Effects of discontinuous blue light stimulation on the electrophysiological properties of neurons lacking opsin expression in vitro: Implications for optogenetic experiments.

التفاصيل البيبلوغرافية
العنوان:	Effects of discontinuous blue light stimulation on the electrophysiological properties of neurons lacking opsin expression in vitro: Implications for optogenetic experiments.
المؤلفون:	Lightning A; Université Claude Bernard Lyon 1, CNRS, INSERM, Centre de Recherche en Neurosciences de Lyon CRNL U1028UMR5292, NEUROPOP, Bron, France., Bourzeix M; Aix Marseille Univ, CNRS UMR 7289, Institut de Neurosciences de la Timone (INT), Marseille, France., Beurrier C; Aix Marseille Univ, CNRS UMR 7289, Institut de Neurosciences de la Timone (INT), Marseille, France., Kuczewski N; Université Claude Bernard Lyon 1, CNRS, INSERM, Centre de Recherche en Neurosciences de Lyon CRNL U1028UMR5292, NEUROPOP, Bron, France.
المصدر:	The European journal of neuroscience [Eur J Neurosci] 2023 Mar; Vol. 57 (6), pp. 885-899. Date of Electronic Publication: 2023 Feb 10.
نوع المنشور:	Journal Article; Research Support, Non-U.S. Gov't
اللغة:	English
بيانات الدورية:	Publisher: Wiley-Blackwell Country of Publication: France NLM ID: 8918110 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-9568 (Electronic) Linking ISSN: 0953816X NLM ISO Abbreviation: Eur J Neurosci Subsets: MEDLINE
أسماء مطبوعة:	Publication: : Oxford : Wiley-Blackwell Original Publication: Oxford, UK : Published on behalf of the European Neuroscience Association by Oxford University Press, c1989-
مواضيع طبية MeSH:	Opsins/genetics , Opsins/metabolism , Optogenetics*/methods, Neurons/physiology ; Electrophysiological Phenomena ; Rod Opsins
مستخلص:	Neuronal sensitivity to light stimulation can be a significant confounding factor for assays that use light to study neuronal processes, such as optogenetics and fluorescent imaging. While continuous one-photon (1P) blue light stimulation has been shown to be responsible for a decrease in firing activity in several neuronal subtypes, discontinuous 1P blue light stimulation commonly used in optogenetic experiments is supposed to have a negligible action. In the present report, we tested experimentally this theoretical prediction by assessing the effects produced by the most commonly used patterns of discontinuous 1P light stimulation on several electrophysiological parameters in brain slices. We found that, compared with continuous stimulation, the artefactual effect of light is reduced when discontinuous stimulation is used, especially when the duty cycle and light power are low. (© 2023 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.)
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فهرسة مساهمة:	Keywords: heat production; neurons; optogenetics; patch-clamp
المشرفين على المادة:	0 (Opsins) 0 (Rod Opsins)
تواريخ الأحداث:	Date Created: 20230202 Date Completed: 20230320 Latest Revision: 20230327
رمز التحديث:	20231215
DOI:	10.1111/ejn.15927
PMID:	36726326
قاعدة البيانات:	MEDLINE

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تدمد:	1460-9568
DOI:	10.1111/ejn.15927