دورية أكاديمية
أعرض في EDS

Application of loop mediated isothermal amplification (LAMP) assays for the detection of Onchocerca volvulus , Loa loa and Mansonella perstans in humans and vectors.

التفاصيل البيبلوغرافية
العنوان: Application of loop mediated isothermal amplification (LAMP) assays for the detection of Onchocerca volvulus , Loa loa and Mansonella perstans in humans and vectors.
المؤلفون: Amambo GN; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Innocentia N; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Abong RA; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Fombad FF; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Njouendou AJ; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Department of Biomedical Science, Faculty of Health Sciences, University of Buea, Buea, Cameroon., Nietcho F; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon., Ekanya R; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Kien CA; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Ebai R; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Lenz B; Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany., Ritter M; Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany., Esum ME; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Deribe K; Global Health and Infection Department, Brighton and Sussex Medical School, Brighton, United Kingdom.; School of Public Health, Addis Ababa University, Addis Ababa, Ethiopia., Cho JF; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Beng AA; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Enyong PI; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon., Li Z; New England Biolabs, Ipswich, MA, United States., Hübner MP; Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany.; German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany., Pfarr K; Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany.; German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany., Hoerauf A; Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany.; German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Bonn, Germany.; German-West African Centre for Global Health and Pandemic Prevention (G-WAC), Partner Site Bonn, Bonn, Germany., Carlow C; New England Biolabs, Ipswich, MA, United States., Wanji S; Parasites and Vector Research Unit (PAVRU), Department of Microbiology and Parasitology, University of Buea, Buea, Cameroon.; Research Foundation in Tropical Diseases and Environment (REFOTDE), Buea, Cameroon.
المصدر: Frontiers in tropical diseases [Front Trop Dis] 2023 Jan 09; Vol. 3, pp. 1016176.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101779328 Publication Model: Print Cited Medium: Internet ISSN: 2673-7515 (Electronic) Linking ISSN: 26737515 NLM ISO Abbreviation: Front Trop Dis Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne, Switzerland : Frontiers Media S.A., [2021]-
مستخلص: Conventional diagnosis of filarial infections is based on morphological identification of microfilariae using light microscopy and requires considerable expertise, is time-consuming, and can be subjective. Loop-mediated isothermal amplification (LAMP) has advantages over microscopy or PCR because of its operational simplicity, rapidity and versatility of readout options. LAMP assays represent a major step forward in improved filarial diagnostic tools suitable for low resource settings and field applicability. The study goal was to retrospectively evaluate the performance and suitability of the O-150, RF4, and Mp419 LAMP assays for diagnosing Onchocerca volvulus , Loa loa and Mansonella perstans infections, respectively, in humans and vectors under experimental and natural field conditions. Surveys were conducted in four health districts of Cameroon using skin snip and thick blood film methods to detect skin ( O. volvulus ) and blood ( L. loa and M. perstans ) dwelling microfilaria in humans. Engorged vectors ( Simulium spp ., Chrysops spp ., and Culicoides spp.) were evaluated by LAMP. Dissected, wild-caught vectors were also analyzed. LAMP showed a prevalence of 40.4% ( O. volvulus ), 17.8% ( L. loa ) and 36.6% ( M. perstans ) versus 20.6% ( O. volvulus ), 17.4% ( L. loa ) and 33.8% ( M. perstans ) with microscopy. Simulium spp. were dissected for microscopy and pooled for LAMP. The O-150 LAMP assay infection rate was 4.3% versus 4.1% by microscopy. Chrysops spp. were dissected and analyzed individually in the LAMP assay. The RF4 LAMP assay infection rate was 23.5% versus 3.3% with microscopy. The RF4 LAMP assay also detected parasites in Chrysops spp. fed on low microfilaremic volunteers. The Mp419 LAMP assay infection rate was 0.2% for C. milnei and 0.04% for C. grahamii , while three other species were LAMP-negative. The sensitivity, species specificity, rapidity and ease of its use of these filarial LAMP assays, and validation of their performance in the field support use as alternatives to microscopy as diagnostic and surveillance tools in global health programs aimed to eliminate onchocerciasis.
Competing Interests: Conflict of interest ZL and CC are employees of New England Biolabs, manufacturer of LAMP reagents described in the manuscript. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust; 201900 United Kingdom WT_ Wellcome Trust; 201900/Z/16/Z United Kingdom WT_ Wellcome Trust
فهرسة مساهمة: Keywords: LAMP (loop mediated isothermal amplification); Loa loa; Mansonella perstans; Onchocerca volvulus; diagnostic test
تواريخ الأحداث: Date Created: 20230123 Latest Revision: 20230816
رمز التحديث: 20230816
مُعرف محوري في PubMed: PMC7614089
DOI: 10.3389/fitd.2022.1016176
PMID: 36684508
قاعدة البيانات: MEDLINE
الوصف
تدمد:2673-7515
DOI:10.3389/fitd.2022.1016176