Ex vivo RSA and Pfkelch13 targeted-amplicon deep sequencing reveal parasites susceptibility to artemisinin in Senegal, 2017.
| العنوان: | Ex vivo RSA and Pfkelch13 targeted-amplicon deep sequencing reveal parasites susceptibility to artemisinin in Senegal, 2017. |
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| المؤلفون: | Yade MS; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Dièye B; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Coppée R; Université Paris Cité and Sorbone Paris Nord, Inserm, IAME., Mbaye A; Centre for Research and Training in Infectiology of Guinea (CRTIG)., Diallo MA; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Diongue K; Cheikh Anta Diop University of Dakar., Bailly J; University of Paris Cité, IRD, MERIT., Mama A; Université de Paris, Institut Cochin, Inserm U1016., Fall A; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Thiaw AB; Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences., Ndiaye IM; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Ndiaye T; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Gaye A; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Tine A; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Diédhiou Y; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Mbaye AM; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Doderer-Lang C; Université de Strasbourg, UR7292 Dynamics of Host-Pathogen Interactions., Garba MN; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar., Bei AK; Yale School of Public Health., Ménard D; Université de Strasbourg, UR7292 Dynamics of Host-Pathogen Interactions., Ndiaye D; Centre International de Recherche et de Formation en Génomique Appliquée, et de Surveillance Sanitaire (CIGASS), Cheikh Anta Diop University of Dakar. |
| المصدر: | Research square [Res Sq] 2023 Feb 06. Date of Electronic Publication: 2023 Feb 06. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101768035 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: Res Sq Subsets: PubMed not MEDLINE |
| مستخلص: | Introduction: Malaria control is highly dependent on the effectiveness of artemisinin-based combination therapies (ACTs), the current frontline malaria curative treatments. Unfortunately, the emergence and spread of parasites resistant to artemisinin (ART) derivatives in Southeast Asia and South America, and more recently in Rwanda and Uganda (East Africa), compromise their long-term use in Sub-Saharan Africa where most malaria deaths occur. Methods: Here, we evaluated ex vivo susceptibility to dihydroartemisinin (DHA) from 38 P. falciparum isolates collected in 2017 in Thiès (Senegal) expressed with the Ring-stage Survival Assay (RSA). We explored major and minor variants in the full Pfkelch13 gene, the main determinant of ART resistance using a targeted-amplicon deep sequencing (TADS) approach. Results: All samples tested in the ex vivo RSA were found to be susceptible to DHA. Both non-synonymous mutations K189T and K248R were observed each in one isolate, as major (99%) or minor (5%) variants, respectively. Conclusion: Altogether, investigations combining ex vivo RSA and TADS are a useful approach for monitoring ART resistance in Africa. |
| التعليقات: | Update in: Malar J. 2023 May 26;22(1):167. doi: 10.1186/s12936-023-04588-1. (PMID: 37237307) |
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| معلومات مُعتمدة: | U19 AI089696 United States AI NIAID NIH HHS |
| تواريخ الأحداث: | Date Created: 20230217 Latest Revision: 20240626 |
| رمز التحديث: | 20240626 |
| مُعرف محوري في PubMed: | PMC9934778 |
| DOI: | 10.21203/rs.3.rs-2538775/v1 |
| PMID: | 36798264 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.21203/rs.3.rs-2538775/v1 |
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