دورية أكاديمية
أعرض في EDS

NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates.

التفاصيل البيبلوغرافية
العنوان: NHP BurkPx: A multiplex serodiagnostic bead assay to monitor Burkholderia pseudomallei exposures in non-human primates.
المؤلفون: Celona KR; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Shannon AB; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Sonderegger D; Department of Mathematics and Statistics, Northern Arizona University, Flagstaff, Arizona, United States of America., Yi J; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Monroy FP; Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, United States of America., Allender C; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Hornstra H; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America., Barnes MB; Tulane National Primate Research Center, Tulane University, Covington, Louisiana, United States of America., Didier ES; Tulane National Primate Research Center, Tulane University, Covington, Louisiana, United States of America., Bohm RP; Tulane National Primate Research Center, Tulane University, Covington, Louisiana, United States of America., Phillippi-Falkenstein K; Tulane National Primate Research Center, Tulane University, Covington, Louisiana, United States of America., Sanford D; Battelle Memorial Institute, Columbus, Ohio, United States of America., Keim P; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America.; Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, United States of America., Settles EW; Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America.; Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona, United States of America.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2023 Feb 08; Vol. 17 (2), pp. e0011067. Date of Electronic Publication: 2023 Feb 08 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Burkholderia pseudomallei* , Melioidosis*/diagnosis , Melioidosis*/veterinary , Melioidosis*/epidemiology, Animals ; Humans ; Antibodies, Bacterial ; Antigens, Bacterial ; Primates
مستخلص: Background: Melioidosis is a disease caused by the bacterium Burkholderia pseudomallei, infecting humans and non-human primates (NHP) through contaminated soil or water. World-wide there are an estimated 165,000 human melioidosis cases each year, but recordings of NHP cases are sporadic. Clinical detection of melioidosis in humans is primarily by culturing B. pseudomallei, and there are no standardized detection protocols for NHP. NHP are an important animal model for melioidosis research including clinical trials and development of biodefense countermeasures.
Methodology/principle Findings: We evaluated the diagnostic potential of the multiple antigen serological assay, BurkPx, in NHP using two sera sets: (i) 115 B. pseudomallei-challenged serum samples from 80 NHP collected each week post-exposure (n = 52) and at euthanasia (n = 47), and (ii) 126 B. pseudomallei-naïve/negative serum samples. We observed early IgM antibody responses to carbohydrate antigens followed by IgG antibody recognition to multiple B. pseudomallei protein antigens during the second week of infection. B. pseudomallei negative serum samples had low to intermediate antibody cross reactivity to the antigens in this assay. Infection time was predicted as the determining factor in the variation of antibody responses, with 77.67% of variation explained by the first component of the principal component analysis. A multiple antigen model generated a binary prediction metric ([Formula: see text]), which when applied to all data resulted in 100% specificity and 63.48% sensitivity. Removal of week 1 B. pseudomallei challenged serum samples increased the sensitivity of the model to 95%.
Conclusion/significance: We employed a previously standardized assay for humans, the BurkPx assay, and assessed its diagnostic potential for detection of B. pseudomallei exposure in NHP. The assay is expected to be useful for surveillance in NHP colonies, in investigations of suspected accidental releases or exposures, and for identifying vaccine correlates of protection.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Celona et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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معلومات مُعتمدة: P51 OD011104 United States OD NIH HHS; U42 OD010568 United States OD NIH HHS; U42 OD024282 United States OD NIH HHS
المشرفين على المادة: 0 (Antibodies, Bacterial)
0 (Antigens, Bacterial)
تواريخ الأحداث: Date Created: 20230208 Date Completed: 20230210 Latest Revision: 20240207
رمز التحديث: 20240207
مُعرف محوري في PubMed: PMC9907805
DOI: 10.1371/journal.pntd.0011067
PMID: 36753522
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0011067