دورية أكاديمية
أعرض في EDS

The hybrid protein BTH2 suppresses allergic airway inflammation in a murine model of HDM-specific immunotherapy.

التفاصيل البيبلوغرافية
العنوان: The hybrid protein BTH2 suppresses allergic airway inflammation in a murine model of HDM-specific immunotherapy.
المؤلفون: da Silva ES; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Post-Graduate Program in Biotechnology of the Northeast Biotechnology Network (RENORBIO), Maceió, Brazil., de Santana MBR; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Silveira EF; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Torres RT; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Silva RC; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Fernandes AMS; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Belitardo EMMA; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Gonçalo Moniz Institute, Oswaldo Cruz Foundation (FioCruz), Salvador, Brazil., Garcés LFS; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Faculty of Health Sciences, Technical University of Ambato, Ambato, Ecuador., Santiago LF; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Urrego JR; Department of Pharmacy, University of Cartagena, Cartagena, Colombia., Vilas-Bôas DS; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Laboratory of Histotechnology, Department of Biomorphology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., de Freitas LAR; Gonçalo Moniz Institute, Oswaldo Cruz Foundation (FioCruz), Salvador, Brazil.; Department of Pathology of the School of Medicine, Federal University of Bahia, Salvador, Brazil., Zakzuk J; Institute of Immunological Research, University of Cartagena, Cartagena, Colombia., Pacheco LGC; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Cruz ÁA; ProAR Foundation and Federal University of Bahia, Salvador, Brazil., Ferreira F; Department of Biosciences, Paris-Lodron University of Salzburg, Salzburg, Austria., Cooper P; Institute of Infection and Immunity, St George's University of London, London, UK.; School of Medicine, International University of Ecuador, Quito, Ecuador., Caraballo L; Institute of Immunological Research, University of Cartagena, Cartagena, Colombia., Pinheiro CDS; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Alcantara-Neves NM; Laboratory of Allergology and Acarology (LAA), Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.; Post-Graduate Program in Biotechnology of the Northeast Biotechnology Network (RENORBIO), Maceió, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
المصدر: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology [Clin Exp Allergy] 2023 Aug; Vol. 53 (8), pp. 821-832. Date of Electronic Publication: 2023 Feb 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8906443 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2222 (Electronic) Linking ISSN: 09547894 NLM ISO Abbreviation: Clin Exp Allergy Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford : Blackwell Scientific Publications, c1989-
مواضيع طبية MeSH: Hypersensitivity*/therapy, Humans ; Mice ; Animals ; Disease Models, Animal ; Allergens ; Inflammation ; Cytokines ; Desensitization, Immunologic ; Immunoglobulin E
مستخلص: Background: Allergen-specific immunotherapy (AIT) is the only disease-modifying treatment approach to change disease-causing allergens. Hypoallergenic derivatives show promise as potential therapeutics, amongst which BTH2 was designed to induce tolerance against Blomia tropicalis allergy. Our aim was to investigate the hypoallergenicity and immunoregulatory activity of BTH2 in vitro and its therapeutic potential in a mouse model of AIT.
Methods: Recombinant Blo t 5 and Blo t 21 allergens and their hybrid derivatives (BTH1 and BTH2) were expressed and purified. IgE binding capacity was tested by ELISA using sera from Brazilian, Colombian, and Ecuadorian subjects. Secretion of cytokines in supernatants from human cell cultures was measured following stimulation with the four recombinants and controls. The capacity of BTH2 to ameliorate allergic airway inflammation induced by B. tropicalis extract was evaluated in a murine model of AIT.
Results: rBlo t 5 and rBlo t 21 were identified as major allergens in Latin American patients, and BTH2 had the lowest IgE binding. In vitro stimulation of human cells induced greater levels of IL-10 and IFN-γ and reduced the secretion of Th2 cytokines. BTH2 ameliorated allergic airway inflammation in B. tropicalis-challenged A/J mice, as evidenced by the histopathological and humoral biomarkers: decreased Th2 cytokines and cellular infiltration (especially eosinophils), lower activity of eosinophil peroxidase, an increase in IgG blocking antibodies and strong reduction of mucus production by goblet cells.
Conclusions: Our study shows that BTH2 represents a promising candidate for the treatment of B. tropicalis allergy with hypoallergenic, immune regulatory and therapeutic properties. Further pre-clinical studies are required in murine models of chronic asthma to further address the efficacy and safety of BTH2 as a vaccine against B. tropicalis-induced allergy.
(© 2023 John Wiley & Sons Ltd.)
التعليقات: Comment in: Clin Exp Allergy. 2023 Aug;53(8):788-790. (PMID: 37559562)
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فهرسة مساهمة: Keywords: Blomia tropicalis; allergen immunotherapy; experimental model; peripheral blood mononuclear cell; vaccine candidate
المشرفين على المادة: 0 (Allergens)
0 (Cytokines)
37341-29-0 (Immunoglobulin E)
تواريخ الأحداث: Date Created: 20230213 Date Completed: 20230811 Latest Revision: 20230824
رمز التحديث: 20231215
DOI: 10.1111/cea.14293
PMID: 36779555
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2222
DOI:10.1111/cea.14293