دورية أكاديمية
أعرض في EDS

Role of Histone Tails and Single Strand DNA Breaks in Nucleosomal Arrest of RNA Polymerase.

التفاصيل البيبلوغرافية
العنوان: Role of Histone Tails and Single Strand DNA Breaks in Nucleosomal Arrest of RNA Polymerase.
المؤلفون: Gerasimova NS; Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, Russia.; Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia., Pestov NA; Department of Pharmacology, Rutgers Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA., Studitsky VM; Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.; Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Jan 24; Vol. 24 (3). Date of Electronic Publication: 2023 Jan 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Nucleosomes*/genetics , Histones*/genetics , Histones*/metabolism, Transcription, Genetic ; RNA Polymerase II/genetics ; DNA Breaks, Single-Stranded ; DNA-Directed RNA Polymerases/genetics ; DNA-Directed RNA Polymerases/metabolism ; DNA/genetics ; DNA, Single-Stranded
مستخلص: Transcription through nucleosomes by RNA polymerases (RNAP) is accompanied by formation of small intranucleosomal DNA loops (i-loops). The i-loops form more efficiently in the presence of single-strand breaks or gaps in a non-template DNA strand (NT-SSBs) and induce arrest of transcribing RNAP, thus allowing detection of NT-SSBs by the enzyme. Here we examined the role of histone tails and extranucleosomal NT-SSBs in i-loop formation and arrest of RNAP during transcription of promoter-proximal region of nucleosomal DNA. NT-SSBs present in linker DNA induce arrest of RNAP +1 to +15 bp in the nucleosome, suggesting formation of the i-loops; the arrest is more efficient in the presence of the histone tails. Consistently, DNA footprinting reveals formation of an i-loop after stalling RNAP at the position +2 and backtracking to position +1. The data suggest that histone tails and NT-SSBs present in linker DNA strongly facilitate formation of the i-loops during transcription through the promoter-proximal region of nucleosomal DNA.
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معلومات مُعتمدة: RO1 GM119398 United States NH NIH HHS; 21-64-00001 Russian Science Foundation
فهرسة مساهمة: Keywords: DNA damage; DNA loop; chromatin structure; nucleosome; single-strand DNA breaks; transcription-coupled DNA repair
المشرفين على المادة: 0 (Nucleosomes)
0 (Histones)
EC 2.7.7.- (RNA Polymerase II)
EC 2.7.7.6 (DNA-Directed RNA Polymerases)
9007-49-2 (DNA)
0 (DNA, Single-Stranded)
تواريخ الأحداث: Date Created: 20230211 Date Completed: 20230214 Latest Revision: 20230214
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9917218
DOI: 10.3390/ijms24032295
PMID: 36768621
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms24032295