دورية أكاديمية
Identification and characterization of two long-type peptidoglycan recognition proteins, PGRP-L1 and PGRP-L2, in the orange-spotted grouper, Epinephelus coioides.
| العنوان: | Identification and characterization of two long-type peptidoglycan recognition proteins, PGRP-L1 and PGRP-L2, in the orange-spotted grouper, Epinephelus coioides. |
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| المؤلفون: | Hou J; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, Guangdong, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of fishery, Guangdong Ocean University, Zhanjiang, 524025, Guangdong, China., Hao W; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China., Chang Li M; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China., Gan Z; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, Guangdong, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of fishery, Guangdong Ocean University, Zhanjiang, 524025, Guangdong, China., Chen SN; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China., Lu YS; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, Guangdong, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of fishery, Guangdong Ocean University, Zhanjiang, 524025, Guangdong, China. Electronic address: fishdis@163.com., Xia LQ; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, Guangdong, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, College of fishery, Guangdong Ocean University, Zhanjiang, 524025, Guangdong, China. Electronic address: xialq@gdou.edu.cn. |
| المصدر: | Fish & shellfish immunology [Fish Shellfish Immunol] 2023 Mar; Vol. 134, pp. 108580. Date of Electronic Publication: 2023 Feb 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; New York : Academic Press, c1991- |
| مواضيع طبية MeSH: | Bass*, Animals ; Phylogeny ; Carrier Proteins/genetics ; Amino Acid Sequence ; Peptidoglycan/metabolism |
| مستخلص: | Peptidoglycan recognition proteins (PGRPs) play an important role in innate immunity by recognizing components of pathogenic bacteria (such as peptidoglycan, PGN) and are evolutionarily conserved pattern recognition receptors (PRRs) in both invertebrates and vertebrates. In the present study, two long-type PGRPs (designed as Eco-PGRP-L1 and Eco-PGRP-L2) were identified in orange-spotted grouper (Epinephelus coioides), which is a major economic species cultured in Asia. The predicted protein sequences of both Eco-PGRP-L1 and Eco-PGRP-L2 contain a typical PGRP domain. Eco-PGRP-L1 and Eco-PGRP-L2 exhibited organ/tissue-specific expression patterns. An abundant expression of Eco-PGRP-L1 was observed in pyloric caecum, stomach and gill, whereas a highest expression level of Eco-PGRP-L2 was found in head kidney, spleen, skin and heart. In addition, Eco-PGRP-L1 is distributed in the cytoplasm and nucleus, while Eco-PGRP-L2 is mainly localized in cytoplasm. Both Eco-PGRP-L1 and Eco-PGRP-L2 were induced following the stimulation of PGN and have PGN binding activity. In addition, functional analysis revealed that Eco-PGRP-L1 and Eco-PGRP-L2 possess antibacterial activity against Edwardsiella tarda. These results may contribute to understand the innate immune system of orange-spotted grouper. Competing Interests: Declaration of competing interest The authors report no declarations of interest. (Copyright © 2023. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Antibacterial activity; Orange-spotted grouper; PGRP; Peptidoglycan recognition proteins |
| المشرفين على المادة: | 0 (peptidoglycan recognition protein) 0 (Carrier Proteins) 0 (Peptidoglycan) |
| تواريخ الأحداث: | Date Created: 20230216 Date Completed: 20230314 Latest Revision: 20230314 |
| رمز التحديث: | 20230314 |
| DOI: | 10.1016/j.fsi.2023.108580 |
| PMID: | 36796596 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9947 |
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| DOI: | 10.1016/j.fsi.2023.108580 |