دورية أكاديمية
Genome transfer technique for bovine embryo production using the metaphase plate and polar body.
العنوان: | Genome transfer technique for bovine embryo production using the metaphase plate and polar body. |
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المؤلفون: | Dode MAN; University of Brasilia, DF, Brasília, Brazil. margot.dode@embrapa.br.; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil. margot.dode@embrapa.br., Caixeta FMC; University of Brasilia, DF, Brasília, Brazil., Vargas LN; Federal University of Uberlândia, Uberlândia, MG, Brazil., Leme LO; Federal University of Espírito Santo, Alegre, ES, Brazil., Kawamoto TS; Federal University of Uberlândia, Uberlândia, MG, Brazil., Fidelis AAG; University of Brasilia, DF, Brasília, Brazil., Franco MM; Federal University of Uberlândia, Uberlândia, MG, Brazil.; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil. |
المصدر: | Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2023 Apr; Vol. 40 (4), pp. 943-951. Date of Electronic Publication: 2023 Mar 03. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 9206495 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-7330 (Electronic) Linking ISSN: 10580468 NLM ISO Abbreviation: J Assist Reprod Genet Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2004- >: Amsterdam : Springer Original Publication: New York : Plenum Press, c1992- |
مواضيع طبية MeSH: | Polar Bodies* , Fertilization in Vitro*/methods, Humans ; Male ; Animals ; Cattle ; Mice ; Metaphase/genetics ; Cryopreservation/methods ; Semen ; Oocytes ; Blastocyst |
مستخلص: | Despite many studies in humans and mice using genome transfer (GT), there are few reports using this technique in oocytes of wild or domestic animals. Therefore, we aimed to establish a GT technique in bovine oocytes using the metaphase plate (MP) and polar body (PB) as the sources of genetic material. In the first experiment, GT was established using MP (GT-MP), and a sperm concentration of 1 × 10 6 or 0.5 × 10 6 spermatozoa/ml gave similar fertilization rates. The cleavage rate (50%) and blastocyst rate (13.6%) in the GT-MP group was lower than that of the in vitro production control group (80.2% and 32.6%, respectively). The second experiment evaluated the same parameters using PB instead of MP; the GT-PB group had lower fertilization (82.3% vs. 96.2%) and blastocyst (7.7% vs. 36.8%) rates than the control group. No differences in the amount of mitochondrial DNA (mtDNA) were observed between groups. Finally, GT-MP was performed using vitrified oocytes (GT-MPV) as a source of genetic material. The cleavage rate of the GT-MPV group (68.4%) was similar to that of the vitrified oocytes (VIT) control group (70.0%) and to that of the control IVP group (81.25%, P < 0.05). The blastocyst rate of GT-MPV (15.7) did not differ neither from the VIT control group (5.0%) nor from the IVP control group (35.7%). The results suggested that the structures reconstructed by the GT-MPV and GT-PB technique develop in embryos even if vitrified oocytes are used. (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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فهرسة مساهمة: | Keywords: Cryotop; In vitro embryo production; Micromanipulation; Oocyte |
تواريخ الأحداث: | Date Created: 20230302 Date Completed: 20230529 Latest Revision: 20240402 |
رمز التحديث: | 20240402 |
مُعرف محوري في PubMed: | PMC10224876 |
DOI: | 10.1007/s10815-023-02758-3 |
PMID: | 36864182 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1573-7330 |
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DOI: | 10.1007/s10815-023-02758-3 |