دورية أكاديمية
أعرض في EDS

Carboxylated ε-Poly-l-lysine Improves Post-Thaw Quality, Mitochondrial Functions and Antioxidant Defense of Goat Cryopreserved Sperm.

التفاصيل البيبلوغرافية
العنوان: Carboxylated ε-Poly-l-lysine Improves Post-Thaw Quality, Mitochondrial Functions and Antioxidant Defense of Goat Cryopreserved Sperm.
المؤلفون: Zhang W; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Cui H; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Ding K; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Zhou K; Shandong Provincial Animal Husbandry General Station, Jinan 250022, China., Li Y; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Hoque SAM; Department of Animal Breeding and Genetics, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh., Min L; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Zhu Z; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
المصدر: Biology [Biology (Basel)] 2023 Feb 01; Vol. 12 (2). Date of Electronic Publication: 2023 Feb 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101587988 Publication Model: Electronic Cited Medium: Print ISSN: 2079-7737 (Print) Linking ISSN: 20797737 NLM ISO Abbreviation: Biology (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, 2012-
مستخلص: Carboxylated ε-poly-l-lysine (CPLL), a novel cryoprotectant, can protect the sperm membranes by inhibiting ice crystal formation during the cryopreservation process. The present study was conducted to investigate the consequence of CPLL supplementation on the post-thaw quality of cryopreserved goat sperm. For this, different doses (0, 0.5%, 1%, 1.5%, and 2%; v / v ) of CPLL were added to the cryopreservation medium, and the motility, membrane and acrosome integrity, mitochondrial membrane potential (MMP), ATP level, ROS production, anti-oxidant defense system, malondialdehyde (MDA) level, and apoptosis in post-thaw sperm were evaluated. It was observed that the addition of 1% CPLL significantly ( p < 0.05) increased the total motility, membrane integrity, acrosome integrity, and catalase (CAT) activity of post-thaw sperm compared to those of control and other CPLL doses. The ATP content was observed significantly ( p < 0.05) higher in 0.5% and 1% CPLL, however, the SOD activity and progressive motility were significantly ( p < 0.05) increased by adding CPLL at 1% and 1.5% level. Moreover, the addition of CPLL at 1% dose not only showed a lower percentage of apoptosis, but also significantly ( p < 0.05) increased the MMP while reducing ROS production and MDA levels compared to those of other CPLL doses and/or control. Therefore, it is clear that the supplementation of 1% CPLL can remarkably improve the post-thaw goat sperm motility, membrane and acrosome integrity, antioxidant abundance, mitochondrial potentials, and ATP supply by protecting the sperm from cryodamage and undergoing apoptosis. These findings will provide novel insights into sperm cryobiology.
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معلومات مُعتمدة: 1121010 Start-up Fund for High-level Talents of Qingdao Agricultural University for Z Zhu; YDZX2021113 the Shandong Province Central Guided Local Science and Technology Development Project for Z Zhu; SDAIT-10-08 Technology System of Modern Agricultural Industry in Shandong Province
فهرسة مساهمة: Keywords: CPLL; cryopreservation; goat sperm; quality
تواريخ الأحداث: Date Created: 20230225 Latest Revision: 20230228
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9953348
DOI: 10.3390/biology12020231
PMID: 36829509
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-7737
DOI:10.3390/biology12020231