دورية أكاديمية
In-depth quantitative proteomics analysis revealed C1GALT1 depletion in ECC-1 cells mimics an aggressive endometrial cancer phenotype observed in cancer patients with low C1GALT1 expression.
العنوان: | In-depth quantitative proteomics analysis revealed C1GALT1 depletion in ECC-1 cells mimics an aggressive endometrial cancer phenotype observed in cancer patients with low C1GALT1 expression. |
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المؤلفون: | Montero-Calle A; Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, 28220, Majadahonda, Madrid, Spain., López-Janeiro Á; Department of Pathology, Hospital Universitario La Paz, 28046, Madrid, Spain., Mendes ML; Department of Infection and Immunity, Luxembourg Institute of Health, 1445, Strassen, Luxembourg., Perez-Hernandez D; Department of Infection and Immunity, Luxembourg Institute of Health, 1445, Strassen, Luxembourg., Echevarría I; Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, 28220, Majadahonda, Madrid, Spain.; Biochemistry and Molecular Biology Department, Facultad de Óptica y Optometría, Universidad Complutense de Madrid, 28037, Madrid, Spain., Ruz-Caracuel I; Department of Pathology, Hospital Universitario La Paz, 28046, Madrid, Spain., Heredia-Soto V; Translational Oncology, La Paz University Hospital (IdiPAZ), 28046, Madrid, Spain.; Center for Biomedical Research in the Cancer Network (Centro de Investigación Biomédica en Red de Cáncer, CIBERONC), Instituto de Salud Carlos III, 28046, Madrid, Spain., Mendiola M; Center for Biomedical Research in the Cancer Network (Centro de Investigación Biomédica en Red de Cáncer, CIBERONC), Instituto de Salud Carlos III, 28046, Madrid, Spain.; Molecular Pathology and Therapeutic Targets Group, La Paz University Hospital (IdiPAZ), 28046, Madrid, Spain., Hardisson D; Department of Pathology, Hospital Universitario La Paz, 28046, Madrid, Spain.; Center for Biomedical Research in the Cancer Network (Centro de Investigación Biomédica en Red de Cáncer, CIBERONC), Instituto de Salud Carlos III, 28046, Madrid, Spain.; Molecular Pathology and Therapeutic Targets Group, La Paz University Hospital (IdiPAZ), 28046, Madrid, Spain.; Faculty of Medicine, Universidad Autónoma de Madrid, 28029, Madrid, Spain., Argüeso P; Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts, USA., Peláez-García A; Molecular Pathology and Therapeutic Targets Group, La Paz University Hospital (IdiPAZ), 28046, Madrid, Spain. alberto.pelaez@idipaz.es., Guzman-Aranguez A; Biochemistry and Molecular Biology Department, Facultad de Óptica y Optometría, Universidad Complutense de Madrid, 28037, Madrid, Spain. aguzman@opt.ucm.es., Barderas R; Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, 28220, Majadahonda, Madrid, Spain. r.barderasm@isciii.es.; Functional Proteomics Unit, UFIEC, Instituto de Salud Carlos III, 28220, Majadahonda, Madrid, Spain. r.barderasm@isciii.es. |
المصدر: | Cellular oncology (Dordrecht) [Cell Oncol (Dordr)] 2023 Jun; Vol. 46 (3), pp. 697-715. Date of Electronic Publication: 2023 Feb 06. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 101552938 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-3436 (Electronic) Linking ISSN: 22113428 NLM ISO Abbreviation: Cell Oncol (Dordr) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Dordrecht : Springer |
