دورية أكاديمية
Action of crude ethanol extract of Hymenaea martiana leaf, gallic acid, and polypyrrole (PPy) against Aeromonas hydrophila.
| العنوان: | Action of crude ethanol extract of Hymenaea martiana leaf, gallic acid, and polypyrrole (PPy) against Aeromonas hydrophila. |
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| المؤلفون: | Meira TM; Universidade Federal do Vale do São Franscisco, Petrolina, Pernambuco, Brazil., da Costa MM; Universidade Federal do Vale do São Franscisco, Petrolina, Pernambuco, Brazil., de Simoni Gouveia JJ; Universidade Federal do Vale do São Franscisco, Petrolina, Pernambuco, Brazil., Soares RAN; Universidade Federal do Vale do São Franscisco, Petrolina, Pernambuco, Brazil., Tavares MRS; Instituto Federal do Sertão Pernambucano - Zona Rural, Petrolina, Pernambuco, Brazil., Fernandes AWC; Faculdade Estácio - Juazeiro, Juazeiro, Bahia, Brazil., Gouveia GV; Universidade Federal do Vale do São Franscisco, Petrolina, Pernambuco, Brazil. gisele.veneroni@univasf.edu.br. |
| المصدر: | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Jun; Vol. 54 (2), pp. 1191-1202. Date of Electronic Publication: 2023 Feb 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Switzerland, AG : Springer International Publishing Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia |
| مواضيع طبية MeSH: | Hymenaea* , Anti-Infective Agents*/pharmacology, Animals ; Polymers ; Gallic Acid/pharmacology ; Ethanol/pharmacology ; Aeromonas hydrophila ; Pyrroles/pharmacology ; Molecular Docking Simulation ; Anti-Bacterial Agents/pharmacology ; Microbial Sensitivity Tests ; Plant Extracts/pharmacology ; Plant Leaves |
| مستخلص: | Pisciculture represents one of the industries with the fastest growth rates worldwide. However, it presents obstacles to its development, such as bacteriosis, which is conventionally treated with antibiotics. The indiscriminate and inappropriate use of antibiotics can lead to bacterial resistance, thus alternatives to the use of antibiotics have been researched. The study aimed to analyze the potential of crude ethanol extract (CEE) from Hymenaea martiana leaf, gallic acid (GA), and polypyrrole (PPy) against Aeromonas hydrophila. Tests were performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the compounds individually and in synergy (checkerboard) against A. hydrophila and in silico tests between the compounds evaluated. The CEE of H. martiana leaf and PPy were effective against A. hydrophila with MBC results of 3125 μg/mL for the CEE of H. martiana and 125 μg/mL for PPy. Evaluating the GA, a MIC and MBC of 125 μg/mL was obtained. In the interaction tests (checkerboard, using PPy/CEE and PPy/GA), there was a significant reduction in individual introductions. Thus, for the PPy/CEE tests, we had a reduction of MIC/MBC to 1.95 and 781.25 μg/mL, and for the synergy tests between PPy/GA to 7.8125 and 31.125 μg/mL, respectively. The synergy tests are encouraging, and it is possible to verify a decrease of up to 98% in the introduction of PPy, 75% in CEE for H. martiana and 75.1% for GA, when compared to their individual tests. The tests with GA are encouraging due to GA's effectiveness as an antimicrobial agent and high synergy with polypyrrole, both in vitro results and molecular docking experiments showed the actions at the same activation site in A. hydrophila. In vivo tests evaluating isolated components of CEE from H. martiana in synergy with PPy should be performed, to verify the quality of the interactions and the improvement of the immune responses of the animals. It was evidenced that gallic acid, a substance isolated from the extract, tends to have more promising results. This is relevant since the industry has been developing these compounds for different uses, thus providing easier access to the product. Thus, the present study indicates an efficient alternative in the use of bioactive compounds as substitutes for conventional antimicrobials. (© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.) |
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| فهرسة مساهمة: | Keywords: Conductive polymers; Phytotherapeutic; Sustainable production; Synergy |
| المشرفين على المادة: | 30604-81-0 (polypyrrole) 0 (Polymers) 632XD903SP (Gallic Acid) 3K9958V90M (Ethanol) 0 (Pyrroles) 0 (Anti-Bacterial Agents) 0 (Anti-Infective Agents) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20230222 Date Completed: 20230605 Latest Revision: 20240219 |
| رمز التحديث: | 20240219 |
| مُعرف محوري في PubMed: | PMC10235323 |
| DOI: | 10.1007/s42770-023-00922-6 |
| PMID: | 36807089 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1678-4405 |
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| DOI: | 10.1007/s42770-023-00922-6 |