دورية أكاديمية
Characterisation of native and decellularised porcine tendon under tension and compression: A closer look at glycosaminoglycan contribution to tendon mechanics.
| العنوان: | Characterisation of native and decellularised porcine tendon under tension and compression: A closer look at glycosaminoglycan contribution to tendon mechanics. |
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| المؤلفون: | Solis-Cordova J; Institute of Medical and Biological Engineering, School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom; Institute of Medical and Biological Engineering, School of Mechanical Engineering, Faculty of Engineering and Physical Sciences, University of Leeds, Leeds, United Kingdom. Electronic address: bsbjsc@leeds.ac.uk., Edwards JH; Institute of Medical and Biological Engineering, School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom., Fermor HL; Institute of Medical and Biological Engineering, School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom., Riches P; Department of Biomedical Engineering, Faculty of Engineering, University of Strathclyde, Wolfson Centre, Glasgow, United Kingdom., Brockett CL; Institute of Medical and Biological Engineering, School of Mechanical Engineering, Faculty of Engineering and Physical Sciences, University of Leeds, Leeds, United Kingdom., Herbert A; Institute of Medical and Biological Engineering, School of Mechanical Engineering, Faculty of Engineering and Physical Sciences, University of Leeds, Leeds, United Kingdom. |
| المصدر: | Journal of the mechanical behavior of biomedical materials [J Mech Behav Biomed Mater] 2023 Mar; Vol. 139, pp. 105671. Date of Electronic Publication: 2023 Jan 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101322406 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0180 (Electronic) Linking ISSN: 18780180 NLM ISO Abbreviation: J Mech Behav Biomed Mater Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Glycosaminoglycans* , Tendons*, Animals ; Swine ; Anterior Cruciate Ligament ; Collagen ; Physical Phenomena ; Biomechanical Phenomena |
| مستخلص: | Decellularised porcine superflexor tendon (pSFT) has been characterised as a suitable scaffold for anterior cruciate ligament replacement, with dimensions similar to hamstring tendon autograft. However, decellularisation of tissues may reduce or damage extracellular matrix components, leading to undesirable biomechanical changes at a whole tissue scale. Although the role of collagen in tendons is well established, the mechanical contribution of glycosaminoglycans (GAGs) is less evident and could be altered by the decellularisation process. In this study, the contribution of GAGs to the tensile and compressive mechanical properties of pSFT was determined and whether decellularisation affected these properties by reducing GAG content or functionality. PSFTs were either enzymatically treated using chondroitinase ABC to remove GAGs or decellularised using previously established methods. Native, GAG-depleted and decellularised pSFT groups were then subjected to quantitative assays and biomechanical characterisation. In tension, specimens underwent stress relaxation and strength testing. In compression, specimens underwent confined compression testing. The GAG-depleted group was found to have circa 86% reduction of GAG content compared to native and decellularised groups. There was no significant difference in GAG content between native (3.75 ± 0.58 μg/mg) and decellularised (3.40 ± 0.37 μg/mg) groups. Stress relaxation testing discovered the time-independent and time-dependent relaxation moduli of the decellularised group were reduced ≥50% compared to native and GAG-depleted groups. However, viscoelastic behaviour of native and GAG-depleted groups resulted similar. Strength testing discovered no differences between native and GAG-depleted group's properties, albeit a reduction ∼20% for decellularised specimens' linear modulus and tensile strength compared to native tissue. In compression testing, the aggregate modulus was found to be circa 74% lower in the GAG-depleted group than the native and decellularised groups, while the zero-strain permeability was significantly higher in the GAG-depleted group (0.86 ± 0.65 mm 4 /N) than the decellularised group (0.03 ± 0.04 mm 4 /N). The results indicate that GAGs may significantly contribute to the mechanical properties of pSFT in compression, but not in tension. Furthermore, the content and function of GAGs in pSFTs are unaffected by decellularisation and the mechanical properties of the tissue remain comparable to native tissue. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biomechanics; Decellularisation; Glycosaminoglycans; Tendon; Tissue scaffolds |
| المشرفين على المادة: | 0 (Glycosaminoglycans) 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20230122 Date Completed: 20230213 Latest Revision: 20230309 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jmbbm.2023.105671 |
| PMID: | 36682172 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-0180 |
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| DOI: | 10.1016/j.jmbbm.2023.105671 |