دورية أكاديمية
Differentiated mouse kidney tubuloids as a novel in vitro model to study collecting duct physiology.
| العنوان: | Differentiated mouse kidney tubuloids as a novel in vitro model to study collecting duct physiology. |
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| المؤلفون: | Olde Hanhof CJA; Department of Molecular Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands., Dilmen E; Department of Molecular Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands., Yousef Yengej FA; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences, Utrecht, Netherlands.; Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, Netherlands., Latta F; Department of Molecular Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands., Ammerlaan CME; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences, Utrecht, Netherlands.; Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, Netherlands., Schreurs J; Department of Molecular Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands., Hooijmaijers L; Department of Molecular Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands., Jansen J; Department of Pathology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands.; Department of Pediatric Nephrology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Amalia Children's Hospital, Nijmegen, Netherlands.; Institute of Experimental Medicine and Systems Biology, Medical Faculty RWTH Aachen University, Aachen, Germany., Rookmaaker MB; Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, Netherlands., Orhon I; Department of Molecular Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands., Verhaar MC; Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, Netherlands., Hoenderop JG; Department of Molecular Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, Netherlands. |
| المصدر: | Frontiers in cell and developmental biology [Front Cell Dev Biol] 2023 Jan 25; Vol. 11, pp. 1086823. Date of Electronic Publication: 2023 Jan 25 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101630250 Publication Model: eCollection Cited Medium: Print ISSN: 2296-634X (Print) Linking ISSN: 2296634X NLM ISO Abbreviation: Front Cell Dev Biol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2013]- |
| مستخلص: | Kidney tubuloids are cell models that are derived from human or mouse renal epithelial cells and show high similarities with their in vivo counterparts. Tubuloids grow polarized in 3D, allow for long-term expansion, and represent multiple segments of the nephron, as shown by their gene expression pattern. In addition, human tubuloids form tight, functional barriers and have been succesfully used for drug testing. Our knowledge of mouse tubuloids, on the other hand, is only minimal. In this study, we further characterized mouse tubuloids and differentiated them towards the collecting duct, which led to a significant upregulation of collecting duct-specific mRNAs of genes and protein expression, including the water channel AQP2 and the sodium channel ENaC. Differentiation resulted in polarized expression of collecting duct water channels AQP2 and AQP3. Also, a physiological response to desmopressin and forskolin stimulation by translocation of AQP2 to the apical membrane was demonstrated. Furthermore, amiloride-sensitive ENaC-mediated sodium uptake was shown in differentiated tubuloids using radioactive tracer sodium. This study demonstrates that mouse tubuloids can be differentiated towards the collecting duct and exhibit collecting duct-specific function. This illustrates the potential use of mouse kidney tubuloids as novel in vitro models to study (patho)physiology of kidney diseases. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Olde Hanhof, Dilmen, Yousef Yengej, Latta, Ammerlaan, Schreurs, Hooijmaijers, Jansen, Rookmaaker, Orhon, Verhaar and Hoenderop.) |
