دورية أكاديمية
High autophagic vesicle content marks facultative stem cells of the gut.
| العنوان: | High autophagic vesicle content marks facultative stem cells of the gut. |
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| المؤلفون: | Parham LR; Division of Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Johnson NM; Medical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA., Lengner CJ; Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Cell & Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA, USA., Hamilton KE; Division of Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA, USA. |
| المصدر: | Autophagy [Autophagy] 2023 Sep; Vol. 19 (9), pp. 2611-2612. Date of Electronic Publication: 2023 Feb 14. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101265188 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8635 (Electronic) Linking ISSN: 15548627 NLM ISO Abbreviation: Autophagy Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Georgetown, TX : Landes Bioscience, 2005- |
| مواضيع طبية MeSH: | Autophagy* , Stem Cells*, Animals ; Prospective Studies ; Intestinal Mucosa ; Cell Differentiation ; Intestines ; Mammals |
| مستخلص: | Understanding how macroautophagy/autophagy contributes to tissue homeostasis is essential for understanding organismal health. The intestinal epithelium is an ideal model to define mechanisms that regulate tissue homeostasis because it houses well-defined populations of intestinal stem cells. Active intestinal stem cells (a-ISCs) are defined by their active cycling and self-renewal during homeostasis, which supports continual tissue turnover in vivo. In vitro, this is observed as long-term organoid formation capacity. A second population of stem cells, called "facultative intestinal stem cells" (f-ISCs), are defined by their ability to 1) survive tissue damage that depletes the injury-sensitive a-ISCs and 2) reenter the cell cycle to repopulate the a-ISC compartment and regenerate the epithelium. The prospective identification of f-ISCs has been challenging, as cells expressing markers of multiple differentiated lineages, particularly secretory lineages, appear to function as f-ISCs in diverse injury contexts. We evaluated cell age (defined as time elapsed after cell cycle exit) and autophagic state (marked by autophagic vesicle content) as molecular features that may be related to f-ISC capacity. We found that autophagic state, but not cell age, prospectively identifies f-ISCs within multiple lineages. As such, we describe autophagy as a lineage-agnostic marker of f-ISC capacity in the mammalian intestine. |
| معلومات مُعتمدة: | F31 AI150224 United States AI NIAID NIH HHS; P30 DK050306 United States DK NIDDK NIH HHS; R01 DK124369 United States DK NIDDK NIH HHS; R01 DK106309 United States DK NIDDK NIH HHS; F31 DK124956 United States DK NIDDK NIH HHS |
| فهرسة مساهمة: | Keywords: Autophagy; facultative intestinal stem cell; intestinal regeneration; organoid formation; paligenosis |
| تواريخ الأحداث: | Date Created: 20230201 Date Completed: 20230801 Latest Revision: 20240216 |
| رمز التحديث: | 20240216 |
| مُعرف محوري في PubMed: | PMC10392747 |
| DOI: | 10.1080/15548627.2023.2174297 |
| PMID: | 36722667 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1554-8635 |
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| DOI: | 10.1080/15548627.2023.2174297 |