دورية أكاديمية
أعرض في EDS

A Combined Approach for Detection of Ovine Small Ruminant Retrovirus Co-Infections.

التفاصيل البيبلوغرافية
العنوان: A Combined Approach for Detection of Ovine Small Ruminant Retrovirus Co-Infections.
المؤلفون: Rosato G; Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, CH 8057 Zurich, Switzerland., Abril C; Institute of Virology and Immunology IVI, in Cooperation with the Vetsuisse-Faculty of the University of Bern, 3012 Bern, Switzerland., Hilbe M; Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, CH 8057 Zurich, Switzerland., Seehusen F; Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, CH 8057 Zurich, Switzerland.
المصدر: Viruses [Viruses] 2023 Jan 28; Vol. 15 (2). Date of Electronic Publication: 2023 Jan 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Coinfection*/veterinary , Retroviridae Infections* , Arthritis-Encephalitis Virus, Caprine* , Jaagsiekte sheep retrovirus*, Sheep ; Animals ; Retroviridae ; Ruminants ; Antibodies ; Real-Time Polymerase Chain Reaction
مستخلص: Jaagsiekte retrovirus (JSRV)-induced ovine pulmonary adenocarcinoma (OPA) is an important ovine respiratory disease in Switzerland. Furthermore, ovine lungs with OPA frequently exhibited lesions suggestive of maedi-visna virus (MVV) or caprine arthritis encephalitis virus (CAEV) infection, indicating that co-morbidities might occur. Lungs and pulmonary lymph nodes were sampled from suspected OPA cases, inflammatory lung lesions and control lungs (total of 110 cases). Tissues were (a) processed for histology and immunohistochemistry (IHC), and (b) underwent DNA extraction and real-time PCR for JSRV, MVV and CAEV. Peptide sequences were used to generate virus-specific customized polyclonal antibodies. PCR-positive OPA cases and formalin-fixed and paraffin-embedded MVV- and CAEV-infected synovial cell pellets served as positive controls. Fifty-two lungs were histologically diagnosed with OPA. Histological evidence of MVV/CAEV infection was detected in 25 lungs. JSRV was detected by PCR in 84% of the suspected OPA cases; six were co-infected with MVV and one with CAEV. MVV was detected by PCR in 14 cases, and four lungs were positive for CAEV. Three lungs had MVV/CAEV co-infection. In IHC, JSRV was detected in 91% of the PCR-positive cases, whereas MVV and CAEV immunoreactivity was seen in all PCR-positive lungs. Although PCR showed a higher sensitivity compared to IHC, the combined approach allows for investigations on viral cell tropism and pathogenic processes in co-morbidities, including their potential interdependency. Furthermore, an immunohistochemical tool for specific differentiation of MVV and/or CAEV infection was implemented.
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فهرسة مساهمة: Keywords: CAEV; JSRV; MVV; co-infections; diagnostic; ovine pulmonary adenocarcinoma (OPA); viral cell tropism
المشرفين على المادة: 0 (Antibodies)
تواريخ الأحداث: Date Created: 20230228 Date Completed: 20230301 Latest Revision: 20230411
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9958757
DOI: 10.3390/v15020376
PMID: 36851589
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v15020376