| References: | Curr Mol Pharmacol. 2013 Mar;6(1):28-34. (PMID: 23547932) J Hepatol. 2014 Oct;61(4):925-43. (PMID: 24911463) Pflugers Arch. 2012 Dec;464(6):601-11. (PMID: 23014881) Am J Physiol Renal Physiol. 2015 Jun 1;308(11):F1306-15. (PMID: 25810438) Cancer Sci. 2005 Nov;96(11):791-800. (PMID: 16271073) PLoS One. 2013 Jun 14;8(6):e66750. (PMID: 23799132) Kidney Res Clin Pract. 2019 Jun 30;38(2):145-158. (PMID: 31189221) Clin J Am Soc Nephrol. 2021 Apr 7;16(4):620-630. (PMID: 32238367) Nat Rev Nephrol. 2021 Nov;17(11):765-781. (PMID: 34211154) Ann Med. 2006;38(3):200-11. (PMID: 16720434) Am J Physiol Renal Physiol. 2003 May;284(5):F893-901. (PMID: 12676734) Dev Biol. 2006 Mar 15;291(2):325-39. (PMID: 16442091) Toxicol Appl Pharmacol. 2014 Sep 15;279(3):409-418. (PMID: 25036895) Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6269-73. (PMID: 7517548) Integr Biol (Camb). 2011 Feb;3(2):134-41. (PMID: 21079870) J Am Soc Nephrol. 2013 Nov;24(11):1793-805. (PMID: 24029424) Nat Biotechnol. 2019 Mar;37(3):303-313. (PMID: 30833775) Exp Cell Res. 2017 Sep 1;358(1):39-44. (PMID: 28372972) Front Mol Biosci. 2020 Mar 06;7:33. (PMID: 32211418) J Pharmacol Sci. 2016 Apr;130(4):219-25. (PMID: 26976493) Nat Commun. 2020 Mar 11;11(1):1310. (PMID: 32161258) Bioinformatics. 2009 Jun 1;25(11):1463-5. (PMID: 19346324) Am J Physiol Cell Physiol. 2020 Jan 1;318(1):C150-C162. (PMID: 31721612) J Biol Chem. 2003 Jan 10;278(2):1101-7. (PMID: 12374804) Am J Physiol. 1998 Nov;275(5):F796-801. (PMID: 9815137) Cell. 2016 Jun 16;165(7):1586-1597. (PMID: 27315476) Front Endocrinol (Lausanne). 2021 Jun 10;12:686818. (PMID: 34177810) Nano Lett. 2021 Jul 14;21(13):5850-5858. (PMID: 34156251) Am J Physiol Renal Physiol. 2010 Dec;299(6):F1320-7. (PMID: 20861081) Nature. 2015 Oct 22;526(7574):564-8. (PMID: 26444236) Am J Hypertens. 2020 Jul 18;33(7):670-675. (PMID: 32161960) Kidney Int. 1988 Dec;34(6):749-60. (PMID: 2850394) J Biol Chem. 2003 Dec 19;278(51):51213-22. (PMID: 14532287) PLoS One. 2012;7(2):e32843. (PMID: 22403603) J Am Soc Nephrol. 2006 May;17(5):1429-36. (PMID: 16611713) Cell Stem Cell. 2018 Dec 6;23(6):869-881.e8. (PMID: 30449713) Am J Physiol Renal Physiol. 2020 May 1;318(5):F1113-F1121. (PMID: 32174140) Am J Physiol. 1995 Jun;268(6 Pt 1):C1546-51. (PMID: 7541941) Genes Dis. 2018 Apr 23;5(2):137-149. (PMID: 30258943) Am J Physiol Renal Physiol. 2007 Aug;293(2):F476-85. (PMID: 17475898) Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1013-7. (PMID: 7532304) J Biol Chem. 2002 Mar 22;277(12):10379-86. (PMID: 11782489) Cells. 2020 Jan 13;9(1):. (PMID: 31941155) Cell Mol Life Sci. 2022 Apr 10;79(5):235. (PMID: 35397686) Proc Natl Acad Sci U S A. 1981 Jun;78(6):3363-7. (PMID: 6267587) Am J Physiol Renal Physiol. 2022 Oct 1;323(4):F479-F491. (PMID: 35979965) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) Genome Med. 2019 Jan 23;11(1):3. (PMID: 30674341) J Am Soc Nephrol. 2021 Apr;32(4):897-912. (PMID: 33769951) Cells. 2020 Sep 26;9(10):. (PMID: 32993088) Nature. 1993 Feb 11;361(6412):549-52. (PMID: 8429910) Am J Physiol Renal Physiol. 2017 Apr 1;312(4):F682-F688. (PMID: 28365586) J Clin Invest. 1999 Oct;104(7):R19-23. (PMID: 10510339) Tissue Eng Part C Methods. 2021 Mar;27(3):177-182. (PMID: 33544041) Pediatr Nephrol. 1995 Apr;9(2):228-34. (PMID: 7540850) Am J Physiol Renal Physiol. 2007 Jul;293(1):F178-85. (PMID: 17389677) Nat Biotechnol. 2022 Aug 29;:. (PMID: 36038632) Pediatr Clin North Am. 2019 Feb;66(1):227-234. (PMID: 30454745) J Pharm Sci. 2014 Apr;103(4):1298-304. (PMID: 24532159) Integr Blood Press Control. 2019 Sep 03;12:13-22. (PMID: 31564964) PLoS One. 2015 Mar 24;10(3):e0120132. (PMID: 25803827) Am J Physiol Renal Physiol. 2016 Dec 1;311(6):F1318-F1328. (PMID: 27760771) Nat Protoc. 2021 Apr;16(4):2023-2050. (PMID: 33674788) Nat Biotechnol. 2007 Jun;25(6):681-6. (PMID: 17529971) J Am Soc Nephrol. 1999 Dec;10(12):2527-33. (PMID: 10589691) Am J Physiol. 1995 Dec;269(6 Pt 2):F775-85. (PMID: 8594871) Front Physiol. 2022 May 24;13:816959. (PMID: 35685285) J Am Soc Nephrol. 2008 Oct;19(10):1845-54. (PMID: 18753254) Nature. 1994 Feb 3;367(6462):463-7. (PMID: 8107805) Cells Tissues Organs. 2006;184(1):16-22. (PMID: 17190976) Am J Physiol Renal Physiol. 2002 Dec;283(6):F1351-64. (PMID: 12426236) FEBS Lett. 1995 Mar 27;362(1):19-23. (PMID: 7698346) Am J Physiol Renal Physiol. 2006 Jul;291(1):F236-45. (PMID: 16467129) Pflugers Arch. 2022 Feb;474(2):217-229. (PMID: 34870751) Am J Physiol Renal Physiol. 2017 Apr 1;312(4):F744-F747. (PMID: 28179252) Am J Physiol Renal Physiol. 2017 Dec 1;313(6):F1254-F1263. (PMID: 28877883) Am J Physiol Renal Physiol. 2021 Oct 1;321(4):F480-F493. (PMID: 34423678) Science. 1994 Apr 1;264(5155):92-5. (PMID: 8140421) |
| فهرسة مساهمة: | Keywords: cell physiology; collecting duct; epithelial sodium transport; organoid; tubuloid; tubulopathy |
| تواريخ الأحداث: | Date Created: 20230210 Latest Revision: 20230211 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9905633 |
| DOI: | 10.3389/fcell.2023.1086823 |
| PMID: | 36760360 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2296-634X |
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| DOI: | 10.3389/fcell.2023.1086823 |