دورية أكاديمية
In vitro differentiation of myeloid suppressor cells (MDSC-like) from an immature myelomonocytic precursor THP-1.
| العنوان: | In vitro differentiation of myeloid suppressor cells (MDSC-like) from an immature myelomonocytic precursor THP-1. |
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| المؤلفون: | Varela VA; Faculdade Israelita de Ciências da Saúde Albert Einstein (FICSAE), Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., da Silva Heinen LB; Hospital Sírio Libanês, São Paulo, SP, Brazil., Marti LC; Faculdade Israelita de Ciências da Saúde Albert Einstein (FICSAE), Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Caraciolo VB; Faculdade Israelita de Ciências da Saúde Albert Einstein (FICSAE), Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Datoguia TS; Faculdade Israelita de Ciências da Saúde Albert Einstein (FICSAE), Hospital Israelita Albert Einstein, São Paulo, SP, Brazil., Amano MT; Hospital Sírio Libanês, São Paulo, SP, Brazil; Department of Clinical and Experimental Oncology, Federal University of São Paulo (UNIFESP), São Paulo, SP, Brazil., Pereira WO; Faculdade Israelita de Ciências da Saúde Albert Einstein (FICSAE), Hospital Israelita Albert Einstein, São Paulo, SP, Brazil. Electronic address: welbert.pereira@einstein.br. |
| المصدر: | Journal of immunological methods [J Immunol Methods] 2023 Apr; Vol. 515, pp. 113441. Date of Electronic Publication: 2023 Feb 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 1305440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7905 (Electronic) Linking ISSN: 00221759 NLM ISO Abbreviation: J Immunol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam, North-Holand, |
| مواضيع طبية MeSH: | Myeloid-Derived Suppressor Cells*/metabolism, Interleukin-4/metabolism ; Myeloid Cells/metabolism ; Cytokines/metabolism ; Cell Differentiation ; Granulocyte Colony-Stimulating Factor/metabolism |
| مستخلص: | Background: Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population with a potent suppressor profile that regulates immune responses. These cells are one of the main components of the microenvironment of several diseases, including solid and hematologic tumors, autoimmunities, and chronic inflammation. However, their wide use in studies is limited due to they comprehend a rare population, which is difficult to isolate, expand, differentiate, and maintain in culture. Additionally, this population has a complex phenotypic and functional characterization. Objective: To develop a protocol for the in vitro production of MDSC-like population from the differentiation of the immature myeloid cell line THP-1. Methods: We stimulated THP-1 with G-CSF (100 ng/mL) and IL-4 (20 ng/mL) for seven days to differentiate into the MDSC-like profile. At the end of the protocol, we characterized these cells phenotypically and functionally by immunophenotyping, gene expression analysis, cytokine release dosage, lymphocyte proliferation, and NK-mediated killing essays. Results: We differentiate THP-1 cells in an MDSC-like population, named THP1-MDSC-like, which presented immunophenotyping and gene expression profiles compatible with that described in the literature. Furthermore, we verified that this phenotypic and functional differentiation did not deviate to a macrophage profile of M1 or M2. These THP1-MDSC-like cells secreted several immunoregulatory cytokines into the microenvironment, consistent with the suppressor profile related to MDSC. In addition, the supernatant of these cells decreased the proliferation of activated lymphocytes and impaired the apoptosis of leukemic cells induced by NK cells. Conclusions: We developed an effective protocol for MDSC in vitro production from the differentiation of the immature myeloid cell line THP-1 induced by G-CSF and IL-4. Furthermore, we demonstrated that THP1-MDSC-like suppressor cells contribute to the immune escape of AML cells. Potentially, these THP1-MDSC-like cells can be applied on a large-scale platform, thus being able to impact the course of several studies and models such as cancer, immunodeficiencies, autoimmunity, and chronic inflammation. Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Acute myeloid leukemia; Cell differentiation; MDSC; NK cell; Suppressor cells; THP-1 |
| المشرفين على المادة: | 207137-56-2 (Interleukin-4) 0 (Cytokines) 143011-72-7 (Granulocyte Colony-Stimulating Factor) |
| تواريخ الأحداث: | Date Created: 20230227 Date Completed: 20230403 Latest Revision: 20230622 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jim.2023.113441 |
| PMID: | 36848984 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7905 |
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| DOI: | 10.1016/j.jim.2023.113441 |