دورية أكاديمية
أعرض في EDS

Role for the metalloproteinase ADAM28 in the control of airway inflammation, remodelling and responsiveness in asthma.

التفاصيل البيبلوغرافية
العنوان: Role for the metalloproteinase ADAM28 in the control of airway inflammation, remodelling and responsiveness in asthma.
المؤلفون: Bendavid G; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium.; Department of Otorhinolaryngology Head and Neck Surgery, University of Liege (ULiege) and Centre Hospitalier Universitaire (CHU) Liege, Liege, Belgium., Hubeau C; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium., Perin F; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium., Gillard A; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium., Nokin MJ; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium., Carnet O; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium., Gerard C; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium., Noel A; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium., Lefebvre P; Department of Otorhinolaryngology Head and Neck Surgery, University of Liege (ULiege) and Centre Hospitalier Universitaire (CHU) Liege, Liege, Belgium., Rocks N; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium., Cataldo D; Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liege (ULiege), Liege, Belgium.; Department of respiratory diseases, University of Liege (ULiege) and Centre Hospitalier Universitaire (CHU) Liege, Liege, Belgium.
المصدر: Frontiers in immunology [Front Immunol] 2023 Jan 05; Vol. 13, pp. 1067779. Date of Electronic Publication: 2023 Jan 05 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Airway Remodeling* , Asthma*/metabolism, Mice ; Animals ; Hyperplasia/pathology ; Bronchoalveolar Lavage Fluid ; Disease Models, Animal ; Lung ; Inflammation/metabolism ; Allergens/metabolism ; Metalloproteases/metabolism
مستخلص: Background: Asthma is characterized by morphological modifications of the airways (inflammation and remodelling) and bronchial hyperresponsiveness. Mechanisms linking these two key features of asthma are still poorly understood. ADAM28 (a disintegrin and metalloproteinase 28) might play a role in asthma pathophysiology. ADAM28 exists as membrane-bound and soluble forms and is mainly expressed by lymphocytes and epithelial cells.
Methods: ADAM28 -/- mice and ADAM28 +/+ counterparts were sensitized and exposed to ovalbumin (OVA). Airway responsiveness was measured using the flexiVent ® system. After sacrifice, bronchoalveolar lavage (BAL) was performed and lungs were collected for analysis of airway inflammation and remodelling.
Results: The expression of the soluble form of ADAM28 was lower in the lungs of OVA-exposed mice (as compared to PBS-exposed mice) and progressively increased in correlation with the duration of allergen exposure. In lungs of ADAM28 -/- mice exposed to allergens, the proportion of Th2 cells among CD 4 + cells and the number of B cells were decreased. Bronchial responsiveness was lower in ADAM28 -/- mice exposed to allergens and similar to the responsiveness of sham-challenged mice. Similarly, features of airway remodelling (collagen deposition, smooth muscle hyperplasia, mucous hyperplasia) were significantly less developed in OVA-exposed ADAM28 -/- animals in sharp contrasts to ADAM28 +/+ . In addition, we report the first evidence of ADAM28 RNA expression by lung fibroblasts and we unveil a decreased capacity of lung fibroblasts extracted from OVA-exposed ADAM28 -/- mice to proliferate as compared to those extracted from OVA-exposed ADAM28 +/+ suggesting a direct contribution of this enzyme to the modulation of airway remodelling.
Conclusion: These results suggest that ADAM28 might be a key contributor to the pathophysiology of asthma.
Competing Interests: The authors declare that the research presented in this article was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Bendavid, Hubeau, Perin, Gillard, Nokin, Carnet, Gerard, Noel, Lefebvre, Rocks and Cataldo.)
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فهرسة مساهمة: Keywords: ADAM28; adamalysins; airway remodelling; asthma; mouse model; proteases
المشرفين على المادة: 0 (Allergens)
EC 3.4.- (Metalloproteases)
تواريخ الأحداث: Date Created: 20230123 Date Completed: 20230124 Latest Revision: 20230131
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9851272
DOI: 10.3389/fimmu.2022.1067779
PMID: 36685493
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-3224
DOI:10.3389/fimmu.2022.1067779