دورية أكاديمية
أعرض في EDS

Micafungin effect on Pseudomonas aeruginosa metabolome, virulence and biofilm: potential quorum sensing inhibitor.

التفاصيل البيبلوغرافية
العنوان: Micafungin effect on Pseudomonas aeruginosa metabolome, virulence and biofilm: potential quorum sensing inhibitor.
المؤلفون: Hijazi DM; Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, 11942, Jordan., Dahabiyeh LA; Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, 11942, Jordan. l.dahabiyeh@ju.edu.jo., Abdelrazig S; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, 1996, 11115, Khartoum, Sudan.; Centre for Analytical Bioscience, Advanced Materials and Healthcare Technologies Division, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK., Alqudah DA; Cell Therapy Center, The University of Jordan, Amman, 11942, Jordan., Al-Bakri AG; Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, The University of Jordan, Amman, 11942, Jordan. agbakri@ju.edu.jo.
المصدر: AMB Express [AMB Express] 2023 Feb 20; Vol. 13 (1), pp. 20. Date of Electronic Publication: 2023 Feb 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101561785 Publication Model: Electronic Cited Medium: Print ISSN: 2191-0855 (Print) Linking ISSN: 21910855 NLM ISO Abbreviation: AMB Express Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Heidelberg : Springer-Verlag, c2011-
مستخلص: The prevalence of antibiotic resistance in Pseudomonas aeruginosa places a heavy burden on the health care sectors urging the need to find alternative, non-antibiotic strategies. The interference with the P. aeruginosa quorum sensing (QS) system represents a promising alternative strategy to attenuate the bacterial virulency and its ability to form biofilms. Micafungin has been reported to impede the pseudomonal biofilm formation. However, the influences of micafungin on the biochemical composition and metabolites levels of P. aeruginosa have not been explored. In this study, the effect of micafungin (100 µg/mL) on the virulence factors, QS signal molecules and the metabolome of P. aeruginosa was studied using exofactor assay and mass spectrometry-based metabolomics approaches. Furthermore, confocal laser scanning microscopy (CLSM) using the fluorescent dyes ConA-FITC and SYPRO® Ruby was used to visualize micafungin disturbing effects on the pseudomonal glycocalyx and protein biofilm-constituents, respectively. Our findings showed that micafungin significantly decreased the production of various QS-controlled virulence factors (pyocyanin, pyoverdine, pyochelin and rhamnolipid), along with a dysregulation in the level of various metabolites involved in QS system, lysine degradation, tryptophan biosynthesis, TCA cycle, and biotin metabolism. In addition, the CLSM examination showed an altered matrix distribution. The presented findings highlight the promising role of micafungin as a potential quorum sensing inhibitor (QSI) and anti-biofilm agent to attenuate P. aeruginosa pathogenicity. In addition, they point to the promising role of metabolomics study in investigating the altered biochemical pathways in P. aeruginosa.
(© 2023. The Author(s).)
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فهرسة مساهمة: Keywords: Biofilm; Metabolomics; Micafungin; Pseudomonas aeruginosa; Quorum sensing; Virulence factors
تواريخ الأحداث: Date Created: 20230222 Latest Revision: 20230225
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9941417
DOI: 10.1186/s13568-023-01523-0
PMID: 36807839
قاعدة البيانات: MEDLINE
الوصف
تدمد:2191-0855
DOI:10.1186/s13568-023-01523-0