دورية أكاديمية
Carboxylated ε-Poly-l-lysine Improves Post-Thaw Quality, Mitochondrial Functions and Antioxidant Defense of Goat Cryopreserved Sperm.
العنوان: | Carboxylated ε-Poly-l-lysine Improves Post-Thaw Quality, Mitochondrial Functions and Antioxidant Defense of Goat Cryopreserved Sperm. |
---|---|
المؤلفون: | Zhang W; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Cui H; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Ding K; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Zhou K; Shandong Provincial Animal Husbandry General Station, Jinan 250022, China., Li Y; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Hoque SAM; Department of Animal Breeding and Genetics, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh., Min L; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China., Zhu Z; College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China. |
المصدر: | Biology [Biology (Basel)] 2023 Feb 01; Vol. 12 (2). Date of Electronic Publication: 2023 Feb 01. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101587988 Publication Model: Electronic Cited Medium: Print ISSN: 2079-7737 (Print) Linking ISSN: 20797737 NLM ISO Abbreviation: Biology (Basel) Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, 2012- |
مستخلص: | Carboxylated ε-poly-l-lysine (CPLL), a novel cryoprotectant, can protect the sperm membranes by inhibiting ice crystal formation during the cryopreservation process. The present study was conducted to investigate the consequence of CPLL supplementation on the post-thaw quality of cryopreserved goat sperm. For this, different doses (0, 0.5%, 1%, 1.5%, and 2%; v / v ) of CPLL were added to the cryopreservation medium, and the motility, membrane and acrosome integrity, mitochondrial membrane potential (MMP), ATP level, ROS production, anti-oxidant defense system, malondialdehyde (MDA) level, and apoptosis in post-thaw sperm were evaluated. It was observed that the addition of 1% CPLL significantly ( p < 0.05) increased the total motility, membrane integrity, acrosome integrity, and catalase (CAT) activity of post-thaw sperm compared to those of control and other CPLL doses. The ATP content was observed significantly ( p < 0.05) higher in 0.5% and 1% CPLL, however, the SOD activity and progressive motility were significantly ( p < 0.05) increased by adding CPLL at 1% and 1.5% level. Moreover, the addition of CPLL at 1% dose not only showed a lower percentage of apoptosis, but also significantly ( p < 0.05) increased the MMP while reducing ROS production and MDA levels compared to those of other CPLL doses and/or control. Therefore, it is clear that the supplementation of 1% CPLL can remarkably improve the post-thaw goat sperm motility, membrane and acrosome integrity, antioxidant abundance, mitochondrial potentials, and ATP supply by protecting the sperm from cryodamage and undergoing apoptosis. These findings will provide novel insights into sperm cryobiology. |
References: | Theriogenology. 2020 Mar 1;144:8-15. (PMID: 31884337) Cryobiology. 2019 Jun;88:70-74. (PMID: 30922739) Cells. 2021 Jun 08;10(6):. (PMID: 34201225) J Anim Sci. 2022 Apr 1;100(4):. (PMID: 35247050) Exp Gerontol. 2001 Feb;36(2):209-20. (PMID: 11226737) Adv Sci (Weinh). 2019 Jan 27;6(6):1802045. (PMID: 30937270) Mol Med. 2022 Aug 3;28(1):88. (PMID: 35922749) Biomolecules. 2019 Mar 19;9(3):. (PMID: 30893949) BMC Cell Biol. 2013 Jul 09;14:32. (PMID: 23834359) Cryobiology. 2022 Jun;106:13-23. (PMID: 35550791) Andrology. 2022 Sep;10(6):1123-1133. (PMID: 35712876) Cryo Letters. 2016 Mar-Apr;37(2):123-8. (PMID: 27224524) Biomacromolecules. 2016 May 9;17(5):1882-93. (PMID: 27077533) Biotechnol Biofuels Bioprod. 2022 Jun 16;15(1):65. (PMID: 35710433) Animals (Basel). 2020 Sep 01;10(9):. (PMID: 32883027) PLoS One. 2017 Apr 27;12(4):e0176711. (PMID: 28448636) Free Radic Biol Med. 2019 Sep;141:159-171. (PMID: 31212063) Stem Cells. 2006 Nov;24(11):2549-56. (PMID: 16840553) J Exp Med. 1995 Aug 1;182(2):367-77. (PMID: 7629499) Biomaterials. 2009 Sep;30(27):4842-9. (PMID: 19515417) Cryobiology. 2017 Jun;76:140-145. (PMID: 28161239) Cell Transplant. 2021 Jan-Dec;30:963689721999617. (PMID: 33757335) Biopreserv Biobank. 2019 Dec;17(6):603-612. (PMID: 31429586) Reprod Biomed Online. 2018 Sep;37(3):327-339. (PMID: 30143329) Clin Exp Reprod Med. 2022 Jun;49(2):117-126. (PMID: 35698774) Sci Rep. 2021 Nov 19;11(1):22563. (PMID: 34799600) Theriogenology. 2007 Sep 15;68(5):813-9. (PMID: 17658594) Oxid Med Cell Longev. 2019 Sep 4;2019:5921503. (PMID: 31565152) Reprod Domest Anim. 2015 Jul;50 Suppl 2:71-9. (PMID: 26174922) Animals (Basel). 2022 Jul 04;12(13):. (PMID: 35804625) Methods Mol Biol. 2021;2180:365-377. (PMID: 32797421) Reprod Biol Endocrinol. 2020 Mar 7;18(1):17. (PMID: 32145746) Biotechnol Bioeng. 2009 Nov 1;104(4):719-28. (PMID: 19593758) Int J Biol Macromol. 2021 Oct 31;189:292-305. (PMID: 34419548) PLoS One. 2013 Dec 23;8(12):e83613. (PMID: 24376724) Adv Exp Med Biol. 2008;636:154-71. (PMID: 19856167) Anim Reprod Sci. 2015 Jun;157:56-62. (PMID: 25863987) Hum Reprod. 1997 Jan;12(1):112-8. (PMID: 9043914) Rev Reprod. 1997 Jan;2(1):48-54. (PMID: 9414465) Reprod Domest Anim. 2019 Apr;54(4):655-665. (PMID: 30681204) Animals (Basel). 2022 Sep 30;12(19):. (PMID: 36230367) |
معلومات مُعتمدة: | 1121010 Start-up Fund for High-level Talents of Qingdao Agricultural University for Z Zhu; YDZX2021113 the Shandong Province Central Guided Local Science and Technology Development Project for Z Zhu; SDAIT-10-08 Technology System of Modern Agricultural Industry in Shandong Province |
فهرسة مساهمة: | Keywords: CPLL; cryopreservation; goat sperm; quality |
تواريخ الأحداث: | Date Created: 20230225 Latest Revision: 20230228 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9953348 |
DOI: | 10.3390/biology12020231 |
PMID: | 36829509 |
قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!