دورية أكاديمية
Colorimetric Assaying of Exosomal Metabolic Biomarkers.
| العنوان: | Colorimetric Assaying of Exosomal Metabolic Biomarkers. |
|---|---|
| المؤلفون: | Yan E; Center for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637553, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore., Goyal G; Center for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637553, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.; Interdisciplinary Graduate School, Nanyang Technological University, Singapore 637335, Singapore., Yildiz UH; Department of Chemistry, Izmir Institute of Technology, 35430 Izmir, Turkey., Boehm BO; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232, Singapore., Palaniappan A; Center for Biomimetic Sensor Science, Nanyang Technological University, Singapore 637553, Singapore.; School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore. |
| المصدر: | Molecules (Basel, Switzerland) [Molecules] 2023 Feb 16; Vol. 28 (4). Date of Electronic Publication: 2023 Feb 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, c1995- |
| مواضيع طبية MeSH: | MicroRNAs*/metabolism , Exosomes*/metabolism, Female ; Humans ; Colorimetry ; Biomarkers/metabolism ; Proteins/metabolism ; Biomarkers, Tumor/metabolism |
| مستخلص: | Exosomes released into the extracellular matrix have been reported to contain metabolic biomarkers of various diseases. These intraluminal vesicles are typically found in blood, urine, saliva, breast milk, cerebrospinal fluid, semen, amniotic fluid, and ascites. Analysis of exosomal content with specific profiles of DNA, microRNA, proteins, and lipids can mirror their cellular origin and physiological state. Therefore, exosomal cargos may reflect the physiological processes at cellular level and can potentially be used as biomarkers. Herein, we report an optical detection method for assaying exosomal biomarkers that supersedes the state-of-the-art time consuming and laborious assays such as ELISA and NTA. The proposed assay monitors the changes in optical properties of poly(3-(4-methyl-3'-thienyloxy) propyltriethylammonium bromide) upon interacting with aptamers/peptide nucleic acids in the presence or absence of target biomarkers. As a proof of concept, this study demonstrates facile assaying of microRNA, DNA, and advanced glycation end products in exosomes isolated from human plasma with detection levels of ~1.2, 0.04, and 0.35 fM/exosome, respectively. Thus, the obtained results illustrate that the proposed methodology is applicable for rapid and facile detection of generic exosomal biomarkers for facilitating diseases diagnosis. |
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| فهرسة مساهمة: | Keywords: HBV-DNA; exosomal biomarkers; exosome; glycated proteins; mir21; polythiophene |
| المشرفين على المادة: | 0 (MicroRNAs) 0 (Biomarkers) 0 (Proteins) 0 (Biomarkers, Tumor) |
| تواريخ الأحداث: | Date Created: 20230225 Date Completed: 20230228 Latest Revision: 20230228 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9962048 |
| DOI: | 10.3390/molecules28041909 |
| PMID: | 36838895 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1420-3049 |
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| DOI: | 10.3390/molecules28041909 |