Toxoplasma gondii SAG2, SAG3 and GRA6 alleles and single nucleotide polymorphism in congenital infections with known parasite load and clinical outcome.

التفاصيل البيبلوغرافية
العنوان:	Toxoplasma gondii SAG2, SAG3 and GRA6 alleles and single nucleotide polymorphism in congenital infections with known parasite load and clinical outcome.
المؤلفون:	Targa LS; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Soroepidemiologia e Imunobiologia, São Paulo, São Paulo, Brazil., Santos EHD; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Soroepidemiologia e Imunobiologia, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Departamento de Pediatria, São Paulo, São Paulo, Brazil., Yamamoto L; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Soroepidemiologia e Imunobiologia, São Paulo, São Paulo, Brazil., Barreira GA; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Soroepidemiologia e Imunobiologia, São Paulo, São Paulo, Brazil.; Faculdade Israelita de Ciências da Saúde Albert Einstein, São Paulo, São Paulo, Brazil., Rodrigues KA; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Soroepidemiologia e Imunobiologia, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Departamento de Pediatria, São Paulo, São Paulo, Brazil., Rocha MC; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Soroepidemiologia e Imunobiologia, São Paulo, São Paulo, Brazil., Kanunfre KA; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Soroepidemiologia e Imunobiologia, São Paulo, São Paulo, Brazil., Okay TS; Universidade de São Paulo, Faculdade de Medicina, Instituto de Medicina Tropical de São Paulo, Laboratório de Soroepidemiologia e Imunobiologia, São Paulo, São Paulo, Brazil.; Universidade de São Paulo, Faculdade de Medicina, Departamento de Pediatria, São Paulo, São Paulo, Brazil.
المصدر:	Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2023 Jan 30; Vol. 65, pp. e8. Date of Electronic Publication: 2023 Jan 30 (Print Publication: 2023).
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo Country of Publication: Brazil NLM ID: 7507484 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-9946 (Electronic) Linking ISSN: 00364665 NLM ISO Abbreviation: Rev Inst Med Trop Sao Paulo Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Sao Paulo : Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo
مواضيع طبية MeSH:	Fetal Diseases/parasitology , Toxoplasma/genetics , Toxoplasmosis*/parasitology, Female ; Humans ; Infant ; Alleles ; Amniotic Fluid/parasitology ; Asymptomatic Infections ; Parasite Load ; Polymorphism, Single Nucleotide ; Protozoan Proteins/genetics ; Pregnancy
مستخلص:	Amniotic fluid DNA samples were genotyped by multilocus-nested-PCR-RFLP, but only three of 11 markers amplified 113 of 122 (92.6%) samples, resulting in 12 untyped and 101 partial non-archetypal genotypes. The 101 typed samples were subdivided into four groups: G1 with 73 samples (5'and 3' SAG2 allele I + SAG3 allele III + GRA6 allele III), 53 had parasite load ≤ 102 parasites/mL (43 asymptomatic, 10 mild infections), 17 had load > 102 and ≤ 103 (one mild, 13 moderate and three severe), and three had load > 103 parasites/mL (three severe); G2 with 22 samples (5'and 3' SAG2 allele I + SAG3 allele III), all parasite load levels ≤ 102 parasites/mL (18 asymptomatic and four mild); G3 with five samples (5' and 3' SAG2 allele I + SAG3 allele II), parasite load ≤ 102 parasites/mL (three asymptomatic and two mild); G4 with one sample (5' and 3' SAG2 allele II + SAG3 allele II + GRA6 allele I), a parasite load < 102 parasites/mL in an asymptomatic infant. After DNA sequencing, restriction sites confirmed SAG2, SAG3 and GRA6 alleles in 98.7%, 100% and 100% of the cases, respectively, while single nucleotide polymorphisms confirmed 90% of 5'-SAG2 allele I; 98.7% of 3'-SAG2 allele I; 98% of SAG-3 allele III, but only 40% of GRA6 allele III results. For the moment, partial non-archetypal genotypes of parasites did not show any relationship with either parasite load in amniotic fluid samples or clinical outcome of infants at the age of 12 months.
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المشرفين على المادة:	0 (Protozoan Proteins)
تواريخ الأحداث:	Date Created: 20230201 Date Completed: 20230203 Latest Revision: 20230204
رمز التحديث:	20231215
مُعرف محوري في PubMed:	PMC9886222
DOI:	10.1590/S1678-9946202365008
PMID:	36722670
قاعدة البيانات:	MEDLINE

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تدمد:	1678-9946
DOI:	10.1590/S1678-9946202365008