دورية أكاديمية
Clinical, histopathological, and molecular findings for Mycobacterium avium subspecies paratuberculosis (MAP) in dairy goats under semiarid conditions.
| العنوان: | Clinical, histopathological, and molecular findings for Mycobacterium avium subspecies paratuberculosis (MAP) in dairy goats under semiarid conditions. |
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| المؤلفون: | Roberto JPL; Federal University of Campina Grande, Post-Graduate Program in Animal Science and Health, Patos, PB, Brazil., Limeira CH; Federal University of Campina Grande, Post-Graduate Program in Animal Science and Health, Patos, PB, Brazil., Araújo Júnior JP; São Paulo of University, São Paulo, Brazil., Malossi CD; São Paulo of University, São Paulo, Brazil., Ullmann LS; São Paulo of University, São Paulo, Brazil., Silva MLCR; Federal University of Campina Grande, Post-Graduate Program in Animal Science and Health, Patos, PB, Brazil., Dantas AFM; Federal University of Campina Grande, Post-Graduate Program in Animal Science and Health, Patos, PB, Brazil., do Nascimento MJR; Federal University of Campina Grande, Post-Graduate Program in Animal Science and Health, Patos, PB, Brazil., de Azevedo SS; Federal University of Campina Grande, Post-Graduate Program in Animal Science and Health, Patos, PB, Brazil., Alves CJ; Federal University of Campina Grande, Post-Graduate Program in Animal Science and Health, Patos, PB, Brazil. Electronic address: clebertja@uol.com.br. |
| المصدر: | Tuberculosis (Edinburgh, Scotland) [Tuberculosis (Edinb)] 2023 Mar; Vol. 139, pp. 102319. Date of Electronic Publication: 2023 Feb 03. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Churchill Livingstone Country of Publication: Scotland NLM ID: 100971555 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-281X (Electronic) Linking ISSN: 14729792 NLM ISO Abbreviation: Tuberculosis (Edinb) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Edinburgh, New York : Churchill Livingstone, c2001- |
| مواضيع طبية MeSH: | Mycobacterium avium subsp. paratuberculosis*/genetics , Paratuberculosis*/diagnosis , Paratuberculosis*/epidemiology , Paratuberculosis*/microbiology , Mycobacterium tuberculosis*, Animals ; Female ; Lactation ; Goats |
| مستخلص: | The objective of this study was to report the occurrence of Mycobacterium avium subspecies paratuberculosis (MAP) in dairy goats, via description of their clinical presentation, histopathological findings, and molecular identification of the infectious agent. Screening was performed using IS900 real-time PCR (qPCR) in milk samples from 179 properties in the semiarid of Northeast region of Brazil. Pooled milk samples from all lactating goats from processing plants were submitted to molecular diagnosis. One property had a positive result at qPCR. The production unit which had the positive sample for MAP was located, and an on-site visit to this property was performed to collect individual milk samples, seven of which tested MAP positive by IS900 qPCR. With permission from the owner, two goats (Animal 1 was positive and Animal 2 was negative on first qPCR for MAP) were acquired and euthanized. Animals 1 and 2 had milk and portions of the duodenum, ileum, colon, and mesenteric lymph nodes positive at qPCR for MAP. Animal 1 also had MAP DNA detected in part of the jejunum and cecum. In animal 2, the ileocecal valve tested positive. MAP was not detected in the blood or feces of either animal; however; it was confirmed for the association of clinical findings, histopathology, and qPCR. The gene IS900 from the positive samples were sequenced and showed a 99% similarity with MAP. The MAP was identified for the first time in the goat milk and tissues in the semiarid region of Northeast Brazil. Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Goats; MAP; Milk; Production units; Semiarid |
| تواريخ الأحداث: | Date Created: 20230210 Date Completed: 20230314 Latest Revision: 20230408 |
| رمز التحديث: | 20230410 |
| DOI: | 10.1016/j.tube.2023.102319 |
| PMID: | 36764074 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-281X |
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| DOI: | 10.1016/j.tube.2023.102319 |