دورية أكاديمية
Graded Atmospheres of Volatile Pyrethroid Overlaid on Host Cues Can Be Established and Quantified Within a Novel Flight Chamber for Mosquito Behavior Studies.
| العنوان: | Graded Atmospheres of Volatile Pyrethroid Overlaid on Host Cues Can Be Established and Quantified Within a Novel Flight Chamber for Mosquito Behavior Studies. |
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| المؤلفون: | Seavey CE; University of Central Florida, College of Medicine, Department of Internal Medicine, FL 32827, USA., Doshi M; University of Central Florida, College of Medicine, Department of Internal Medicine, FL 32827, USA., Colamarino A; University of Central Florida, College of Medicine, Department of Internal Medicine, FL 32827, USA., Kim BN; University of Central Florida, College of Engineering and Computer Science, Department of Electrical and Computer Engineering, FL 32816, USA., Dickerson AK; Tickle College of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, TN 37996, USA., Willenberg BJ; University of Central Florida, College of Medicine, Department of Internal Medicine, FL 32827, USA. |
| المصدر: | Environmental entomology [Environ Entomol] 2023 Apr 18; Vol. 52 (2), pp. 197-209. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 7502320 Publication Model: Print Cited Medium: Internet ISSN: 1938-2936 (Electronic) Linking ISSN: 0046225X NLM ISO Abbreviation: Environ Entomol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Oxford University Press Original Publication: College Park, Md., Entomological Society of America. |
| مواضيع طبية MeSH: | Pyrethrins*/pharmacology , Insect Repellents*/pharmacology , Insect Repellents*/analysis , Aedes*/physiology, Female ; Animals ; Carbon Dioxide/pharmacology ; Cues ; Mosquito Vectors ; Mosquito Control/methods ; Atmosphere |
| مستخلص: | Spatial repellents are emerging as a promising approach to reduce vector-disease burden; however, the evolution of genetically resistant mosquitoes decreases repellent efficacy. The development of flight chambers to investigate spatial repellent application techniques is vital for sustainable mosquito control. We present an air-dilution chamber as a novel bioassay to study mosquito flight behavior responses to chemical gradients of the volatile, pyrethroid transfluthrin (TF). Air dilution was used to simulate a larger environment of stable concentration gradients verified with carbon dioxide (CO2) which was homogenously delivered and measured across the chamber to achieve a 5× inlet/outlet [CO2] ratio with 0.17 m/s outlet velocity. Female Aedes (Ae.) aegypti (Diptera: Culicidae, Linnaeus, 1762) were exposed to volatilized TF paired with heat, CO2, and Biogents-Sweetscent host-cues. Tandem solvent extraction-gas chromatography-mass spectrometry (SE-GC-MS) was used to quantify air samples taken during TF emanations with a limit of detection (LOD) and quantification (LOQ) of 2 ± 1 and 5 ± 2 parts-per-trillion (ppt) TF, respectively. Homogenous air diluted emanation of the spatial repellent TF was at least twice that of the 5× CO2 gradient with the same air flow in the chamber. The airborne TF concentrations the mosquitoes were exposed to range from 1 to 170 ppt. Video recordings of mosquito behavior during host-cues exposure revealed increased inlet activity, while exposure to TF protected host resulted in decreased inlet activity over time with inlet-outlet mosquito positional variation. This novel flight chamber design can simulate 'long'-range exposure with simultaneous quantitation of airborne spatial repellent to understand dose-dependent effects on mosquito behavior. (© The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| فهرسة مساهمة: | Keywords: Aedes aegypti; flight chamber; olfactometer; pyrethroid; transfluthrin |
| المشرفين على المادة: | 0 (Pyrethrins) 142M471B3J (Carbon Dioxide) 0 (Insect Repellents) QWL3SKA6EG (transfluthrin) |
| تواريخ الأحداث: | Date Created: 20230216 Date Completed: 20230420 Latest Revision: 20230501 |
| رمز التحديث: | 20230501 |
| DOI: | 10.1093/ee/nvad007 |
| PMID: | 36794837 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1938-2936 |
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| DOI: | 10.1093/ee/nvad007 |