دورية أكاديمية
Local irradiation of patient-derived tumors in immunodeficient mice.
| العنوان: | Local irradiation of patient-derived tumors in immunodeficient mice. |
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| المؤلفون: | van der Zalm AP; Amsterdam UMC Location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory of Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands; Oncode Institute, Amsterdam, the Netherlands; Cancer Center Amsterdam, Cancer Biology, De Boelelaan 1118, 1081HV Amsterdam, the Netherlands., Bootsma S; Amsterdam UMC Location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory of Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands; Oncode Institute, Amsterdam, the Netherlands; Cancer Center Amsterdam, Cancer Biology, De Boelelaan 1118, 1081HV Amsterdam, the Netherlands., Rodermond HM; Amsterdam UMC Location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory of Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands; Cancer Center Amsterdam, Cancer Biology, De Boelelaan 1118, 1081HV Amsterdam, the Netherlands; Amsterdam UMC Location University of Amsterdam, Department of Radiation Oncology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands., Oei AL; Amsterdam UMC Location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory of Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands; Cancer Center Amsterdam, Cancer Biology, De Boelelaan 1118, 1081HV Amsterdam, the Netherlands; Amsterdam UMC Location University of Amsterdam, Department of Radiation Oncology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands. Electronic address: a.l.oei@amsterdamumc.nl., Bijlsma MF; Amsterdam UMC Location University of Amsterdam, Center for Experimental and Molecular Medicine, Laboratory of Experimental Oncology and Radiobiology, Meibergdreef 9, 1105AZ Amsterdam, the Netherlands; Oncode Institute, Amsterdam, the Netherlands; Cancer Center Amsterdam, Cancer Biology, De Boelelaan 1118, 1081HV Amsterdam, the Netherlands. |
| المصدر: | STAR protocols [STAR Protoc] 2023 Mar 17; Vol. 4 (1), pp. 102098. Date of Electronic Publication: 2023 Feb 07. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Neoplasms*/radiotherapy, Humans ; Mice ; Animals ; Mice, SCID |
| مستخلص: | Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkd scid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022). 1 . Competing Interests: Declaration of interests M.F.B. has received research funding from Celgene, Frame Therapeutics, and Lead Pharma and has acted as a consultant to Servier. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cancer; Cell Culture; Health Sciences; Microscopy; Model Organisms |
| تواريخ الأحداث: | Date Created: 20230224 Date Completed: 20231023 Latest Revision: 20231026 |
| رمز التحديث: | 20231027 |
| مُعرف محوري في PubMed: | PMC9929630 |
| DOI: | 10.1016/j.xpro.2023.102098 |
| PMID: | 36825807 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2023.102098 |