دورية أكاديمية
Colorimetric Assaying of Exosomal Metabolic Biomarkers.
العنوان: | Colorimetric Assaying of Exosomal Metabolic Biomarkers. |
---|---|
المؤلفون: | Yan E; Center for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637553, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore., Goyal G; Center for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637553, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.; Interdisciplinary Graduate School, Nanyang Technological University, Singapore 637335, Singapore., Yildiz UH; Department of Chemistry, Izmir Institute of Technology, 35430 Izmir, Turkey., Boehm BO; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232, Singapore., Palaniappan A; Center for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637553, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore. |
المصدر: | Molecules (Basel, Switzerland) [Molecules] 2023 Feb 16; Vol. 28 (4). Date of Electronic Publication: 2023 Feb 16. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, c1995- |
مواضيع طبية MeSH: | MicroRNAs*/metabolism , Exosomes*/metabolism, Female ; Humans ; Colorimetry ; Biomarkers/metabolism ; Proteins/metabolism ; Biomarkers, Tumor/metabolism |
مستخلص: | Exosomes released into the extracellular matrix have been reported to contain metabolic biomarkers of various diseases. These intraluminal vesicles are typically found in blood, urine, saliva, breast milk, cerebrospinal fluid, semen, amniotic fluid, and ascites. Analysis of exosomal content with specific profiles of DNA, microRNA, proteins, and lipids can mirror their cellular origin and physiological state. Therefore, exosomal cargos may reflect the physiological processes at cellular level and can potentially be used as biomarkers. Herein, we report an optical detection method for assaying exosomal biomarkers that supersedes the state-of-the-art time consuming and laborious assays such as ELISA and NTA. The proposed assay monitors the changes in optical properties of poly(3-(4-methyl-3'-thienyloxy) propyltriethylammonium bromide) upon interacting with aptamers/peptide nucleic acids in the presence or absence of target biomarkers. As a proof of concept, this study demonstrates facile assaying of microRNA, DNA, and advanced glycation end products in exosomes isolated from human plasma with detection levels of ~1.2, 0.04, and 0.35 fM/exosome, respectively. Thus, the obtained results illustrate that the proposed methodology is applicable for rapid and facile detection of generic exosomal biomarkers for facilitating diseases diagnosis. |
References: | Cell Biosci. 2019 Feb 15;9:19. (PMID: 30815248) Int J Mol Sci. 2021 Jan 04;22(1):. (PMID: 33406804) J Immunol Methods. 2012 Jan 31;375(1-2):207-14. (PMID: 22075275) Front Physiol. 2018 Oct 23;9:1479. (PMID: 30405435) Cancer Metastasis Rev. 2013 Dec;32(3-4):623-42. (PMID: 23709120) Cells. 2019 Apr 03;8(4):. (PMID: 30987213) Nanoscale. 2021 Jan 7;13(1):218-232. (PMID: 33326529) J Steroid Biochem Mol Biol. 2017 May;169:22-28. (PMID: 26877254) ACS Sens. 2020 Aug 28;5(8):2476-2485. (PMID: 32700531) Front Genet. 2019 Mar 14;10:202. (PMID: 30923536) Biol Pharm Bull. 2008 Jun;31(6):1059-62. (PMID: 18520029) J Cancer Res Clin Oncol. 2012 Oct;138(10):1659-66. (PMID: 22638884) Cell Biol Int. 2021 Sep;45(9):1807-1831. (PMID: 33913604) J Control Release. 2018 Oct 10;287:156-166. (PMID: 30165139) Anal Chim Acta. 2019 Aug 20;1066:102-111. (PMID: 31027524) J Prosthet Dent. 2016 Jan;115(1):65-70. (PMID: 26412001) Eur Biophys J. 2017 Dec;46(8):813-820. (PMID: 28866771) Anal Chim Acta. 2020 Jun 1;1114:74-84. (PMID: 32359518) Mol Nutr Food Res. 2015 Sep;59(9):1701-12. (PMID: 26047123) Nat Commun. 2020 Feb 28;11(1):1064. (PMID: 32111836) PLoS One. 2020 Jan 30;15(1):e0227949. (PMID: 31999742) ACS Appl Mater Interfaces. 2020 Jul 15;12(28):31270-31281. (PMID: 32551533) Int Immunol. 2005 Jul;17(7):879-87. (PMID: 15908444) Kidney Int. 2007 Nov;72(9):1095-102. (PMID: 17700640) Expert Rev Proteomics. 2009 Jun;6(3):267-83. (PMID: 19489699) Diagnostics (Basel). 2016 Nov 22;6(4):. (PMID: 27879660) Front Mol Neurosci. 2019 Oct 04;12:240. (PMID: 31636538) J Extracell Vesicles. 2013 Dec 31;2:. (PMID: 24455109) Clin Chim Acta. 2019 Jan;488:165-171. (PMID: 30419221) Proteomics. 2015 Jan;15(2-3):260-71. (PMID: 25307053) J Immunol. 2007 Aug 1;179(3):1969-78. (PMID: 17641064) Adv Clin Chem. 2016;74:103-41. (PMID: 27117662) Nat Cell Biol. 2007 Jun;9(6):654-9. (PMID: 17486113) PLoS One. 2012;7(4):e34653. (PMID: 22506041) Biomacromolecules. 2019 Apr 8;20(4):1709-1718. (PMID: 30856330) J Immunol Methods. 2016 Nov;438:11-20. (PMID: 27568281) J Intern Med. 2015 Sep;278(3):251-63. (PMID: 26153525) Sci Rep. 2014 Aug 29;4:6232. (PMID: 25167841) Am J Pathol. 2021 Dec;191(12):2147-2171. (PMID: 34428422) Semin Cell Dev Biol. 2015 Apr;40:72-81. (PMID: 25724562) J Transl Med. 2011 Jun 08;9:86. (PMID: 21651777) J Mater Chem B. 2020 Jan 28;8(4):592-606. (PMID: 31939473) J Cell Physiol. 1989 Sep;140(3):455-62. (PMID: 2777884) Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13368-73. (PMID: 15326289) J Struct Biol. 2020 Apr 1;210(1):107474. (PMID: 32032755) Mol Cancer. 2018 Sep 20;17(1):137. (PMID: 30236115) |
فهرسة مساهمة: | Keywords: HBV-DNA; exosomal biomarkers; exosome; glycated proteins; mir21; polythiophene |
المشرفين على المادة: | 0 (MicroRNAs) 0 (Biomarkers) 0 (Proteins) 0 (Biomarkers, Tumor) |
تواريخ الأحداث: | Date Created: 20230225 Date Completed: 20230228 Latest Revision: 20230228 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9962048 |
DOI: | 10.3390/molecules28041909 |
PMID: | 36838895 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1420-3049 |
---|---|
DOI: | 10.3390/molecules28041909 |