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Vitamin D3 improved erectile function recovery by regulating autophagy and apoptosis in a rat model of cavernous nerve injury.

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العنوان: Vitamin D3 improved erectile function recovery by regulating autophagy and apoptosis in a rat model of cavernous nerve injury.
المؤلفون: Zhu S; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China., Xiong Y; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China., Yu B; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, Ningbo Medical Center, Lihuili Hospital, Ningbo, China., Wang H; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China., Zhang F; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China., Wu C; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China., Qin F; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China., Yuan J; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China. jiuhongyuan2107@163.com.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China. jiuhongyuan2107@163.com.
المصدر: International journal of impotence research [Int J Impot Res] 2024 Jun; Vol. 36 (4), pp. 430-436. Date of Electronic Publication: 2023 Feb 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 9007383 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5489 (Electronic) Linking ISSN: 09559930 NLM ISO Abbreviation: Int J Impot Res Subsets: MEDLINE
أسماء مطبوعة: Publication: Basingstoke : Nature Publishing Group
Original Publication: London, UK : Smith-Gordon, [1989-
مواضيع طبية MeSH: Autophagy*/drug effects , Apoptosis*/drug effects , Rats, Sprague-Dawley* , Cholecalciferol*/pharmacology , Erectile Dysfunction*/drug therapy , Erectile Dysfunction*/etiology , Penis*/innervation , Penis*/drug effects , Penile Erection*/drug effects, Animals ; Male ; Rats ; Disease Models, Animal ; Recovery of Function/drug effects ; Peripheral Nerve Injuries/drug therapy ; Fibrosis
مستخلص: Vitamin D 3 is an important element in improving erectile function. However, the mechanisms of vitamin D 3 remain unknown. Thus, we explored the effect of vitamin D 3 on erectile function recovery after nerve injury in a rat model and investigated its possible molecular mechanisms. Eighteen male Sprague-Dawley rats were used in this study. The rats were randomly divided into three groups: the control, bilateral cavernous nerve crush (BCNC), and BCNC + vitamin D 3 groups. BCNC model was established in rats by surgery. The intracavernosal pressure and the ratio of intracavernosal pressure to mean arterial pressure were utilized to evaluate erectile function. Masson trichrome staining, immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling and western blot analysis were performed on penile tissues to elucidate the molecular mechanism. The results indicated that vitamin D 3 alleviated hypoxia and suppressed the fibrosis signalling pathway by upregulating the expression of eNOS (p = 0.001), nNOS (p = 0.018) and α-SMA (p = 0.025) and downregulating the expression of HIF-1α (p = 0.048) and TGF-β1 (p = 0.034) in BCNC rats. Vitamin D 3 promoted erectile function restoration by enhancing the autophagy process through decreases in the p-mTOR/mTOR ratio (p = 0.02) and p62 (p = 0.001) expression and increases in Beclin1 expression (p = 0.001) and the LC3B/LC3A ratio (p = 0.041). Vitamin D 3 application improved erectile function rehabilitation by suppressing the apoptotic process through decreases in the expression of Bax (p = 0.002) and caspase-3 (p = 0.046) and an increase in the expression of Bcl2 (p = 0.004). Therefore, We concluded that vitamin D 3 improved the erectile function recovery in BCNC rats by alleviating hypoxia and fibrosis, enhancing autophagy and inhibiting apoptosis in the corpus cavernosum.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
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المشرفين على المادة: 1C6V77QF41 (Cholecalciferol)
تواريخ الأحداث: Date Created: 20230222 Date Completed: 20240527 Latest Revision: 20240527
رمز التحديث: 20240528
DOI: 10.1038/s41443-023-00679-4
PMID: 36813836
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-5489
DOI:10.1038/s41443-023-00679-4