دورية أكاديمية
Isolation and Characterization of the First Zobellviridae Family Bacteriophage Infecting Klebsiella pneumoniae .
العنوان: | Isolation and Characterization of the First Zobellviridae Family Bacteriophage Infecting Klebsiella pneumoniae . |
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المؤلفون: | Gorodnichev RB; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Kornienko MA; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Malakhova MV; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Bespiatykh DA; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Manuvera VA; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Selezneva OV; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Veselovsky VA; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Bagrov DV; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.; Department of Bioengineering, Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia., Zaychikova MV; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Osnach VA; Saint Petersburg Pasteur Institute, Federal Service on Consumer Rights Protection and Human Well-Being Surveillance, 197101 St. Petersburg, Russia., Shabalina AV; Saint Petersburg Pasteur Institute, Federal Service on Consumer Rights Protection and Human Well-Being Surveillance, 197101 St. Petersburg, Russia., Goloshchapov OV; R.M. Gorbacheva Memorial Institute of Oncology, Hematology and Transplantation, Pavlov First Saint Petersburg State Medical University, 197022 St. Petersburg, Russia., Bespyatykh JA; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia., Dolgova AS; Saint Petersburg Pasteur Institute, Federal Service on Consumer Rights Protection and Human Well-Being Surveillance, 197101 St. Petersburg, Russia., Shitikov EA; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia. |
المصدر: | International journal of molecular sciences [Int J Mol Sci] 2023 Feb 17; Vol. 24 (4). Date of Electronic Publication: 2023 Feb 17. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
مواضيع طبية MeSH: | Bacteriophages*/genetics , Podoviridae*/genetics, Klebsiella pneumoniae/genetics ; Phylogeny ; Genome, Viral ; Recombinant Proteins/genetics |
مستخلص: | In order to address the upcoming crisis in the treatment of Klebsiella pneumoniae infections, caused by an increasing proportion of resistant isolates, new approaches to antimicrobial therapy must be developed. One approach would be to use (bacterio)phages and/or phage derivatives for therapy. In this study, we present a description of the first K. pneumoniae phage from the Zobellviridae family. The vB_KpnP_Klyazma podovirus, which forms translucent halos around the plaques, was isolated from river water. The phage genome is composed of 82 open reading frames, which are divided into two clusters located on opposite strands. Phylogenetic analysis revealed that the phage belongs to the Zobellviridae family, although its identity with the closest member of this family was not higher than 5%. The bacteriophage demonstrated lytic activity against all ( n = 11) K. pneumoniae strains with the KL20 capsule type, but only the host strain was lysed effectively. The receptor-binding protein of the phage was identified as a polysaccharide depolymerase with a pectate lyase domain. The recombinant depolymerase protein showed concentration-dependent activity against all strains with the KL20 capsule type. The ability of a recombinant depolymerase to cleave bacterial capsular polysaccharides regardless of a phage's ability to successfully infect a particular strain holds promise for the possibility of using depolymerases in antimicrobial therapy, even though they only make bacteria sensitive to environmental factors, rather than killing them directly. |
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معلومات مُعتمدة: | Code: Bacteriophage-2 State Assignment on the Development of a complex therapy scheme for drug-resistant pathogens of infectious diseases using bacteriophages or their derivatives in combination with antibacterial drugs |
فهرسة مساهمة: | Keywords: Klebsiella pneumoniae; Zobellviridae; bacteriophage; capsular type; capsule depolymerase; multidrug resistance |
المشرفين على المادة: | 0 (Recombinant Proteins) |
تواريخ الأحداث: | Date Created: 20230225 Date Completed: 20230228 Latest Revision: 20230228 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9960094 |
DOI: | 10.3390/ijms24044038 |
PMID: | 36835449 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1422-0067 |
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DOI: | 10.3390/ijms24044038 |