دورية أكاديمية
Evaluation of the role of clathrin and bacterial viability in the endocytosis of Lawsonia intracellularis .
| العنوان: | Evaluation of the role of clathrin and bacterial viability in the endocytosis of Lawsonia intracellularis . |
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| المؤلفون: | Pereira CER; Department of Clinic and Surgery, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States., Resende TP; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States., Daniel AGS; Department of Clinic and Surgery, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States., Vannucci FA; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States., Gebhart C; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States., Guedes RMC; Department of Clinic and Surgery, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. |
| المصدر: | Frontiers in veterinary science [Front Vet Sci] 2023 Jan 30; Vol. 10, pp. 1005676. Date of Electronic Publication: 2023 Jan 30 (Print Publication: 2023). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media S.A., [2014]- |
| مستخلص: | Lawsonia intracellularis is an obligate intracellular bacterium and causative agent of proliferative enteropathy. The pathogenesis of L. intracellularis is not completely understood, including the endocytic mechanisms to access the host cell cytoplasm. In this study, we evaluated the mechanisms involved in endocytosis of L. intracellularis in vitro using intestinal porcine epithelial cells (IPEC-J2). Confocal microscopy was used to co-localize L. intracellularis and clathrin. Clathrin gene knockdown was then applied to verify whether L. intracellularis endocytosis is clathrin-dependent. Finally, internalization of viable and non-viable (bacteria were inactivated by heat) L. intracellularis organisms were assessed to study the role of the host cell during bacterial endocytosis. L. intracellularis organisms were observed co-localized with clathrin by confocal microscopy but the amount of L. intracellularis internalized in cells, with and without clathrin knockdown, did not differ statistically. The internalization of non-viable L. intracellularis showed a decrease in the internalization in cells with less clathrin synthesis (P<0.05). The present study is the first to elucidate the involvement of clathrin in the endocytosis of L. intracellularis . Clathrin-mediated endocytosis was shown to be an important, but not required, process for L. intracellularis internalization in porcine intestinal epithelial cells. Independence of bacterial viability for host cell internalization was also confirmed. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Pereira, Resende, Daniel, Vannucci, Gebhart and Guedes.) |
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| فهرسة مساهمة: | Keywords: endocytosis; infection; intracellular bacteria; pathogenesis; proliferative enteropathy |
| تواريخ الأحداث: | Date Created: 20230216 Latest Revision: 20230217 |
| رمز التحديث: | 20230217 |
| مُعرف محوري في PubMed: | PMC9923121 |
| DOI: | 10.3389/fvets.2023.1005676 |
| PMID: | 36793380 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2297-1769 |
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| DOI: | 10.3389/fvets.2023.1005676 |