تقرير
Feasibility of moxifloxacin and proflavine dual fluorescence imaging for detecting gastrointestinal neoplastic lesions: A prospective study.
العنوان: | Feasibility of moxifloxacin and proflavine dual fluorescence imaging for detecting gastrointestinal neoplastic lesions: A prospective study. |
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المؤلفون: | Nam K; Department of Gastroenterology, Dankook University Hospital, Dankook University College of Medicine, Cheonan, South Korea.; Digestive Diseases Research Center, University of Ulsan College of Medicine, Seoul, South Korea., Park N; Division of Interdisciplinary Bioscience and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, South Korea., Lee S; Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea., Jeon S; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, South Korea., Lee J; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, South Korea., Hong SM; Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Hwang SW; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Park SH; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Yang DH; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Ye BD; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Byeon JS; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Yang SK; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Lee JH; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Kim DH; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea., Kim KH; Division of Interdisciplinary Bioscience and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, South Korea.; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, South Korea., Myung SJ; Digestive Diseases Research Center, University of Ulsan College of Medicine, Seoul, South Korea.; Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.; EDIS Biotech, Seoul, South Korea. |
المصدر: | Lasers in surgery and medicine [Lasers Surg Med] 2023 Apr; Vol. 55 (4), pp. 378-389. Date of Electronic Publication: 2023 Feb 17. |
نوع المنشور: | Case Reports; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8007168 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-9101 (Electronic) Linking ISSN: 01968092 NLM ISO Abbreviation: Lasers Surg Med Subsets: MEDLINE |
أسماء مطبوعة: | Publication: New York Ny : Wiley-Liss Original Publication: New York : Alan Liss |
مواضيع طبية MeSH: | Proflavine* , Adenoma*/pathology, Humans ; Moxifloxacin ; Prospective Studies ; Feasibility Studies ; Optical Imaging |
مستخلص: | Objectives: High-contrast and high-resolution imaging techniques would enable real-time sensitive detection of the gastrointestinal lesions. This study aimed to investigate the feasibility of novel dual fluorescence imaging using moxifloxacin and proflavine in the detection of neoplastic lesions of the human gastrointestinal tract. Methods: Patients with the colonic and gastric neoplastic lesions were prospectively enrolled. The lesions were biopsied with forceps or endoscopically resected. Dual fluorescence imaging was performed by using custom axially swept wide-field fluorescence microscopy after topical moxifloxacin and proflavine instillation. Imaging results were compared with both confocal imaging with cell labeling and conventional histological examination. Results: Ten colonic samples (one normal mucosa, nine adenomas) from eight patients and six gastric samples (one normal mucosa, five adenomas) from four patients were evaluated. Dual fluorescence imaging visualized detail cellular structures. Regular glandular structures with polarized cell arrangement were observed in normal mucosa. Goblet cells were preserved in normal colonic mucosa. Irregular glandular structures with scanty cytoplasm and dispersed elongated nuclei were observed in adenomas. Goblet cells were scarce or lost in the colonic lesions. Similarity analysis between moxifloxacin and proflavine imaging showed relatively high correlation values in adenoma compared with those in normal mucosa. Dual fluorescence imaging showed good detection accuracies of 82.3% and 86.0% in the colonic and the gastric lesions, respectively. Conclusions: High-contrast and high-resolution dual fluorescence imaging was feasible for obtaining detail histopathological information in the gastrointestinal neoplastic lesions. Further studies are needed to develop dual fluorescence imaging as an in vivo real-time visual diagnostic method. (© 2023 Wiley Periodicals LLC.) |
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فهرسة مساهمة: | Keywords: fluorescence imaging; gastrointestinal neoplasia; moxifloxacin; proflavine |
المشرفين على المادة: | CY3RNB3K4T (Proflavine) U188XYD42P (Moxifloxacin) |
تواريخ الأحداث: | Date Created: 20230221 Date Completed: 20230413 Latest Revision: 20230523 |
رمز التحديث: | 20231215 |
DOI: | 10.1002/lsm.23640 |
PMID: | 36802075 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1096-9101 |
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DOI: | 10.1002/lsm.23640 |