دورية أكاديمية
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Molecular Cloning and Characterization of a Serotonin N -Acetyltransferase Gene, xoSNAT3 , from Xanthomonas oryzae pv. oryzae .

التفاصيل البيبلوغرافية
العنوان: Molecular Cloning and Characterization of a Serotonin N -Acetyltransferase Gene, xoSNAT3 , from Xanthomonas oryzae pv. oryzae .
المؤلفون: Chen X; State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection, Ministry of Agriculture and Rural Affairs, Zhejiang Provincial Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China., Zhao Y; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China., Laborda P; School of Life Sciences, Nantong University, Nantong 226001, China., Yang Y; State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection, Ministry of Agriculture and Rural Affairs, Zhejiang Provincial Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China., Liu F; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
المصدر: International journal of environmental research and public health [Int J Environ Res Public Health] 2023 Jan 19; Vol. 20 (3). Date of Electronic Publication: 2023 Jan 19.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101238455 Publication Model: Electronic Cited Medium: Internet ISSN: 1660-4601 (Electronic) Linking ISSN: 16604601 NLM ISO Abbreviation: Int J Environ Res Public Health Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI, c2004-
مواضيع طبية MeSH: Arylalkylamine N-Acetyltransferase*/genetics , Arylalkylamine N-Acetyltransferase*/metabolism , Oryza*/microbiology, Animals ; Sheep ; Cloning, Molecular ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Virulence ; Plant Diseases/microbiology
مستخلص: Rice bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae ( Xoo ), is one of the top ten bacterial plant diseases worldwide. Serotonin N -acetyltransferase (SNAT) is one of the key rate-limiting enzymes in melatonin (MT) biosynthesis. However, its function in pathogenic bacteria remains unclear. In this study, a Xoo SNAT protein (xoSNAT3) that showed 27.39% homology with sheep SNAT was identified from a collection of 24 members of GCN5-related N -acetyltransferase (GNAT) superfamily in Xoo . This xoSNAT3 could be induced by MT. In tobacco-based transient expression system, xoSNAT3 was found localized on mitochondria. In vitro studies indicated that xoSNAT3 showed the optima enzymatic activity at 50 °C. The recombinant enzyme showed K m and V max values of 709.98 μM and 2.21 nmol/min/mg protein, respectively. Mutant △ xoSNAT3 showed greater impaired MT biosynthesis than the wild-type strain. Additionally, △ xoSNAT3 showed 14.06% less virulence and 26.07% less biofilm formation. Collectively, our results indicated that xoSNAT3 services as a SNAT involved in MT biosynthesis and pathogenicity in Xoo .
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فهرسة مساهمة: Keywords: Oryza sativa; Xoo; melatonin biosynthesis; serotonin N-acetyltransferase; xoSNAT3
المشرفين على المادة: EC 2.3.1.87 (Arylalkylamine N-Acetyltransferase)
0 (Bacterial Proteins)
SCR Organism: Xanthomonas oryzae
تواريخ الأحداث: Date Created: 20230211 Date Completed: 20230214 Latest Revision: 20230318
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9914633
DOI: 10.3390/ijerph20031865
PMID: 36767232
قاعدة البيانات: MEDLINE
الوصف
تدمد:1660-4601
DOI:10.3390/ijerph20031865