مواضيع طبية MeSH: | Proteomics* , Endometrial Neoplasms*, Humans ; Female ; Glycosylation ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Phenotype ; Galactosyltransferases |
مستخلص: | Background: Endometrial cancer (EC) is the most common cancer of the female reproductive organs. Despite the good overall prognosis of most low-grade ECs, FIGO I and FIGO II patients might experience tumor recurrence and worse prognosis. The study of alterations related to EC pathogenesis might help to get insights into underlying mechanisms involved in EC development and progression. Methods: Core tumoral samples were used to investigate the role of C1GALT1 in EC by immunohistochemistry (IHC). ECC-1 cells were used as endometrioid EC model to investigate the effect of C1GALT1 depletion using C1GALT1 specific shRNAs. SILAC quantitative proteomics analyses and cell-based assays, PCR, qPCR, WB, dot-blot and IHC analyses were used to identify, quantify and validate dysregulation of proteins. Results: Low C1GALT1 protein expression levels associate to a more aggressive phenotype of EC. Out of 5208 proteins identified and quantified by LC-MS/MS, 100 proteins showed dysregulation (log Conclusion: C1GALT1 stably depleted ECC-1 cells mimic an EC aggressive phenotype observed in patients and might be useful for the identification and validation of EC markers of progression. (© 2023. The Author(s).) |
References: | Acta Med Litu. 2019;26(3):181-190. (PMID: 32015673) J Natl Cancer Inst. 1975 Feb;54(2):335-9. (PMID: 163330) J Exp Med. 2020 Jan 6;217(1):. (PMID: 31645367) Biol Reprod. 2006 Sep;75(3):387-94. (PMID: 16707768) J Comput Assist Tomogr. 2020 Sep/Oct;44(5):714-729. (PMID: 32842057) Future Oncol. 2013 Nov;9(11):1773-93. (PMID: 24156336) Cells. 2021 Mar 15;10(3):. (PMID: 33804148) Chin Med J (Engl). 2019 Jan 20;132(2):161-170. (PMID: 30614867) J Immunol. 2013 Mar 15;190(6):2896-903. (PMID: 23401595) Cell Biosci. 2021 Aug 26;11(1):166. (PMID: 34452648) Biomedicines. 2022 Feb 01;10(2):. (PMID: 35203553) J Vis Exp. 2014 Oct 27;(92):e51998. (PMID: 25408172) Glycobiology. 2010 Aug;20(8):931-49. (PMID: 20368670) J Cancer. 2021 Mar 5;12(9):2550-2559. (PMID: 33854616) Oncotarget. 2016 Jun 7;7(23):35478-89. (PMID: 27007155) Med Oncol. 2013 Mar;30(1):438. (PMID: 23315219) J Bioenerg Biomembr. 2018 Jun;50(3):199-204. (PMID: 29127647) Cancer Cell Int. 2022 Jan 6;22(1):7. (PMID: 34991599) Cancers (Basel). 2019 Nov 22;11(12):. (PMID: 31766622) Signal Transduct Target Ther. 2021 Mar 6;6(1):111. (PMID: 33677469) Mol Cell Proteomics. 2015 Feb;14(2):303-15. (PMID: 25505127) Br J Cancer. 2022 Jun;126(11):1604-1615. (PMID: 35347323) Exp Hematol Oncol. 2021 Mar 31;10(1):25. (PMID: 33789749) Int J Gynecol Pathol. 2010 Nov;29(6):555-61. (PMID: 20881856) Gynecol Oncol. 2005 Mar;96(3):601-9. (PMID: 15721400) PLoS One. 2014 Mar 18;9(3):e92142. (PMID: 24642596) J Exp Med. 2009 Oct 26;206(11):2309-12. (PMID: 19841088) Proc Natl Acad Sci U S A. 2014 Sep 30;111(39):E4066-75. (PMID: 25118277) Obstet Gynecol. 2012 Aug;120(2 Pt 1):383-97. (PMID: 22825101) Oncogene. 2016 Feb 18;35(7):919-28. (PMID: 25982276) Nucleic Acids Res. 2007 Jul;35(Web Server issue):W169-75. (PMID: 17576678) Am J Cancer Res. 2017 May 01;7(5):1188-1197. (PMID: 28560066) PLoS One. 2013 May 16;8(5):e63695. (PMID: 23696849) EBioMedicine. 2020 Mar;53:102701. (PMID: 32151798) Gastroenterology. 2018 Nov;155(5):1608-1624. (PMID: 30086262) Front Oncol. 