دورية أكاديمية
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Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi.

التفاصيل البيبلوغرافية
العنوان: Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi.
المؤلفون: da Silva FMR; Laboratory of Natural Products and Mass Spectrometry (LaPNEM), Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil., Paggi GM; Laboratory of Ecology and Evolutionary Biology (LEBio), Institute of Biosciences, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil., Brust FR; Biofilms and Diversity Laboratory, Faculty of Pharmacy and Biotechnology Center, Federal University of Rio Grande do Sul, Porto Alegre 91501-970, Brazil., Macedo AJ; Biofilms and Diversity Laboratory, Faculty of Pharmacy and Biotechnology Center, Federal University of Rio Grande do Sul, Porto Alegre 91501-970, Brazil., Silva DB; Laboratory of Natural Products and Mass Spectrometry (LaPNEM), Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil.
المصدر: Metabolites [Metabolites] 2023 Feb 05; Vol. 13 (2). Date of Electronic Publication: 2023 Feb 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101578790 Publication Model: Electronic Cited Medium: Print ISSN: 2218-1989 (Print) Linking ISSN: 22181989 NLM ISO Abbreviation: Metabolites Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مستخلص: Metabolomics strategies are important tools to get holistic chemical information from a system, but they are scarcely applied to endophytic fungi to understand their chemical profiles of biosynthesized metabolites. Here Penicillium sp. was cultured using One Strain Many Compounds (OSMAC) conditions as a model system to demonstrate how this strategy can help in understanding metabolic profiles and determining bioactive metabolites with the application of metabolomics and statistical analyses, as well as molecular networking. Penicillium sp. was fermented in different culture media and the crude extracts from mycelial biomass (CEm) and broth (CEb) were obtained, evaluated against bacterial strains ( Staphylococcus aureus and Pseudomonas aeruginosa ), and the metabolomic profiles by LC-DAD-MS were obtained and chemometrics statistical analyses were applied. The CEm and CEb extracts presented different chemical profiles and antibacterial activities; the highest activities observed were against S. aureus from CEm (MIC = 16, 64, and 128 µg/mL). The antibacterial properties from the extracts were impacted for culture media from which the strain was fermented. From the Volcano plot analysis, it was possible to determine statistically the most relevant features for the antibacterial activity, which were also confirmed from biplots of PCA as strong features for the bioactive extracts. These compounds included 75 (13-oxoverruculogen isomer), 78 (austalide P acid), 87 (austalide L or W), 88 (helvamide), 92 (viridicatumtoxin A), 96 (austalide P), 101 (dihydroaustalide K), 106 (austalide k), 110 (spirohexaline), and 112 (pre-viridicatumtoxin). Thus, these features included diketopiperazines, meroterpenoids, and polyketides, such as indole alkaloids, austalides, and viridicatumtoxin A, a rare tetracycline.
References: Metabolites. 2021 Aug 26;11(9):. (PMID: 34564394)
Mar Drugs. 2019 Sep 01;17(9):. (PMID: 31480589)
Mar Drugs. 2020 Jul 06;18(7):. (PMID: 32640519)
