دورية أكاديمية
أعرض في EDS

Genome transfer technique for bovine embryo production using the metaphase plate and polar body.

التفاصيل البيبلوغرافية
العنوان: Genome transfer technique for bovine embryo production using the metaphase plate and polar body.
المؤلفون: Dode MAN; University of Brasilia, DF, Brasília, Brazil. margot.dode@embrapa.br.; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil. margot.dode@embrapa.br., Caixeta FMC; University of Brasilia, DF, Brasília, Brazil., Vargas LN; Federal University of Uberlândia, Uberlândia, MG, Brazil., Leme LO; Federal University of Espírito Santo, Alegre, ES, Brazil., Kawamoto TS; Federal University of Uberlândia, Uberlândia, MG, Brazil., Fidelis AAG; University of Brasilia, DF, Brasília, Brazil., Franco MM; Federal University of Uberlândia, Uberlândia, MG, Brazil.; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.
المصدر: Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2023 Apr; Vol. 40 (4), pp. 943-951. Date of Electronic Publication: 2023 Mar 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Netherlands NLM ID: 9206495 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-7330 (Electronic) Linking ISSN: 10580468 NLM ISO Abbreviation: J Assist Reprod Genet Subsets: MEDLINE
أسماء مطبوعة: Publication: <2004- >: Amsterdam : Springer
Original Publication: New York : Plenum Press, c1992-
مواضيع طبية MeSH: Polar Bodies* , Fertilization in Vitro*/methods, Humans ; Male ; Animals ; Cattle ; Mice ; Metaphase/genetics ; Cryopreservation/methods ; Semen ; Oocytes ; Blastocyst
مستخلص: Despite many studies in humans and mice using genome transfer (GT), there are few reports using this technique in oocytes of wild or domestic animals. Therefore, we aimed to establish a GT technique in bovine oocytes using the metaphase plate (MP) and polar body (PB) as the sources of genetic material. In the first experiment, GT was established using MP (GT-MP), and a sperm concentration of 1 × 10 6 or 0.5 × 10 6 spermatozoa/ml gave similar fertilization rates. The cleavage rate (50%) and blastocyst rate (13.6%) in the GT-MP group was lower than that of the in vitro production control group (80.2% and 32.6%, respectively). The second experiment evaluated the same parameters using PB instead of MP; the GT-PB group had lower fertilization (82.3% vs. 96.2%) and blastocyst (7.7% vs. 36.8%) rates than the control group. No differences in the amount of mitochondrial DNA (mtDNA) were observed between groups. Finally, GT-MP was performed using vitrified oocytes (GT-MPV) as a source of genetic material. The cleavage rate of the GT-MPV group (68.4%) was similar to that of the vitrified oocytes (VIT) control group (70.0%) and to that of the control IVP group (81.25%, P < 0.05). The blastocyst rate of GT-MPV (15.7) did not differ neither from the VIT control group (5.0%) nor from the IVP control group (35.7%). The results suggested that the structures reconstructed by the GT-MPV and GT-PB technique develop in embryos even if vitrified oocytes are used.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
References: Reprod Biomed Online. 2005 Sep;11(3):300-8. (PMID: 16176668)
PLoS One. 2015 May 12;10(5):e0126801. (PMID: 25965267)
Biopreserv Biobank. 2019;17(1):76-83. (PMID: 30256133)
Cryobiology. 2014 Oct;69(2):256-65. (PMID: 25106744)
Reproduction. 2018 Jul;156(1):F11-F27. (PMID: 29581237)
J Assist Reprod Genet. 2018 Jul;35(7):1161-1168. (PMID: 29802518)
PLoS One. 2015 Jun 24;10(6):e0130164. (PMID: 26107169)
Sci Rep. 2016 Jan 27;6:19827. (PMID: 26813698)
Reprod Biomed Online. 2017 Apr;34(4):361-368. (PMID: 28385334)
Theriogenology. 2020 Jul 15;151:137-143. (PMID: 32361180)
Reprod Domest Anim. 2009 Jun;44(3):480-8. (PMID: 18992089)
Cell. 2014 Jun 19;157(7):1591-604. (PMID: 24949971)
Reprod Fertil Dev. 2015 Apr 22;:. (PMID: 25897945)
Theriogenology. 1995 Oct 15;44(6):859-69. (PMID: 16727781)
Theriogenology. 2017 Feb;89:47-57. (PMID: 28043370)
Nature. 2013 Jan 31;493(7434):632-7. (PMID: 23254936)
Reprod Biomed Online. 2014 Dec;29(6):708-16. (PMID: 25444504)
J Vet Med Sci. 2019 Jan 8;81(1):84-90. (PMID: 30473579)
Reprod Med Biol. 2020 Nov 03;20(1):53-61. (PMID: 33488283)
Annu Rev Genet. 2016 Nov 23;50:93-111. (PMID: 27617973)
J Assist Reprod Genet. 2017 May;34(5):563-571. (PMID: 28190214)
Reprod Fertil Dev. 2011;23(3):424-32. (PMID: 21426860)
Reprod Biomed Online. 2014 Mar;28(3):401-4. (PMID: 24316045)
Mitochondrion. 2014 Sep;18:27-33. (PMID: 25229667)
PLoS One. 2018 Jun 18;13(6):e0198742. (PMID: 29912910)
Reprod Biomed Online. 2015 Jul;31(1):71-8. (PMID: 25985993)
Theriogenology. 2009 May;71(8):1289-97. (PMID: 19230963)
Cell Res. 2021 Feb;31(2):233-236. (PMID: 32724085)
Fertil Steril. 2010 Jan;93(1):229-38. (PMID: 18976992)
Nat Protoc. 2010 Jun;5(6):1138-47. (PMID: 20539289)
Biol Reprod. 2013 Jun 06;88(6):139. (PMID: 23575153)
Theriogenology. 2022 Mar 1;180:63-71. (PMID: 34953350)
Nature. 2009 Sep 17;461(7262):367-72. (PMID: 19710649)
Fertil Steril. 2009 Oct;92(4):1306-1311. (PMID: 18930218)
Cryobiology. 2012 Dec;65(3):319-25. (PMID: 22981976)
Fertil Steril. 2015 Dec;104(6):1426-34.e1-8. (PMID: 26353081)
Cryo Letters. 2019 Mar/Apr;40(2):123-128. (PMID: 31017612)
J Reprod Dev. 2015;61(5):431-7. (PMID: 26119929)
Nature. 2013 Jan 31;493(7434):627-31. (PMID: 23103867)
Int J Mol Sci. 2020 Oct 13;21(20):. (PMID: 33066129)
Cryo Letters. 2016 Jan-Feb;37(1):27-33. (PMID: 26964022)
Cryobiology. 2014 Dec;69(3):496-9. (PMID: 25224047)
Theriogenology. 2016 Nov;86(8):2054-62. (PMID: 27523724)
Theriogenology. 1999 Sep;52(4):683-700. (PMID: 10734366)
فهرسة مساهمة: Keywords: Cryotop; In vitro embryo production; Micromanipulation; Oocyte
تواريخ الأحداث: Date Created: 20230302 Date Completed: 20230529 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC10224876
DOI: 10.1007/s10815-023-02758-3
PMID: 36864182
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-7330
DOI:10.1007/s10815-023-02758-3