دورية أكاديمية
[Identification of the target site of antimicrobial peptide AMP-17 against Candida albicans ].
العنوان: | [Identification of the target site of antimicrobial peptide AMP-17 against Candida albicans ]. |
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المؤلفون: | Yang L; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China.; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou, China., Tian Z; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China., Zhou L; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China.; School of Public Health, Guizhou Medical University, Guiyang 550025, Guizhou, China., Sun C; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China., Huang M; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China., Tian C; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China., Peng J; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China.; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou, China., Guo G; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China.; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou, China.; Translational Medicine Research Center, Guizhou Medical University, Guiyang 550025, Guizhou, China. |
المصدر: | Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2023 Jan 25; Vol. 39 (1), pp. 304-317. |
نوع المنشور: | English Abstract; Journal Article |
اللغة: | Chinese |
بيانات الدورية: | Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9426463 Publication Model: Print Cited Medium: Internet ISSN: 1872-2075 (Electronic) Linking ISSN: 10003061 NLM ISO Abbreviation: Sheng Wu Gong Cheng Xue Bao Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Beijing : Ke xue chu ban she, |
مواضيع طبية MeSH: | Candida albicans* , Antimicrobial Peptides*, Humans ; Proteomics ; Peptides/pharmacology ; Transcription Factors/metabolism ; Antifungal Agents/pharmacology |
مستخلص: | Candida albicans is one of the major causes of invasive fungal infections and a serious opportunistic pathogen in immunocompromised individuals. The antimicrobial peptide AMP-17 has prominent anti- Candida activity, and proteomic analysis revealed significant differences in the expression of cell wall ( XOG1 ) and oxidative stress ( SRR1 ) genes upon the action of AMP-17 on C . albicans , suggesting that AMP-17 may exert anti- C . albicans effects by affecting the expression of XOG1 and SRR1 genes. To further investigate whether XOG1 and SRR1 genes were the targets of AMP-17, C . albicans xog1Δ/Δ and srr1Δ/Δ mutants were constructed using the clustered regulatory interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9) system. Phenotypic observations revealed that deletion of two genes had no significant effect on C . albicans growth and biofilm formation, whereas XOG1 gene deletion affected in vitro stress response and mycelium formation of C . albicans . Drug sensitivity assay showed that the MIC |
فهرسة مساهمة: | Keywords: AMP-17; Candida albicans; SRR1; XOG1; antimicrobial peptide; clustered regulatory interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9) |
المشرفين على المادة: | 0 (Antimicrobial Peptides) 0 (Peptides) 0 (Transcription Factors) 0 (Antifungal Agents) |
تواريخ الأحداث: | Date Created: 20230204 Date Completed: 20230207 Latest Revision: 20230207 |
رمز التحديث: | 20240628 |
DOI: | 10.13345/j.cjb.220499 |
PMID: | 36738218 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1872-2075 |
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DOI: | 10.13345/j.cjb.220499 |