دورية أكاديمية
Detection and Assessment of Wolbachia pipientis Infection.
| العنوان: | Detection and Assessment of Wolbachia pipientis Infection. |
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| المؤلفون: | Nevalainen LBM; Department of Biology, Indiana University, Bloomington, IN, USA., Newton ILG; Department of Biology, Indiana University, Bloomington, IN, USA. irnewton@indiana.edu. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2626, pp. 291-307. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Drosophila melanogaster*/genetics , Wolbachia*/genetics, Animals ; Multilocus Sequence Typing ; In Situ Hybridization, Fluorescence ; Drosophila/genetics |
| مستخلص: | Wolbachia pipientis is a widespread vertically transmitted intracellular bacterium naturally present in the model organism Drosophila melanogaster. As Wolbachia is present in a large number of Drosophila lines, it is critical for researchers to be able to identify which of their stocks maintain this infection to avoid any potential confounding variables. Here, we describe methods for detecting the bacterium and assessing the infection, including polymerase chain reaction (PCR) of DNA, multi-locus sequence typing (MLST) to identify strains, western blotting for protein detection, and immunohistochemistry and fluorescence in situ hybridization (FISH) of Drosophila ovaries to visually detect Wolbachia by fluorescence microscopy. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| معلومات مُعتمدة: | R01 AI144430 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Drosophila; Endosymbiont; FISH; Fluorescence microscopy; Immunolabeling; MLST; Microbiome; PCR; Western blotting; Wolbachia |
| SCR Organism: | Wolbachia pipientis |
| تواريخ الأحداث: | Date Created: 20230130 Date Completed: 20230201 Latest Revision: 20230330 |
| رمز التحديث: | 20230331 |
| مُعرف محوري في PubMed: | PMC10046185 |
| DOI: | 10.1007/978-1-0716-2970-3_15 |
| PMID: | 36715911 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-2970-3_15 |