دورية أكاديمية
Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi.
العنوان: | Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi. |
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المؤلفون: | da Silva FMR; Laboratory of Natural Products and Mass Spectrometry (LaPNEM), Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil., Paggi GM; Laboratory of Ecology and Evolutionary Biology (LEBio), Institute of Biosciences, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil., Brust FR; Biofilms and Diversity Laboratory, Faculty of Pharmacy and Biotechnology Center, Federal University of Rio Grande do Sul, Porto Alegre 91501-970, Brazil., Macedo AJ; Biofilms and Diversity Laboratory, Faculty of Pharmacy and Biotechnology Center, Federal University of Rio Grande do Sul, Porto Alegre 91501-970, Brazil., Silva DB; Laboratory of Natural Products and Mass Spectrometry (LaPNEM), Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil. |
المصدر: | Metabolites [Metabolites] 2023 Feb 05; Vol. 13 (2). Date of Electronic Publication: 2023 Feb 05. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101578790 Publication Model: Electronic Cited Medium: Print ISSN: 2218-1989 (Print) Linking ISSN: 22181989 NLM ISO Abbreviation: Metabolites Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel : MDPI |
مستخلص: | Metabolomics strategies are important tools to get holistic chemical information from a system, but they are scarcely applied to endophytic fungi to understand their chemical profiles of biosynthesized metabolites. Here Penicillium sp. was cultured using One Strain Many Compounds (OSMAC) conditions as a model system to demonstrate how this strategy can help in understanding metabolic profiles and determining bioactive metabolites with the application of metabolomics and statistical analyses, as well as molecular networking. Penicillium sp. was fermented in different culture media and the crude extracts from mycelial biomass (CEm) and broth (CEb) were obtained, evaluated against bacterial strains ( Staphylococcus aureus and Pseudomonas aeruginosa ), and the metabolomic profiles by LC-DAD-MS were obtained and chemometrics statistical analyses were applied. The CEm and CEb extracts presented different chemical profiles and antibacterial activities; the highest activities observed were against S. aureus from CEm (MIC = 16, 64, and 128 µg/mL). The antibacterial properties from the extracts were impacted for culture media from which the strain was fermented. From the Volcano plot analysis, it was possible to determine statistically the most relevant features for the antibacterial activity, which were also confirmed from biplots of PCA as strong features for the bioactive extracts. These compounds included 75 (13-oxoverruculogen isomer), 78 (austalide P acid), 87 (austalide L or W), 88 (helvamide), 92 (viridicatumtoxin A), 96 (austalide P), 101 (dihydroaustalide K), 106 (austalide k), 110 (spirohexaline), and 112 (pre-viridicatumtoxin). Thus, these features included diketopiperazines, meroterpenoids, and polyketides, such as indole alkaloids, austalides, and viridicatumtoxin A, a rare tetracycline. |
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معلومات مُعتمدة: | 71/032.390/2022 Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul |
فهرسة مساهمة: | Keywords: OSMAC; bioprospection; metabolomics; molecular networking |
تواريخ الأحداث: | Date Created: 20230225 Latest Revision: 20230301 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9961420 |
DOI: | 10.3390/metabo13020236 |
PMID: | 36837855 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2218-1989 |
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DOI: | 10.3390/metabo13020236 |