[Identification of the target site of antimicrobial peptide AMP-17 against Candida albicans ].

التفاصيل البيبلوغرافية
العنوان:	[Identification of the target site of antimicrobial peptide AMP-17 against Candida albicans ].
المؤلفون:	Yang L; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China.; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou, China., Tian Z; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China., Zhou L; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China.; School of Public Health, Guizhou Medical University, Guiyang 550025, Guizhou, China., Sun C; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China., Huang M; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China., Tian C; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China., Peng J; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China.; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou, China., Guo G; The Key and Characteristic Laboratory of Modern Pathogen Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, China.; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou, China.; Translational Medicine Research Center, Guizhou Medical University, Guiyang 550025, Guizhou, China.
المصدر:	Sheng wu gong cheng xue bao = Chinese journal of biotechnology [Sheng Wu Gong Cheng Xue Bao] 2023 Jan 25; Vol. 39 (1), pp. 304-317.
نوع المنشور:	English Abstract; Journal Article
اللغة:	Chinese
بيانات الدورية:	Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9426463 Publication Model: Print Cited Medium: Internet ISSN: 1872-2075 (Electronic) Linking ISSN: 10003061 NLM ISO Abbreviation: Sheng Wu Gong Cheng Xue Bao Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Beijing : Ke xue chu ban she,
مواضيع طبية MeSH:	Candida albicans* , Antimicrobial Peptides*, Humans ; Proteomics ; Peptides/pharmacology ; Transcription Factors/metabolism ; Antifungal Agents/pharmacology
مستخلص:	Candida albicans is one of the major causes of invasive fungal infections and a serious opportunistic pathogen in immunocompromised individuals. The antimicrobial peptide AMP-17 has prominent anti- Candida activity, and proteomic analysis revealed significant differences in the expression of cell wall ( XOG1 ) and oxidative stress ( SRR1 ) genes upon the action of AMP-17 on C . albicans , suggesting that AMP-17 may exert anti- C . albicans effects by affecting the expression of XOG1 and SRR1 genes. To further investigate whether XOG1 and SRR1 genes were the targets of AMP-17, C . albicans xog1Δ/Δ and srr1Δ/Δ mutants were constructed using the clustered regulatory interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9) system. Phenotypic observations revealed that deletion of two genes had no significant effect on C . albicans growth and biofilm formation, whereas XOG1 gene deletion affected in vitro stress response and mycelium formation of C . albicans . Drug sensitivity assay showed that the MIC 80 values of AMP-17 against xog1Δ/Δ and srr1Δ/Δ mutants increased from 8 μg/mL (for the wild type C . albicans SC5314) to 16 μg/mL, while the MIC 80 values against srr1Δ/Δ : : srr1 revertants decreased to the level of the wild type SC5314. In addition, the ability of AMP-17 to inhibit biofilm formation of both deletion strains was significantly reduced compared to that of wild type SC5314, indicating that the susceptibility of the deletion mutants to AMP-17 was reduced in both the yeast state and during biofilm formation. These results suggest that XOG1 and SRR1 genes are likely two of the potential targets for AMP-17 to exert anti -C . albicans effects, which may facilitate further exploration of the antibacterial mechanism of novel peptide antifungal drugs.
فهرسة مساهمة:	Keywords: AMP-17; Candida albicans; SRR1; XOG1; antimicrobial peptide; clustered regulatory interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9)
المشرفين على المادة:	0 (Antimicrobial Peptides) 0 (Peptides) 0 (Transcription Factors) 0 (Antifungal Agents)
تواريخ الأحداث:	Date Created: 20230204 Date Completed: 20230207 Latest Revision: 20230207
رمز التحديث:	20240628
DOI:	10.13345/j.cjb.220499
PMID:	36738218
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1872-2075
DOI:	10.13345/j.cjb.220499