دورية أكاديمية
The gut microbiome, short chain fatty acids, and related metabolites in cystic fibrosis patients with and without colonic adenomas.
| العنوان: | The gut microbiome, short chain fatty acids, and related metabolites in cystic fibrosis patients with and without colonic adenomas. |
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| المؤلفون: | Baldwin-Hunter BL; Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA., Rozenberg FD; Microbiome and Pathogen Genomics Collaborative Center, Columbia University Irving Medical Center, New York, NY, USA., Annavajhala MK; Microbiome and Pathogen Genomics Collaborative Center, Columbia University Irving Medical Center, New York, NY, USA., Park H; Microbiome and Pathogen Genomics Collaborative Center, Columbia University Irving Medical Center, New York, NY, USA., DiMango EA; Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA; Gunnar Esiason Adult Cystic Fibrosis and Lung Disease Program, Columbia University Irving Medical Center, New York, NY, USA., Keating CL; Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA; Gunnar Esiason Adult Cystic Fibrosis and Lung Disease Program, Columbia University Irving Medical Center, New York, NY, USA., Uhlemann AC; Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA; Microbiome and Pathogen Genomics Collaborative Center, Columbia University Irving Medical Center, New York, NY, USA; Digestive and Liver Disease Research Center, Columbia University Irving Medical Center, New York, NY, USA. Electronic address: au21102@cumc.columbia.edu., Abrams JA; Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA; Digestive and Liver Disease Research Center, Columbia University Irving Medical Center, New York, NY, USA. Electronic address: ja660@cumc.columbia.edu. |
| المصدر: | Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society [J Cyst Fibros] 2023 Jul; Vol. 22 (4), pp. 738-744. Date of Electronic Publication: 2023 Jan 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101128966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-5010 (Electronic) Linking ISSN: 15691993 NLM ISO Abbreviation: J Cyst Fibros Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c2002- |
| مواضيع طبية MeSH: | Cystic Fibrosis*/complications , Cystic Fibrosis*/microbiology , Gastrointestinal Microbiome* , Colonic Neoplasms*/diagnosis , Colonic Neoplasms*/etiology , Adenoma*/diagnosis, Adult ; Humans ; RNA, Ribosomal, 16S/genetics ; Fatty Acids, Volatile/metabolism ; Feces/microbiology |
| مستخلص: | Background: Adults with cystic fibrosis (CF) are at increased risk for colon cancer. CF patients have reductions in intestinal bacteria that produce short chain fatty acids (SCFAs), although it is unclear whether this corresponds with intestinal SCFA levels and the presence of colonic neoplasia. The aim of this study was to compare gut microbiome and SCFA composition in patients with and without CF, and to assess associations with colonic adenomas. Methods: Colonic aspirates were obtained from adults with and without CF undergoing colon cancer screening or surveillance colonoscopy. Microbiome characterization was performed by 16S rRNA V3-V4 sequencing. Targeted profiling of SCFAs and related metabolites was performed by LC-MS. Results: 42 patients (21 CF, 21 control) were enrolled. CF patients had significantly reduced alpha diversity and decreased relative abundance of many SCFA-producing taxa. There were no significant differences in SCFA levels in CF patients, although there were reduced levels of branched chain fatty acids (BCFAs) and related metabolites. CF patients with adenomas, but not controls with adenomas, had significantly increased relative abundance of Bacteroides fragilis. CF microbiome composition was significantly associated with isovalerate concentration and the presence of adenomas. Conclusions: CF patients have marked disturbances in the gut microbiome, and CF patients with adenomas had notably increased relative abundance of B. fragilis, a pathogen known to promote colon cancer. Reductions in BCFAs but not SCFAs were found in CF. Further studies are warranted to evaluate the role of B. fragilis as well the biological significance of reductions in BCFAs in CF. Competing Interests: Declaration of Competing Interest There are no conflicts of interest to report for all authors. (Copyright © 2023. Published by Elsevier B.V.) |
| معلومات مُعتمدة: | R01 AI116939 United States AI NIAID NIH HHS; TL1 TR001875 United States TR NCATS NIH HHS |
| فهرسة مساهمة: | Keywords: Branched chain fatty acids; Colorectal cancer; Dysbiosis; Host-microbe interactions; Metabolome |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) 0 (Fatty Acids, Volatile) |
| تواريخ الأحداث: | Date Created: 20230130 Date Completed: 20230911 Latest Revision: 20240202 |
| رمز التحديث: | 20240202 |
| DOI: | 10.1016/j.jcf.2023.01.013 |
| PMID: | 36717332 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-5010 |
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| DOI: | 10.1016/j.jcf.2023.01.013 |