2021 Jun 25;11:646217. (PMID: 34249684) Genome Biol. 2003;4(5):P3. (PMID: 12734009) Cell Death Discov. 2021 Oct 11;7(1):284. (PMID: 34667158) APMIS. 2009 Oct;117(10):693-707. (PMID: 19775337) J Biol Chem. 2009 Aug 21;284(34):23037-45. (PMID: 19556244) Virchows Arch. 2021 Dec;479(6):1167-1176. (PMID: 34420090) Int J Mol Sci. 2021 Dec 07;22(24):. (PMID: 34947980) Proteomics. 2015 Sep;15(18):3175-92. (PMID: 26097186) Cancer Cell. 2007 Jun;11(6):498-512. (PMID: 17560332) Semin Oncol Nurs. 2019 Apr;35(2):157-165. (PMID: 30867105) Front Mol Biosci. 2021 Dec 02;8:714906. (PMID: 34926571) Cancer Res. 1991 Oct 15;51(20):5661-6. (PMID: 1913685) Diabetes. 2022 Mar 1;71(3):497-510. (PMID: 35040477) Clin Sci (Lond). 2016 Feb;130(4):205-20. (PMID: 26769657) Front Cell Dev Biol. 2021 Nov 29;9:777182. (PMID: 34912807) Cells. 2022 Jan 27;11(3):. (PMID: 35159257) In Vivo. 2020 Jan-Feb;34(1):177-184. (PMID: 31882477) Front Oncol. 2022 Jul 25;12:903033. (PMID: 35957902) Expert Rev Clin Immunol. 2022 Jan;18(1):67-74. (PMID: 34928185) Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613. (PMID: 30476243) Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4581-7. (PMID: 19407012) Anticancer Res. 2017 Jul;37(7):3905-3910. (PMID: 28668893) HLA. 2016 Dec;88(6):275-286. (PMID: 27679419) Anal Chem. 2015 Apr 21;87(8):4110-6. (PMID: 25837572) Pathol Res Pract. 2017 Apr;213(4):348-352. (PMID: 28215640) Nat Rev Cancer. 2015 Sep;15(9):540-55. (PMID: 26289314) BMC Bioinformatics. 2013 Jan 16;14:7. (PMID: 23323831) Cell. 2009 Jun 12;137(6):1124-35. (PMID: 19524514) Cells. 2021 Jan 08;10(1):. (PMID: 33430152) Int J Gynaecol Obstet. 2021 Oct;155 Suppl 1:45-60. (PMID: 34669196) Cell Immunol. 2018 Nov;333:46-57. (PMID: 29576316) Cancer Med. 2018 Oct;7(10):5205-5216. (PMID: 30270520) Front Cell Dev Biol. 2021 Jul 07;9:707970. (PMID: 34307388) Mol Biol Cell. 2005 Feb;16(2):927-42. (PMID: 15574878) Int J Gynecol Pathol. 2019 Jan;38 Suppl 1:S64-S74. (PMID: 30550484) Histopathology. 2012 Apr;60(5):826-37. (PMID: 22348356) Mol Cell Proteomics. 2013 Jun;12(6):1602-20. (PMID: 23443137) Cell Rep Med. 2021 Jun 15;2(6):100318. (PMID: 34195683) Ginekol Pol. 2012 Jan;83(1):22-6. (PMID: 22384635) Oncogene. 2013 Jan 24;32(4):403-13. (PMID: 22430211) Nucleic Acids Res. 2018 Jan 4;46(D1):D956-D963. (PMID: 29136207) Nature. 2020 Dec;588(7839):693-698. (PMID: 33177715) Oncogenesis. 2022 Feb 15;11(1):8. (PMID: 35169131) Cells. 2020 Feb 14;9(2):. (PMID: 32075174) Biomolecules. 2021 Nov 24;11(12):. (PMID: 34944403) Mol Biol Rep. 2021 Jul;48(7):5699-5705. (PMID: 34241773) Proteomics. 2017 Oct;17(19):. (PMID: 28861940) Lancet. 2005 Aug 6-12;366(9484):491-505. (PMID: 16084259) |
معلومات مُعتمدة: | FPU predoctoral contract to A.M-C. Ministerio de Educación, Cultura y Deporte; PI17/01723 AES-ISCIII; PI20CIII/00019 AES-ISCIII |
فهرسة مساهمة: | Keywords: C1GALT1; Endometrial cancer; O-glycosylation; Quantitative proteomics; SILAC |
المشرفين على المادة: | EC 2.4.1.- (C1GALT1 protein, human) EC 2.4.1.- (Galactosyltransferases) |
تواريخ الأحداث: | Date Created: 20230206 Date Completed: 20230525 Latest Revision: 20230526 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC10205863 |
DOI: | 10.1007/s13402-023-00778-w |
PMID: | 36745330 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2211-3436 |
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DOI: | 10.1007/s13402-023-00778-w |