دورية أكاديمية
أعرض في EDS

Comparative Study of Ectopic Lymphoid Aggregates in Sheep and Murine Models of Bleomycin-Induced Pulmonary Fibrosis.

التفاصيل البيبلوغرافية
العنوان: Comparative Study of Ectopic Lymphoid Aggregates in Sheep and Murine Models of Bleomycin-Induced Pulmonary Fibrosis.
المؤلفون: Perera UE; School of Veterinary Science, The University of Melbourne, Parkville, VIC, Australia., Organ L; Nottingham Respiratory Research Unit, University of Nottingham, Nottingham, UK., Royce SG; Cardiovascular Disease Program, Monash Biomedicine Discovery Institute, Department of Pharmacology, Monash University, Clayton, Victoria, Australia., Samuel CS; Cardiovascular Disease Program, Monash Biomedicine Discovery Institute, Department of Pharmacology, Monash University, Clayton, Victoria, Australia., Derseh HB; School of Veterinary Science, The University of Melbourne, Parkville, VIC, Australia., Dewage SNV; Walter and Eliza Hall Institute of Medical Research, Parkville, Australia., Koumoundouros E; Department of Electrical and Electronic Engineering, The University of Melbourne, Parkville, VIC, Australia., Stent A; School of Veterinary Science, The University of Melbourne, Werribee, VIC, Australia., Snibson KJ; School of Veterinary Science, The University of Melbourne, Parkville, VIC, Australia.
المصدر: Canadian respiratory journal [Can Respir J] 2023 Jan 18; Vol. 2023, pp. 1522593. Date of Electronic Publication: 2023 Jan 18 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Hindawi Publishing Corporation Country of Publication: Egypt NLM ID: 9433332 Publication Model: eCollection Cited Medium: Internet ISSN: 1916-7245 (Electronic) Linking ISSN: 11982241 NLM ISO Abbreviation: Can Respir J Subsets: MEDLINE
أسماء مطبوعة: Publication: <2015- > : Cairo : Hindawi Publishing Corporation
Original Publication: Oakville, Ont. : Pulsus Group Inc., 1994-
مواضيع طبية MeSH: Bleomycin*/toxicity , Idiopathic Pulmonary Fibrosis*, Humans ; Mice ; Animals ; Disease Models, Animal ; Lung/pathology ; Inflammation
مستخلص: Idiopathic pulmonary fibrosis (IPF) is a chronic disease characterized by excessive deposition of extracellular matrix in the interstitial lung parenchyma, often manifested by dyspnea and progressive loss of lung function. The role of inflammation in the pathogenesis of IPF is not well understood. This study evaluated the histopathological and inflammatory components of bleomycin-induced pulmonary fibrosis in mouse and sheep models, in terms of their ability to translate to the human IPF. Merino sheep ( n  = 8) were bronchoscopically administered with two bleomycin infusions, two weeks apart, into a caudal lung segment, with a saline (control) administered into a caudal segment in the opposite lung. Balb/c mice were twice intranasally instilled, one week apart, with either bleomycin ( n  = 7); or saline (control, n  = 7). Lung samples were taken for the histopathological assessment 28 days in sheep and 21 days in mice after the first bleomycin administration. We observed tertiary lymphoid aggregates, in the fibrotic lung parenchyma of sheep, but not in mouse lung tissues exposed to bleomycin. B-cell and T-cell infiltration significantly increased in sheep lung tissues compared to mouse lung tissues due to bleomycin injury. Statistical analysis showed that the fibrotic score, fibrotic fraction, and tissue fraction significantly increased in sheep lung tissues compared to murine lung tissues. The presence of tertiary lymphoid aggregates in the lung parenchyma and increased infiltration of T-cells and B-cells, in the sheep model, may be useful for the future study of the underlying inflammatory disease mechanisms in the lung parenchyma of IPF patients.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2023 Udari Eshani Perera et al.)
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المشرفين على المادة: 11056-06-7 (Bleomycin)
تواريخ الأحداث: Date Created: 20230130 Date Completed: 20230131 Latest Revision: 20230203
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9876680
DOI: 10.1155/2023/1522593
PMID: 36710924
قاعدة البيانات: MEDLINE
الوصف
تدمد:1916-7245
DOI:10.1155/2023/1522593