دورية أكاديمية
Open questions in human lung organoid research.
| العنوان: | Open questions in human lung organoid research. |
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| المؤلفون: | Hughes T; Wellcome Trust/CRUK Gurdon Institute and Department Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom., Dijkstra KK; Department of Molecular Oncology and Immunology, The Netherlands Cancer Institute, Amsterdam, Netherlands.; Cancer Evolution and Genome Instability Laboratory, The Francis Crick Institute, London, United Kingdom., Rawlins EL; Wellcome Trust/CRUK Gurdon Institute and Department Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom., Hynds RE; Epithelial Cell Biology in ENT Research (EpiCENTR) Group, Developmental Biology and Cancer Department, Great Ormond Street UCL Institute of Child Health, University College London, London, United Kingdom.; CRUK Lung Cancer Centre of Excellence, UCL Cancer Institute, University College London, London, United Kingdom.; Cancer Evolution and Genome Instability Laboratory, The Francis Crick Institute, London, United Kingdom. |
| المصدر: | Frontiers in pharmacology [Front Pharmacol] 2023 Jan 13; Vol. 13, pp. 1083017. Date of Electronic Publication: 2023 Jan 13 (Print Publication: 2022). |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media] Country of Publication: Switzerland NLM ID: 101548923 Publication Model: eCollection Cited Medium: Print ISSN: 1663-9812 (Print) Linking ISSN: 16639812 NLM ISO Abbreviation: Front Pharmacol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Media] |
| مستخلص: | Organoids have become a prominent model system in pulmonary research. The ability to establish organoid cultures directly from patient tissue has expanded the repertoire of physiologically relevant preclinical model systems. In addition to their derivation from adult lung stem/progenitor cells, lung organoids can be derived from fetal tissue or induced pluripotent stem cells to fill a critical gap in modelling pulmonary development in vitro . Recent years have seen important progress in the characterisation and refinement of organoid culture systems. Here, we address several open questions in the field, including how closely organoids recapitulate the tissue of origin, how well organoids recapitulate patient cohorts, and how well organoids capture diversity within a patient. We advocate deeper characterisation of models using single cell technologies, generation of more diverse organoid biobanks and further standardisation of culture media. Competing Interests: KD provides consultancy services to Achilles Therapeutics. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Hughes, Dijkstra, Rawlins and Hynds.) |
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| معلومات مُعتمدة: | 20265 United Kingdom CRUK_ Cancer Research UK; 25253 United Kingdom CRUK_ Cancer Research UK; MR/P009581/1 United Kingdom MRC_ Medical Research Council; 24956 United Kingdom CRUK_ Cancer Research UK; 20466 United Kingdom CRUK_ Cancer Research UK; 17786 United Kingdom CRUK_ Cancer Research UK; 30025 United Kingdom CRUK_ Cancer Research UK; 209199/Z/17/Z United Kingdom WT_ Wellcome Trust; MR/R015635/1 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: 3D cell culture; epithelial cells; in vitro models; lung stem cells; respiratory biology |
| تواريخ الأحداث: | Date Created: 20230130 Latest Revision: 20240320 |
| رمز التحديث: | 20240320 |
| مُعرف محوري في PubMed: | PMC9880211 |
| DOI: | 10.3389/fphar.2022.1083017 |
| PMID: | 36712670 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1663-9812 |
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| DOI: | 10.3389/fphar.2022.1083017 |