 0020190100061 Universidad Nacional de Rosario |
| فهرسة مساهمة: | Keywords: Enzymes; Gallic acid; Grape pomace; Polyphenols; Trans-Resveratrol; p-Coumaric acid |
| المشرفين على المادة: | 0 (Polyphenols) EC 3.2.1.15 (Polygalacturonase) 0 (Solvents) 3K9958V90M (Ethanol) 0 (Plant Extracts) 0 (Antioxidants) EC 3.2.1.- (Cellulases) |
| تواريخ الأحداث: | Date Created: 20230121 Date Completed: 20230207 Latest Revision: 20230207 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/s00253-023-12386-7 |
| PMID: | 36680585 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1432-0614 |
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| DOI: | 10.1007/s00253-023-12386-7 |