دورية أكاديمية
Protocol to transfer epithelial acini between different extracellular matrices and orient fibril organization.
| العنوان: | Protocol to transfer epithelial acini between different extracellular matrices and orient fibril organization. |
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| المؤلفون: | Katsuno-Kambe H; Division of Cell and Developmental Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: hiro.katsukatsu@gmail.com., Hudson JE; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Voges HK; QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia., Yap AS; Division of Cell and Developmental Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia. Electronic address: a.yap@uq.edu.au. |
| المصدر: | STAR protocols [STAR Protoc] 2023 Mar 17; Vol. 4 (1), pp. 102077. Date of Electronic Publication: 2023 Jan 29. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Extracellular Matrix*/metabolism , Collagen*/chemistry, Elasticity |
| مستخلص: | Extracellular matrix (ECM) provides fundamental support for epithelial tissues and controls cell function. The chemistry and mechanical properties of ECM components, including stiffness, elasticity, and fibrillar organization, influence epithelial tissue responses. Here we present a protocol describing the culture and transfer of epithelial acini from Matrigel to collagen gel and an approach to axially align the collagen fibrils by the external gel stretching. This protocol uses the acini of MCF10A cells and needs to be modified for different cell lines. For complete details on the use and execution of this protocol, please refer to Katsuno-Kambe et al. (2021). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cancer; Cell Biology; Cell Culture |
| المشرفين على المادة: | 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20230228 Date Completed: 20231023 Latest Revision: 20231024 |
| رمز التحديث: | 20231025 |
| مُعرف محوري في PubMed: | PMC9898047 |
| DOI: | 10.1016/j.xpro.2023.102077 |
| PMID: | 36853715 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2023.102077 |