التفاصيل البيبلوغرافية
| العنوان: |
Gallionellaceae pangenomic analysis reveals insight into phylogeny, metabolic flexibility, and iron oxidation mechanisms. |
| المؤلفون: |
Hoover RL; Microbiology Graduate Program, University of Delaware, Newark, Delaware, USA.; Department of Earth Sciences, University of Delaware, Newark, Delaware, USA., Keffer JL; Department of Earth Sciences, University of Delaware, Newark, Delaware, USA., Polson SW; Department of Computer and Information Sciences, University of Delaware, Newark, Delaware, USA.; Center for Bioinformatics and Computational Biology, University of Delaware, Newark, Delaware, USA., Chan CS; Microbiology Graduate Program, University of Delaware, Newark, Delaware, USA.; Department of Earth Sciences, University of Delaware, Newark, Delaware, USA.; School of Marine Science and Policy, University of Delaware, Newark, Delaware, USA. |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Feb 08. Date of Electronic Publication: 2023 Feb 08. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
The iron-oxidizing Gallionellaceae drive a wide variety of biogeochemical cycles through their metabolisms and biominerals. To better understand the environmental impacts of Gallionellaceae, we need to improve our knowledge of their diversity and metabolisms, especially any novel iron oxidation mechanisms. Here, we used a pangenomic analysis of 103 genomes to resolve Gallionellaceae phylogeny and explore the range of genomic potential. Using a concatenated ribosomal protein tree and key gene patterns, we determined Gallionellaceae has four genera, divided into two groups-iron-oxidizing bacteria (FeOB) Gallionella , Sideroxydans , and Ferriphaselus with known iron oxidases (Cyc2, MtoA) and nitrite-oxidizing bacteria (NOB) Candidatus Nitrotoga with nitrite oxidase (Nxr). The FeOB and NOB have similar electron transport chains, including genes for reverse electron transport and carbon fixation. Auxiliary energy metabolisms including S oxidation, denitrification, and organotrophy were scattered throughout the Gallionellaceae FeOB. Within FeOB, we found genes that may represent adaptations for iron oxidation, including a variety of extracellular electron uptake (EEU) mechanisms. FeOB genomes encoded more predicted c -type cytochromes overall, notably more multiheme c -type cytochromes (MHCs) with >10 CXXCH motifs. These include homologs of several predicted outer membrane porin-MHC complexes, including MtoAB and Uet. MHCs are known to efficiently conduct electrons across longer distances and function across a wide range of redox potentials that overlap with mineral redox potentials, which can help expand the range of usable iron substrates. Overall, the results of pangenome analyses suggest that the Gallionellaceae genera Gallionella , Sideroxydans , and Ferriphaselus are primarily iron oxidizers, capable of oxidizing dissolved Fe 2+ as well as a range of solid iron or other mineral substrates.
Competing Interests: We declare that we have no conflicts of interest. |
| التعليقات: |
Update in: mSystems. 2023 Oct 26;:e0003823. (PMID: 37882557) |
| معلومات مُعتمدة: |
P20 GM103446 United States GM NIGMS NIH HHS |
| تواريخ الأحداث: |
Date Created: 20230207 Latest Revision: 20231221 |
| رمز التحديث: |
20231221 |
| مُعرف محوري في PubMed: |
PMC9900912 |
| DOI: |
10.1101/2023.01.26.525709 |
| PMID: |
36747706 |
| قاعدة البيانات: |
MEDLINE |
