دورية أكاديمية
أعرض في EDS

Salt-induced phosphoproteomic changes in the subfornical organ in rats with chronic kidney disease.

التفاصيل البيبلوغرافية
العنوان: Salt-induced phosphoproteomic changes in the subfornical organ in rats with chronic kidney disease.
المؤلفون: Wang X; National Clinical Research Center for Kidney Disease, State Key Laboratory of Organ Failure Research, Guangdong Provincial Clinical Research Center for Kidney Disease, Guangdong Provincial Key Laboratory of Renal Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou, China., Wang H; National Clinical Research Center for Kidney Disease, State Key Laboratory of Organ Failure Research, Guangdong Provincial Clinical Research Center for Kidney Disease, Guangdong Provincial Key Laboratory of Renal Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou, China., Li J; Nephrology Division, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China., Li L; The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China., Wang Y; Anshun People's Hospital of Guizhou Province, Anshun, China., Li A; National Clinical Research Center for Kidney Disease, State Key Laboratory of Organ Failure Research, Guangdong Provincial Clinical Research Center for Kidney Disease, Guangdong Provincial Key Laboratory of Renal Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou, China.
المصدر: Renal failure [Ren Fail] 2023 Dec; Vol. 45 (1), pp. 2171886.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Informa Healthcare Country of Publication: England NLM ID: 8701128 Publication Model: Print Cited Medium: Internet ISSN: 1525-6049 (Electronic) Linking ISSN: 0886022X NLM ISO Abbreviation: Ren Fail Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Informa Healthcare
Original Publication: New York, N.Y. : M. Dekker, c1987-
مواضيع طبية MeSH: Subfornical Organ*/physiology , Renal Insufficiency, Chronic*, Rats ; Animals ; Rats, Sprague-Dawley ; Chromatography, Liquid ; Phosphopeptides/pharmacology ; Tandem Mass Spectrometry ; Sodium Chloride, Dietary/pharmacology ; Phosphoproteins/metabolism ; Phosphoproteins/pharmacology
مستخلص: Objectives: Subfornical organ (SFO) is vital in chronic kidney disease (CKD) progression caused by high salt levels. The current study investigated the effects of high salt on phosphoproteomic changes in SFO in CKD rats.
Methods: 5/6 nephrectomized rats were fed a normal-salt diet (0.4%) (NC group) or a high-salt diet (4%) (HC group) for three weeks, while sham-operated rats were fed a normal-salt diet (0.4%) (NS group). For phosphoproteomic analysis of SFO in different groups, TiO 2 enrichment, isobaric tags for relative and absolute quantification (iTRAQ) labeling, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used.
Results: There were 6808 distinct phosphopeptides found, which corresponded to 2661 phosphoproteins. NC group had 168 upregulated and 250 downregulated phosphopeptides compared to NS group. Comparison to NC group, HC group had 154 upregulated and 124 downregulated phosphopeptides. Growth associated protein 43 (GAP43) and heat shock protein 27 (Hsp27) were significantly upregulated phosphoproteins and may protect against high-salt damage. Differential phosphoproteins with tight functional connection were synapse proteins and microtubule-associated proteins, implying that high-salt diet disrupted brain's structure and function. Furthermore, differential phosphoproteins in HC/NC comparison group were annotated to participate in GABAergic synapse signaling pathway and aldosterone synthesis and secretion, which attenuated inhibitory neurotransmitter effects and increased sympathetic nerve activity (SNA).
Discussion: This large scale phosphoproteomic profiling of SFO sheds light on how salt aggravates CKD via the central nervous system.
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فهرسة مساهمة: Keywords: High-salt diet; chronic kidney disease; phosphopeptides; subfornical organ
المشرفين على المادة: 0 (Phosphopeptides)
0 (Sodium Chloride, Dietary)
0 (Phosphoproteins)
تواريخ الأحداث: Date Created: 20230130 Date Completed: 20230131 Latest Revision: 20230202
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9888458
DOI: 10.1080/0886022X.2023.2171886
PMID: 36715439
قاعدة البيانات: MEDLINE
الوصف
تدمد:1525-6049
DOI:10.1080/0886022X.2023.2171886