دورية أكاديمية
أعرض في EDS

Composition, structure, and functional shifts of prokaryotic communities in response to co-composting of various nitrogenous green feedstocks.

التفاصيل البيبلوغرافية
العنوان: Composition, structure, and functional shifts of prokaryotic communities in response to co-composting of various nitrogenous green feedstocks.
المؤلفون: Matheri F; International Centre for Insect Physiology and Ecology (icipe), P.O. Box 30772 - 00100, Nairobi, Kenya.; Kenyatta University (KU), P.O. Box 43844 - 00100, Nairobi, Kenya., Kambura AK; Taita Taveta University (TTU), P.O. Box 635 - 80300, Voi, Kenya., Mwangi M; Kenyatta University (KU), P.O. Box 43844 - 00100, Nairobi, Kenya., Ongeso N; The University of Nairobi (UON), P.O. Box 30197, GPO, Nairobi, Kenya., Karanja E; International Centre for Insect Physiology and Ecology (icipe), P.O. Box 30772 - 00100, Nairobi, Kenya., Adamtey N; International Division, Research Institute of Organic Agriculture (FiBL), Ackerstrasse 775, CH-5070, Frick, Switzerland., Mwangi EK; Kenyatta University (KU), P.O. Box 43844 - 00100, Nairobi, Kenya., Mwangi E; International Centre for Insect Physiology and Ecology (icipe), P.O. Box 30772 - 00100, Nairobi, Kenya., Tanga C; International Centre for Insect Physiology and Ecology (icipe), P.O. Box 30772 - 00100, Nairobi, Kenya., Musyoka MW; International Centre for Insect Physiology and Ecology (icipe), P.O. Box 30772 - 00100, Nairobi, Kenya.; Biofarms (K) Limited, P.O. Box 7277 - 01000, Thika, Kenya., Runo S; Kenyatta University (KU), P.O. Box 43844 - 00100, Nairobi, Kenya. smruno@gmail.com.
المصدر: BMC microbiology [BMC Microbiol] 2023 Mar 02; Vol. 23 (1), pp. 50. Date of Electronic Publication: 2023 Mar 02.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Composting*, RNA, Ribosomal, 16S ; Phylogeny ; Prokaryotic Cells ; Carbohydrates
مستخلص: Background: Thermophilic composting is a promising method of sanitizing pathogens in manure and a source of agriculturally important thermostable enzymes and microorganisms from organic wastes. Despite the extensive studies on compost prokaryotes, shifts in microbial profiles under the influence of various green materials and composting days are still not well understood, considering the complexity of the green material sources. Here, the effect of regimens of green composting material on the diversity, abundance, and metabolic capacity of prokaryotic communities in a thermophilic compost environment was examined.
Methods: Total community 16S rRNA was recovered from triplicate compost samples of Lantana-based, Tithonia-based, Grass-based, and mixed (Lantana + Tithonia + Grass)- based at 21, 42, 63, and 84 days of composting. The 16S rRNA was sequenced using the Illumina Miseq platform. Bioinformatics analysis was done using Divisive Amplicon Denoising Algorithm version 2 (DADA2) R version 4.1 and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States version 2 (PICRUSt2) pipelines for community structure and metabolic profiles, respectively. In DADA2, prokaryotic classification was done using the Refseq-ribosomal database project (RDP) and SILVA version 138 databases.
Results: Our results showed apparent differences in prokaryotic community structure for total diversity and abundance within the four compost regimens and composting days. The study showed that the most prevalent phyla during composting included Acidobacteriota, Actinobacteriota, Bacteroidota, Chloroflexi, and Proteobacteria. Additionally, there were differences in the overall diversity of metabolic pathways but no significant differences among the various compost treatments on major metabolic pathways like carbohydrate biosynthesis, carbohydrate degradation, and nitrogen biosynthesis.
Conclusion: Various sources of green material affect the succession of compost nutrients and prokaryotic communities. The similarity of amounts of nutrients, such as total Nitrogen, at the end of the composting process, despite differences in feedstock material, indicates a significant influence of composting days on the stability of nutrients during composting.
(© 2023. The Author(s).)
