دورية أكاديمية
أعرض في EDS

Comet assay in Aegla platensis (Decapoda: Anomura) using a non-lethal hemolymph field sampling for in situ monitoring of freshwater genotoxicity.

التفاصيل البيبلوغرافية
العنوان: Comet assay in Aegla platensis (Decapoda: Anomura) using a non-lethal hemolymph field sampling for in situ monitoring of freshwater genotoxicity.
المؤلفون: Goldoni A; Feevale University, ERS-239, 2755, Novo Hamburgo, Rio Grande do Sul, Brazil., Pacheco MR; Feevale University, ERS-239, 2755, Novo Hamburgo, Rio Grande do Sul, Brazil., da Silva LB; Feevale University, ERS-239, 2755, Novo Hamburgo, Rio Grande do Sul, Brazil. lucianosilva@feevale.br.
المصدر: Ecotoxicology (London, England) [Ecotoxicology] 2023 Mar; Vol. 32 (2), pp. 160-165. Date of Electronic Publication: 2023 Jan 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Kluwer Academic Publishers Country of Publication: United States NLM ID: 9885956 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-3017 (Electronic) Linking ISSN: 09639292 NLM ISO Abbreviation: Ecotoxicology Subsets: MEDLINE
أسماء مطبوعة: Publication: 1999- : Boston : Kluwer Academic Publishers
Original Publication: London : Chapman & Hall,
مواضيع طبية MeSH: Anomura*/genetics, Animals ; Comet Assay ; Hemolymph ; Fresh Water ; DNA Damage
مستخلص: This study aimed to apply the comet assay on Aegla platensis crabs as a suitable non-destructive approach for in situ monitoring of freshwater genotoxicity. Animals were captured during four sampling periods in a stream under minor anthropogenic impacts in Southern Brazil. Crabs were captured with a hand net, then the hemolymph samples were collected, and the animals were released into the stream after a 20-min recovery time. Hemolymph samples were transported to the laboratory and used to perform the alkaline comet assay. Results showed an intermediate level in the DNA damage index (range 107.3-165.0 arbitrary unit). No significant differences were observed among the different sampling periods. Hemolymph was successfully used as a non-lethal source of biological samples, and the comet assay using A. platensis proved to be a feasible approach for genotoxicity studies.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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فهرسة مساهمة: Keywords: Biomarkers; Comet assay; Crustaceans; Genotoxicity; In situ monitoring
تواريخ الأحداث: Date Created: 20230121 Date Completed: 20230314 Latest Revision: 20230314
رمز التحديث: 20240628
DOI: 10.1007/s10646-023-02627-w
PMID: 36680665
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-3017
DOI:10.1007/s10646-023-02627-w