Environ Microbiol. 2013 Jul;15(7):2008-18. (PMID: 23419060)
Chembiochem. 2009 Mar 23;10(5):920-8. (PMID: 19226505)
Nucleic Acids Res. 2021 Jul 2;49(W1):W388-W396. (PMID: 34019663)
J Microbiol Biotechnol. 2010 Mar;20(3):622-9. (PMID: 20372037)
Nat Biotechnol. 2016 Aug 9;34(8):828-837. (PMID: 27504778)
ACS Infect Dis. 2020 Jul 10;6(7):1759-1769. (PMID: 32437130)
Chembiochem. 2002 Jul 2;3(7):619-27. (PMID: 12324995)
Appl Microbiol Biotechnol. 2021 Oct;105(20):7731-7741. (PMID: 34568964)
Nat Rev Drug Discov. 2021 Mar;20(3):200-216. (PMID: 33510482)
Planta Med. 2019 May;85(8):619-636. (PMID: 30743274)
Front Microbiol. 2019 Feb 26;10:294. (PMID: 30863377)
Biosci Biotechnol Biochem. 2002 Aug;66(8):1697-705. (PMID: 12353630)
Sci Rep. 2020 Jan 23;10(1):1051. (PMID: 31974423)
Biosci Biotechnol Biochem. 2004 Apr;68(4):820-6. (PMID: 15118309)
Anal Chem. 2009 Apr 15;81(8):3079-86. (PMID: 19301908)
Appl Microbiol Biotechnol. 2020 Jul;104(14):6149-6159. (PMID: 32436033)
Molecules. 2022 May 16;27(10):. (PMID: 35630649)
Front Microbiol. 2021 Sep 01;12:714750. (PMID: 34539608)
Database (Oxford). 2014 Jun 30;2014:. (PMID: 24980130)
Mar Drugs. 2014 Jan 27;12(2):746-56. (PMID: 24473173)
Fitoterapia. 2019 Oct;138:104273. (PMID: 31344395)
J Fungi (Basel). 2017 Jun 28;3(3):. (PMID: 29371552)
J Nat Prod. 2022 Mar 25;85(3):462-478. (PMID: 35112871)
Chem Biol. 2010 May 28;17(5):483-94. (PMID: 20534346)
Braz J Biol. 2020 Oct-Dec;80(4):872-880. (PMID: 31967279)
J Org Chem. 2015 Dec 18;80(24):12501-8. (PMID: 26605854)
Microorganisms. 2021 Jan 19;9(1):. (PMID: 33477910)
Genome Res. 2003 Nov;13(11):2498-504. (PMID: 14597658)
J Fungi (Basel). 2021 Sep 22;7(10):. (PMID: 34682208)
Front Public Health. 2020 Nov 12;8:612844. (PMID: 33282821)
Molecules. 2022 Oct 29;27(21):. (PMID: 36364192)
Biochemistry. 1994 May 17;33(19):5819-28. (PMID: 7514038)
ADMET DMPK. 2022 Mar 04;10(2):131-146. (PMID: 35350115)
Molecules. 2017 Jun 20;22(6):. (PMID: 28632186)
Phytomedicine. 2012 Nov 15;19(14):1270-84. (PMID: 23079233)
J Antibiot (Tokyo). 2008 Oct;61(10):633-7. (PMID: 19168978)
Metabolomics. 2012 Aug;8(4):714-718. (PMID: 22833709)
J Agric Food Chem. 2016 Sep 7;64(35):6659-71. (PMID: 27538469)
Metabolites. 2022 Nov 08;12(11):. (PMID: 36355166)
Bioorg Chem. 2021 Mar;108:104671. (PMID: 33550072)
Food Res Int. 2018 Sep;111:661-673. (PMID: 30007730)
J Nat Prod. 2018 Nov 26;81(11):2501-2511. (PMID: 30407813)
Front Microbiol. 2018 Jul 24;9:1526. (PMID: 30087658)
J Am Chem Soc. 2014 Aug 27;136(34):12137-60. (PMID: 25317739)
Methods Mol Biol. 2017;1550:339-368. (PMID: 28188540)
J Ethnopharmacol. 2011 Sep 1;137(1):327-35. (PMID: 21651970)
PLoS One. 2020 Nov 6;15(11):e0241855. (PMID: 33156835)
Biotechnol Adv. 2020 Mar - Apr;39:107462. (PMID: 31669137)
Mar Drugs. 2021 Jul 21;19(8):. (PMID: 34436242)
Front Microbiol. 2019 Jun 20;10:1404. (PMID: 31281299)
Mar Drugs. 2018 Jul 23;16(7):. (PMID: 30041461)
معلومات مُعتمدة: 71/032.390/2022 Fundação de Apoio ao Desenvolvimento do Ensino, Ciência e Tecnologia do Estado de Mato Grosso do Sul
فهرسة مساهمة: Keywords: OSMAC; bioprospection; metabolomics; molecular networking
تواريخ الأحداث: Date Created: 20230225 Latest Revision: 20230301
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9961420
DOI: 10.3390/metabo13020236
PMID: 36837855
قاعدة البيانات: MEDLINE
الوصف
تدمد:2218-1989
DOI:10.3390/metabo13020236