References: Front Bioeng Biotechnol. 2019 Sep 03;7:209. (PMID: 31552235)
Sci Rep. 2016 Dec 12;6:38915. (PMID: 27941956)
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6. (PMID: 23193283)
J Appl Microbiol. 2005;99(4):816-28. (PMID: 16162232)
PLoS One. 2015 Oct 05;10(10):e0138909. (PMID: 26436656)
FEMS Microbiol Ecol. 2008 Jul;65(1):133-44. (PMID: 18537836)
J Exp Bot. 2009;60(10):2923-31. (PMID: 19325167)
PLoS One. 2017 Oct 23;12(10):e0186051. (PMID: 29059245)
Science. 2014 Aug 29;345(6200):1052-4. (PMID: 25170152)
Indian J Pharm Sci. 2009 Sep;71(5):589-93. (PMID: 20502587)
Metab Eng. 2017 Jan;39:200-208. (PMID: 27939572)
Sci Rep. 2020 Feb 4;10(1):1759. (PMID: 32019965)
PLoS One. 2013 Nov 19;8(11):e79786. (PMID: 24260300)
Genes (Basel). 2021 Apr 13;12(4):. (PMID: 33924545)
Pharmaceutics. 2021 Aug 16;13(8):. (PMID: 34452226)
Infect Ecol Epidemiol. 2016 Nov 08;6:32453. (PMID: 27834174)
PLoS One. 2020 Jan 16;15(1):e0227434. (PMID: 31945086)
Metabolites. 2021 Jan 12;11(1):. (PMID: 33445571)
PLoS One. 2010 Jan 21;5(1):e8812. (PMID: 20098679)
Microbiome. 2017 Aug 16;5(1):103. (PMID: 28814344)
Bioresour Technol. 2020 Feb;298:122550. (PMID: 31837577)
Front Microbiol. 2022 Feb 18;13:824834. (PMID: 35250940)
Nat Methods. 2010 Sep;7(9):668-9. (PMID: 20805793)
Bioinformatics. 2014 Nov 1;30(21):3123-4. (PMID: 25061070)
BMC Microbiol. 2013 May 07;13:99. (PMID: 23651653)
ISME J. 2017 Dec;11(12):2639-2643. (PMID: 28731476)
FEMS Microbiol Rev. 2000 Oct;24(4):403-27. (PMID: 10978544)
J Air Waste Manag Assoc. 2017 Mar;67(3):313-321. (PMID: 27650130)
Nat Biotechnol. 2020 Jun;38(6):685-688. (PMID: 32483366)
Front Microbiol. 2019 Mar 18;10:529. (PMID: 30936861)
Curr Opin Biotechnol. 2021 Feb;67:184-191. (PMID: 33592536)
PLoS One. 2016 Jun 14;11(6):e0157539. (PMID: 27300323)
ISME J. 2017 Jun;11(6):1434-1446. (PMID: 28186498)
Sci Total Environ. 2021 Jan 10;751:141607. (PMID: 32871314)
Bioresour Technol. 2011 Dec;102(23):10965-72. (PMID: 21996482)
Saudi J Biol Sci. 2020 Dec;27(12):3634-3640. (PMID: 33304174)
Appl Environ Microbiol. 2010 Nov;76(22):7575-87. (PMID: 20870784)
PLoS One. 2020 Aug 13;15(8):e0236574. (PMID: 32790770)
Front Microbiol. 2018 Apr 06;9:683. (PMID: 29681896)
Bioresour Technol. 2021 Nov;340:125652. (PMID: 34332446)
Microb Genom. 2020 Aug;6(8):. (PMID: 32706331)
Front Microbiol. 2011 Aug 01;2:165. (PMID: 21866228)
3 Biotech. 2018 Jul;8(7):301. (PMID: 29963361)
معلومات مُعتمدة: 91712524 Deutscher Akademischer Austauschdienst; B2222F-3001-100 Direktion für Entwicklung und Zusammenarbeit; 91712524 Kenyatta University
فهرسة مساهمة: Keywords: Composting; Grass; Lantana; Microbe-microbe interactions; Organic farming; Tithonia
المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
0 (Carbohydrates)
SCR Disease Name: Severe combined immunodeficiency due to adenosine deaminase deficiency
تواريخ الأحداث: Date Created: 20230301 Date Completed: 20230303 Latest Revision: 20230313
رمز التحديث: 20230314
مُعرف محوري في PubMed: PMC9979578
DOI: 10.1186/s12866-023-02798-w
PMID: 36859170
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2180
DOI:10.1186/s12866-023-02798-w