دورية أكاديمية
أعرض في EDS

Development and evaluation of a multiplex serodiagnostic bead assay (BurkPx) for accurate melioidosis diagnosis.

التفاصيل البيبلوغرافية
العنوان: Development and evaluation of a multiplex serodiagnostic bead assay (BurkPx) for accurate melioidosis diagnosis.
المؤلفون: Settles EW; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America.; Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, United States of America., Sonderegger D; Department of Mathematics and Statistics, Northern Arizona University, Flagstaff, Arizona, United States of America., Shannon AB; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Celona KR; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Lederer R; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Yi J; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Seavey C; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Headley K; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Mbegbu M; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Harvey M; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Keener M; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Allender C; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Hornstra H; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Monroy FP; Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, United States of America., Woerle C; Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia., Theobald V; Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia., Mayo M; Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia., Currie BJ; Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.; Infectious Diseases Department and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia., Keim P; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America.; Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, United States of America.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2023 Feb 08; Vol. 17 (2), pp. e0011072. Date of Electronic Publication: 2023 Feb 08 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Melioidosis*/microbiology , Burkholderia pseudomallei*, Humans ; Antibodies, Bacterial ; Antigens, Bacterial ; Sensitivity and Specificity
مستخلص: Burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative soil bacterium well recognized in Southeast Asia and northern Australia. However, wider and expanding global distribution of B. pseudomallei has been elucidated. Early diagnosis is critical for commencing the specific therapy required to optimize outcome. Serological testing using the indirect hemagglutination (IHA) antibody assay has long been used to augment diagnosis of melioidosis and to monitor progress. However, cross reactivity and prior exposure may complicate the diagnosis of current clinical disease (melioidosis). The goal of our study was to develop and initially evaluate a serology assay (BurkPx) that capitalized upon host response to multiple antigens. Antigens were selected from previous studies for expression/purification and conjugation to microspheres for multiantigen analysis. Selected serum samples from non-melioidosis controls and serial samples from culture-confirmed melioidosis patients were used to characterize the diagnostic power of individual and combined antigens at two times post admission. Multiple variable models were developed to evaluate multivariate antigen reactivity, identify important antigens, and determine sensitivity and specificity for the diagnosis of melioidosis. The final multiplex assay had a diagnostic sensitivity of 90% and specificity of 93%, which was superior to any single antigen in side-by-side comparisons. The sensitivity of the assay started at >85% for the initial serum sample after admission and increased to 94% 21 days later. Weighting antigen contribution to each model indicated that certain antigen contributed to diagnosis more than others, which suggests that the number of antigens in the assay can be decreased. In summation, the BurkPx assay can facilitate the diagnosis of melioidosis and potentially improve on currently available serology assays. Further evaluation is now required in both melioidosis-endemic and non-endemic settings.
Competing Interests: The authors have no competing interests.
(Copyright: © 2023 Settles et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Antibodies, Bacterial)
0 (Antigens, Bacterial)
تواريخ الأحداث: Date Created: 20230208 Date Completed: 20230210 Latest Revision: 20230227
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9907819
DOI: 10.1371/journal.pntd.0011072
PMID: 36753506
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0011072