دورية أكاديمية
أعرض في EDS

The effect of age and FSH stimulation on the ovarian follicular response, nuclear maturation, and gene expression of cumulus-oocyte complexes in prepubertal gilts.

التفاصيل البيبلوغرافية
العنوان: The effect of age and FSH stimulation on the ovarian follicular response, nuclear maturation, and gene expression of cumulus-oocyte complexes in prepubertal gilts.
المؤلفون: Alfradique VAP; Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil. Electronic address: vivianangelico@gmail.com., Alves SVP; Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil., Netto DLS; Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil., Machado AF; Departamento de Zootecnia, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil., Penitente-Filho JM; Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil., da Silva W; Departamento de Zootecnia, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil., Brandão FZ; Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil, 64, CEP 24230-340, Niterói, RJ, Brazil., Lopes MS; Topigs Norsvin Brasil, Rua Visconde do Rio Branco, 1310, CEP 80420-210, Curitiba, PR, Brazil., Guimarães SEF; Departamento de Zootecnia, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil. Electronic address: sfacioni@ufv.br.
المصدر: Theriogenology [Theriogenology] 2023 Mar 15; Vol. 199, pp. 57-68. Date of Electronic Publication: 2023 Jan 18.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE
أسماء مطبوعة: Publication: [New York, N.Y.?] : Elsevier
Original Publication: Los Altos, Calif., Geron-X.
مواضيع طبية MeSH: Ovarian Follicle*/physiology , Oocytes*/physiology, Swine ; Female ; Animals ; Ovary ; Sus scrofa ; Follicle Stimulating Hormone/pharmacology ; Follicle Stimulating Hormone/metabolism ; Gene Expression
مستخلص: This study investigated the effects of age and FSH treatment on the ovarian response, follicular fluid (FF) biochemical composition, nuclear maturation, and molecular profile of cumulus-oocytes complexes (COCs) recovered from prepubertal gilts. Thirty-five prepubertal gilts were separated according to age [140 (n = 20) or 160 (n = 15) days], and within each age, the gilts were allotted to receive either 100 mg of FSH [treated; G140+FSH (n = 10) and G160+FSH (n = 7)] or saline solution [control; G140+control (n = 10) and G160+control (n = 8)]. Thus, four experimental groups were included in this study. In the FSH-treated gilts, the percentage of medium follicles increased (P < 0.0001) in the same proportion with which the percentage of small follicles decreased (P < 0.0001). In addition, the glucose concentration in the FF obtained from medium follicles increased (P < 0.05), while that of triglycerides decreased (P < 0.05) in the FSH-treated gilts. The FSH stimulation also improved (P < 0.05) the number of grade I COCs obtained from medium follicles and the meiotic maturation and BCB + rates. FSH treatment only upregulated (P < 0.05) HMGCR expression in immature COCs from prepubertal gilts. The metaphase II and BCB + rates, FF glucose and plasma IGF-1 levels were greater (P < 0.05) in prepubertal gilts at 160 than at 140 days of age. Age had no effect (P > 0.05) on the transcript abundance of the target genes in immature COCs. Hence, oocytes obtained from 140-day-old prepubertal gilts appeared less meiotically competent than those of 160-day-old prepubertal gilts. Our study suggests a possible strategy of using FSH treatment to improve oocyte quantity, quality, and nuclear maturation in 140 and 160-day-old prepubertal gilts.
Competing Interests: Declaration of competing interest The authors have no conflict of interest to declare.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Follicular environment; Gonadotrophin; In vitro maturation; Maternal age; Oocyte; Ovarian stimulation; Pig; peri-pubertal; qPCR
المشرفين على المادة: 9002-68-0 (Follicle Stimulating Hormone)
تواريخ الأحداث: Date Created: 20230125 Date Completed: 20230222 Latest Revision: 20230222
رمز التحديث: 20240628
DOI: 10.1016/j.theriogenology.2023.01.015
PMID: 36696770
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3231
DOI:10.1016/j.theriogenology.2023.01.015