دورية أكاديمية
أعرض في EDS

Leveraging an advanced simulated moving bed approach to achieve 3-component separation for enhanced impurity removal in a non-affinity cation exchange capture step.

التفاصيل البيبلوغرافية
العنوان: Leveraging an advanced simulated moving bed approach to achieve 3-component separation for enhanced impurity removal in a non-affinity cation exchange capture step.
المؤلفون: Chen SW; Downstream Processing Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore, Singapore., Zheng ZY; Downstream Processing Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore, Singapore., Mahfut FB; Cell Line Development Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore, Singapore., Yang Y; Cell Line Development Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore, Singapore., Ogino M; Functional Materials Development, R&D Center, R&D and Engineering, Organo Corporation, Koto City, Japan., Okada K; Functional Materials Development, R&D Center, R&D and Engineering, Organo Corporation, Koto City, Japan., Sato K; Functional Materials Development, R&D Center, R&D and Engineering, Organo Corporation, Koto City, Japan., Zhang W; Downstream Processing Group, Bioprocessing Technology Institute, Agency for Science, Technology and Research, Singapore, Singapore.
المصدر: PloS one [PLoS One] 2023 Jan 25; Vol. 18 (1), pp. e0280760. Date of Electronic Publication: 2023 Jan 25 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Antibodies, Monoclonal* , Technology*, Cations
مستخلص: One of the key challenges in downstream bioprocessing is to obtain products of high purity in a productive fashion through the effective removal of process and product related impurities. While a classical simulated moving bed (SMB) system operation can typically achieve a 2-component separation between the weakly bound impurities and target species, here we present an advanced SMB approach that can achieve a 3-component separation, including the removal of the strongly bound impurities from the target species. As a proof-of-concept, we demonstrate the enhanced removal of strongly bound host cell proteins (HCP) from the target monoclonal antibody (mAb) through the utilisation of the advanced SMB approach in a non-affinity cation exchange (CEX) capture step. In this way, 1 less polishing step was required to achieve the therapeutic requirements of < 100 ppm HCP and the overall process recovery was increased by ~ 6% compared to the corresponding process that utilised a batch CEX operation. The non-affinity CEX capture platform technology established through the utilisation of the advanced SMB approach presented here can potentially be further applied to address the downstream processing challenges presented by other challenging biotherapeutic modalities to yield a final target product with improved purity and recovery.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Antibodies, Monoclonal)
0 (Cations)
تواريخ الأحداث: Date Created: 20230125 Date Completed: 20230127 Latest Revision: 20230317
رمز التحديث: 20230320
مُعرف محوري في PubMed: PMC9876269
DOI: 10.1371/journal.pone.0280760
PMID: 36696419
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0280760